A5087790148- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- Photosynthetic Processes and Mechanisms
- Cassava research and cyanide
- Plant responses to elevated CO2
- Sunflower and Safflower Cultivation
- Nitric Oxide and Endothelin Effects
- Essential Oils and Antimicrobial Activity
- Aluminum toxicity and tolerance in plants and animals
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Edible Oils Quality and Analysis
- Polyamine Metabolism and Applications
- GABA and Rice Research
- Synthesis and bioactivity of alkaloids
- Redox biology and oxidative stress
- Magnetic and Electromagnetic Effects
- Nematode management and characterization studies
- Genetic diversity and population structure
- Biological Activity of Diterpenoids and Biflavonoids
- Selenium in Biological Systems
- Aldose Reductase and Taurine
Universidad de Jaén
2004-2020
ADA University
1981
Universidad de Granada
1981
Nitrosative stress has become a usual term in the physiology of nitric oxide mammalian systems. However, plants there is much less information on this type stress. Using olive leaves as experimental model, effect salinity potential induction nitrosative was studied. The enzymatic l ‐arginine‐dependent production (NOS activity) measured by ozone chemiluminiscence. specific activity NOS 0.280 nmol NO mg −1 protein min , and dependent ‐arginine, NADPH calcium. Salt (200 mM NaCl) caused an...
ABSTRACT NADPH is an important molecule in the redox balance of cell. In this paper, using olive tissue cultures as a model function NADPH‐generating dehydrogenases mechanism oxidative stress induced by severe salinity conditions was studied. When ( Olea europaea ) plants were grown with 200 m NaCl, 40% reduction leaf fresh weight produced. The content non‐enzymatic antioxidants such ascorbate and glutathione diminished between 20% to 39%, whereas H 2 O increased threefold. contrast,...
Tyrosine nitration is recognized as an important post-translational protein modification in animal cells that can be used indicator of a nitrosative process. However, plant systems, there scant information on proteins undergo this In sunflower hypocotyls, the content tyrosine (NO(2)-Tyr) and identification nitrated were studied by high-performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) proteomic approaches, respectively. addition, cell localization nitrotyrosine...
Nitric oxide (·NO) has been shown to participate in plant response against pathogen infection; however, less is known of the participation other NO-derived molecules designated as reactive nitrogen species (RNS). Using two sunflower (Helianthus annuus L.) cultivars with different sensitivity infection by Plasmopara halstedii, we studied key components involved RNS and ROS metabolism. We analyzed superoxide radical production, hydrogen peroxide content, l-arginine-dependent nitric synthase...
Nitric oxide (NO) and related molecules such as peroxynitrite, S-nitrosoglutathione (GSNO), nitrotyrosine, among others, are involved in physiological processes well the mechanisms of response to stress conditions.In sunflower seedlings exposed five different adverse environmental conditions (low temperature, mechanical wounding, high light intensity, continuous light, darkness), key components metabolism reactive nitrogen species (RNS) oxygen (ROS), including enzyme activities L-arginine-...
High temperature (HT) is considered a major abiotic stress that negatively affects both vegetative and reproductive growth. Whereas the metabolism of reactive oxygen species (ROS) well established under HT, less known about nitrogen (RNS). In sunflower (Helianthus annuus L.) seedlings exposed to NO content as S-nitrosoglutathione reductase (GSNOR) activity expression were down-regulated with simultaneous accumulation total S-nitrosothiols (SNOs) including (GSNO). However, tyrosine nitration...
Low temperature (LT) negatively affects plant growth and development via the alteration of metabolism reactive oxygen nitrogen species (ROS RNS). Among RNS, tyrosine nitration, addition an NO2 group to a residue, can modulate reduced nicotinamide-dinucleotide phosphate (NADPH)-generating systems and, therefore, alter levels NADPH, key cofactor in cellular redox homeostasis. NADPH also acts as indispensable electron donor within wide range enzymatic reactions, biosynthetic pathways,...
The antifungal activity of the essential oil aerial parts Bupleurum gibraltarium was evaluated against Plasmopara halstedii. Fungus spores were inoculated in sunflower seedlings, previously treated with several solutions, and sporulation percentage measured after an 11-day treatment. at a concentration 5.0 mL/L clearly inhibited fungus sporulation. contact between sporangia minimized, so it seems that pretreatment could activate defense response seedlings pathogen invasion. main compounds...
Nitric oxide (·NO) has been shown to participate in plant response against pathogen infection; however, less is known of the participation other NO-derived molecules designated as reactive nitrogen species (RNS). Using two sunflower (Helianthus annuus L.) cultivars with different sensitivity infection by Plasmopara halstedii, we studied key components involved RNS and ROS metabolism. We analyzed superoxide radical production, hydrogen peroxide content, l-arginine-dependent nitric synthase...
Abstract Both enzymatic activities increased under B‐deficient and B‐toxic treatments. The ortho‐diphenolic content did not change with B levels. Our results suggest that the primary action on OPP pathway is at first enzyme (glucose‐6P‐dehydrogenase) level effect 6P‐gluconate‐dehydrogenase secondary to this. seems be caused by any direct interaction substrates, as infiltration of culture “in vivo”; result in long term effects cell structures and/or processes easily r In this paper...
Abstract Cataiase, peroxidase and IAA‐oxidase activities, so as odiphenolics content in sunflower leaves grown hydroponics with 4 B levels (0.05; 0.25; 0.5 2.0 ppm) were determined. The biochemical assays made when visual symptons clearly developed. Cataiase activity significantly increased deficient leaves. Peroxidase, activities o‐diphenolics did not change levels. lag period for was reduced toxic respect to the normal ones. These results are accordance hypothesis that one of action could...
Abstract Homogenates were prepared from the leaves of hydroponically cultivated sunflowers (Helianthus annuus, L.) under deficient, normal and toxic B conditions polyphenol oxidase activity together with ortho‐diphenolic micronutrient content was measured every week one month to flowering. deficiency slightly depressed toxicity decreased it significantly at all times, response fitting a line versus logarithm concentration in solution. We found positive correlation between leaves. action on...
Abstract Homogenates were prepared from the leaves of hydroponically cultivated sunflower (Helianthus annuus, L.) under deficient, normal and toxic B conditions phenylalanine ammonia‐lyase activity together with ortho‐diphenolic micronutrient content measured every week one month to flowering The restoration enzymatic on exogenous addition borate “in vivo”; was also studied. Both toxicity deficiency resulted in increased homogenates effect early sustained late transitory. “In vitro”;...