The use of biological control agents (BCA), alone or in combination with other management measures, has gained attention over the past decades, driven by need to seek for sustainable and eco-friendly alternatives confront plant pathogens. rhizosphere olive (Olea europaea L.) plants is a source bacteria potential as biocontrol tools against Verticillium wilt (VWO) caused dahliae Kleb. A collection bacterial isolates from healthy nursery-produced (cultivar Picual, susceptible VWO) was...

Pseudomonas fluorescens strain PICF7 is a native endophyte of olive roots. Previous studies have shown this motile, Gram-negative, non-sporulating bacterium an effective biocontrol agent against the soil-borne fungus Verticillium dahliae, causal one most devastating diseases for (Olea europaea L.) cultivation. Here, we announce and describe complete genome sequence consisting circular chromosome 6,136,735 bp that encodes 5,567 protein-coding genes 88 RNA-only encoding genes. Genome analysis...

A novel metabolite, 3-hydroxy-5-(6-isopropyl-3-methylene-3,4,4a,5,6,7,8,8a-octahydronaphthalen-2-yl)dihydrofuran-2-one, trivially named cerinolactone (1), has been isolated from culture filtrates of Trichoderma cerinum together with three known butenolides containing the 3,4-dialkylfuran-2(5H)-one nucleus, harzianolide (2), T39butenolide (3), and dehydroharzianolide (4). The structure 1 was determined by spectroscopic methods, including UV, MS, 1D 2D NMR analyses. In vitro tests purified...

The use of biological control agents (BCAs) is interest within an integrated management strategy Verticillium wilt olive (VWO) caused by the soil-borne fungus dahliae Kleb. Previous studies have shown that root/rhizosphere healthy plants important reservoir microorganisms displaying biocontrol activity against VWO (i.e., Pseudomonas strains PICF7 and PIC141). Moreover, these BCAs are already adapted to ecological niche where they deployed. Three novel bacteria (strains PIC28, PIC73 PIC167)...

Fifty-six bulk isolates of Trichoderma spp. from avocado (Persea americana Mill.), carnation (Dianthus caryophyllus L.), litchi (Litchi chinensis Sonn), rice (Oryza sativa L.) and sugar beet (Beta vulgaris crops located in southern Spain were evaluated for antagonism against one isolate Rosellinia necatrix Prill. Isolates both types fungi tested dual cellophane culture. The origin, cultural characteristics, overgrowth sporulation staining growth medium recorded. As a result, 21 selected...

Interest in pistachios has increased recent years due to their healthy nutritional profile and high profitability. In pistachio trees, as other woody crops, the volume of canopy is a key factor that affects crop load, water requirements, quality. However, canopy/crown monitoring time-consuming labor-intensive, it traditionally carried out by measuring tree dimensions field. Therefore, methods for rapid characterization are needed providing accurate information can be used management...

Consumers are increasingly demanding higher quality and safety standards for the products they consume, one of this is wheat flour, basis a wide variety processed products. This major component in diet many communities can be contaminated by microorganisms before grain harvest, or during storage right processing. These include several fungal species, which produce mycotoxins, secondary metabolites that cause severe acute chronic disorders. Yet, we still know little about overall composition...

A homothallic Phytophthora species was found to be consistently associated with a rot of mature fruits two local cultivars olive ( Olea europaea ) in Calabria, southern Italy. The phylogenetic analysis sequences the ITS 1‐5.8S‐ 2 region and cox1 gene enabled its identification as new clade 2, basal position compared previously described subclades. is formally epithet oleae , referring natural matrix from which it isolated. unique combination molecular morphological characters clearly...

Grapevine trunk diseases (GTDs) are a major threat to the wine and grape industry. The aim of study was investigate antifungal activity against Neofusicoccum parvum, Diplodia seriata, Botryosphaeria dothidea ε-polylysine, chitosan oligomers, their conjugates, Streptomyces rochei S. lavendofoliae culture filtrates, binary mixtures with oligomers. In vitro mycelial growth inhibition tests suggest that efficacy these treatments, in particular those based on ε-polylysine ε-polylysine:chitosan...

Grapevine is highly susceptible to fungal diseases, whose incidence and severity increase due climate change. The present work focuses on the assessment of eight combinations natural products with chitosan oligomers fungicidal capacity that may be effective in integrated control powdery mildew, compliance Article 14 European Directive 2009/128/EC. Their efficacy was evaluated field conditions against infections, a plot high disease pressure during growing season (assaying both foliar or root...

A total of 248 UAV RGB images were taken in the summer 2021 over a representative pistachio orchard Spain (X: 341450.3, Y: 4589731.8; ETRS89/UTM zone 30N). It is 2.03 ha plot, planted 2016 with Pistacia vera L. cv. Kerman grafted on UCB rootstock, NE–SW orientation and 7 × 6 m triangular planting pattern. The ground was kept free any weeds that could affect image processing. photos (provided JPG format) using DJI Phantom Advance quadcopter two flight missions: one planned to take nadir (β =...

An increased incidence of dieback from branches in several avocado orchards southern Spain was observed 2014. Surveys were conducted May to October 2014, sampling the affected isolate causal agents. A total 68 fungal isolates, recovered ten orchards, identified, by morphological characterisation and DNA sequencing, as belonging genera: Neofusicoccum parvum (50%), Colletotrichum gloeosporioides (17.6%), luteum (16.2%), australe (13.2%), mediterraneum (1.5%) Lasiodiplodia theobromae (1.5%)....

Host resistance is the most practical, long-term, and economically efficient disease control measure for Verticillium wilt in olive caused by xylem-invading fungus dahliae ( Vd ), it at core of integrated management. Plant’s microbiome site infection may have an influence on host reaction to pathogens; however, role xylem microbial communities has been overlooked remains unexplored date. This research was focused elucidating whether vitro propagation alter diversity composition...

White root rot, caused by Rosellinia necatrix , is one of the most important diseases in avocado orchards and particularly widespread on Mediterranean seaboard southern Spain. In this study, presence pathogen soil samples collected from base 47 plants showing different symptoms canopy decline was assessed with a molecular detection method based real‐time Scorpion PCR. Results were compared traditional isolation R. roots and/or bark. The fungus isolated 24 37 (cycle threshold values 25·8 to...

The olive fruit fly (OFF), Bactrocera oleae is the most devastating pest affecting worldwide. Previous investigations have addressed fungal microbiome associated with drupes or B. oleae, but impact of insect on communities remains undescribed. In present work, drupes, infested and non-infested by OFF, was investigated in four different localities cultivars. Olive infestations caused a general reduction diversity, higher quantity total DNA an increase taxa that remained unidentified had...