Post-translational modifications (PTMs) mediated by nitric oxide (NO)-derived molecules have become a new area of research, as they can modulate the function target proteins. Proteomic data shown that ascorbate peroxidase (APX) is one potential targets PTMs NO-derived molecules. Using recombinant pea cytosolic APX, impact peroxynitrite (ONOO–) and S-nitrosoglutathione (GSNO), which are known to mediate protein nitration S-nitrosylation processes, respectively, was analysed. While inhibits...

Tyrosine nitration is recognized as an important post-translational protein modification in animal cells that can be used indicator of a nitrosative process. However, plant systems, there scant information on proteins undergo this In sunflower hypocotyls, the content tyrosine (NO(2)-Tyr) and identification nitrated were studied by high-performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) proteomic approaches, respectively. addition, cell localization nitrotyrosine...

The easy functionalization of tags and solid supports with the vinyl sulfone function is a valuable tool in omic sciences that allows their coupling amine thiol groups present proteogenic residues proteins, mild green conditions compatible biological function.

The ascorbate–glutathione cycle is a metabolic pathway that detoxifies hydrogen peroxide and involves enzymatic non-enzymatic antioxidants. Proteomic studies have shown some enzymes in this such as ascorbate peroxidase (APX), monodehydroascorbate reductase (MDAR), glutathione (GR) are potential targets for post-translational modifications (PMTs) mediated by nitric oxide-derived molecules. Using purified recombinant pea peroxisomal MDAR cytosolic chloroplastic GR produced Escherichia coli,...

High temperature (HT) is considered a major abiotic stress that negatively affects both vegetative and reproductive growth. Whereas the metabolism of reactive oxygen species (ROS) well established under HT, less known about nitrogen (RNS). In sunflower (Helianthus annuus L.) seedlings exposed to NO content as S-nitrosoglutathione reductase (GSNOR) activity expression were down-regulated with simultaneous accumulation total S-nitrosothiols (SNOs) including (GSNO). However, tyrosine nitration...

Nitro-fatty acids (NO2-FAs) are novel molecules resulting from the interaction of unsaturated fatty and nitric oxide (NO) or NO-related molecules. In plants, it has recently been described that NO2-FAs trigger a powerful antioxidant defense response against stressful situations, induction heat-shock (HSR), they exert their signaling function mainly through reversible post-translational modification called nitroalkylation. Catalase (CAT) is key enzyme for control hydrogen peroxide (H2O2)...

Abstract Novel supported chelating adsorbents bearing diverse multidentate nitrogenated ligands with strong copper(I) affinities are easily prepared in non‐magnetic and magnetic variants using silica silica‐coated magnetite nanoparticles as suitable supports the aza‐Michael‐type addition of vinyl sulfones ligation tool. These versatile materials applications copper‐catalyzed alkyne‐azide cycloaddition (CuAAC) click chemistry where their complexation abilities enable them to act either...

A new approach for the preparation of carbohydrate-coated magnetic nanoparticles is reported. In a first step, we show that pH-driven assembly-disassembly natural process occurs in apoferritin protein effective encapsulation maghemite different sizes: 4 and 6 nm. second demonstrate presence functional amine groups outer shell allows functionalization with two carbohydrates, N-acetyl-d-glucosamine d-mannose. High-resolution electron microscopy (HREM), high angle annular dark field scanning...

Nitric oxide (NO) is a free radical which modulates protein function and gene expression throughout all stages of plant development. Fruit ripening involves complex scenario where drastic phenotypical metabolic changes take place. Pepper fruits are one the most consumed horticultural products worldwide which, at ripening, undergo crucial biochemical events, with NO antioxidants being implicated. Based on previous transcriptomic (RNA-Seq), proteomics (iTRAQ), enzymatic data, this study aimed...

Aims: Pepper fruit is a horticultural product worldwide consumed that has great nutritional and economic relevance. Besides the phenotypical changes undergo pepper during ripening, there are many associated modifications at transcriptomic, proteomic, biochemical, metabolic levels. Nitric oxide (NO) hydrogen sulfide (H2S) recognized signal molecules can exert regulatory functions in diverse plant processes. This study aims analyzing interrelationship between NO H2S ripening. Results: Our data...

Chloroplastic fructose‐1,6‐bisphosphatases are redox regulatory enzymes which activated by the ferredoxin thioredoxin system via reduction/isomerization of a critical disulfide bridge. All chloroplastic sequences contain seven cysteine residues, four located in, or close to, an amino acid insertion region approximately 17 acids. In order to gain more information on nature site, five residues (Cys 49 , Cys 153 173 178 and 190 ) have been modified individually into serine site‐directed...

Abstract The covalent immobilization of suitable alkyne/azide carbohydrate derivatives on complementarily functionalizated azide/alkyne silica was performed by click ligation througth the Cu(I)‐catalyzed 1,3‐dipolar cycloaddition reaction such compounds. new glyco‐silicas have shown to be efficient and valuable bio‐selective affinity chromatographic supports for purification lectins as well one‐pot fluorescent labeling those proteins. synthetic methodology is simple, high yielding flexible,...

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTSynthesis of Calixarene-Based Cavitands and Nanotubes by Click ChemistryJulia Morales-Sanfrutos, Mariano Ortega-Muñoz, Javier Lopez-Jaramillo, Fernando Hernandez-Mateo, Francisco Santoyo-Gonzalez*View Author Information Instituto de Biotecnología, Departamento Química Orgánica, Facultad Ciencias, Campus Fuentenueva s/n, Universidad Granada, Granada E-18071, Spain[email protected]Cite this: J. Org. Chem. 2008, 73, 19, 7768–7771Publication Date...

The combination of silica as support and vinyl sulfone reactive group led to a pre-activated material that readily reacts form covalent bonds by Michael-type addition with both amine thiol groups naturally occurring in biomolecules mild conditions compatible the biological nature enzymes. A simple two step synthetic strategy was designed access this functionalized hybrid material. Two types silicas (N-type S-type) differing chemical linkers between particle were prepared implementation...

The antioxidant function of 2-Cys peroxiredoxin (Prx) involves the oxidation its conserved peroxidatic cysteine to sulphenic acid that is recycled by a reductor agent. In conditions oxidative stress, can be overoxidized sulphinic inactivating Prx. An enzyme recently discovered, named sulfiredoxin (Srx), reduces Prx (Prx-SO2H). To explore physiological functions Srx in plants we have cloned, expressed and purified homogeneity from Arabidopsis thaliana (AtSrx), as well five variants...

The aim of this study was to evaluate the crosslinking abilities divinyl sulfone (DVS) for preparation novel water-insoluble cyclodextrin-based polymers (CDPs) capable forming inclusion complexes with different guest molecules. Reaction DVS native α-cyclodextrin (α-CD), β-cyclodextrin (β-CD) and/or starch generates a variety homo- and hetero-CDPs degrees as function reactants’ stoichiometric ratio. materials were characterized by powder X-ray diffraction, electron microscopy their sorption...

Abstract Background S -nitrosylaton is implicated in the regulation of numerous signaling pathways with a diversity regulatory roles. The high lability S-NO bond makes study proteins regulated by S-nitrosylation/denitrosylation challenging task and most studies have focused on already S-nitrosylated proteins. We hypothesize that: i) S-nitrosoglutathione (GSNO) transnitrosylation feasible mechanism to account for physiological S-nitrosylation rather electropositive sulfur atoms from proteins,...

The introduction of multiple labels onto biomolecules is a challenge. We report herein the synthesis vinyl sulfone derivatized bifunctional tag single-attachment-point reagents (BTSAP) bearing biotin and fluorescent their applications in proteins for by means Michael-type addition electrophilic group. These BTSAP were easily synthesized two-step chemical strategy involving preparation alkyne tags (AVST) subsequent click CuAAC attachment second azide functionalized tag. direct coupling with...

Carbohydrate-mediated molecular recognition is involved in many biological aspects such as cellular adhesion, immune response, blood coagulation, inflammation, and infection. Considering the crucial importance of events which proteins are normally involved, synthetic saccharide-based systems have emerged powerful tools for understanding protein-carbohydrate interactions. As a new approach to create systems, set representative monosaccharides (D-mannose, D-glucose, N-acetyl-D-glucosamine,...