[reaction: see text] The construction of multivalent neoglycoconjugates is efficiently achieved by the regiospecific catalytic cycloaddition alkynes and azides using organic-soluble copper complexes (Ph(3)P)(3).CuBr (EtO)(3)P.CuI. simultaneous use microwave irradiation shortened notably reaction times.

The easy functionalization of tags and solid supports with the vinyl sulfone function is a valuable tool in omic sciences that allows their coupling amine thiol groups present proteogenic residues proteins, mild green conditions compatible biological function.

The use of edible coatings has surged as a response to the ever-increasing demand for ecologically-friendly methods maintaining fruit quality during storage. This study analyses application different polysaccharide-based coatings, carboxymethylcellulose, chitosan, dextrin and starch, plasticizer agent, glycerol, with shown be most effective in postharvest zucchini cold Subsequently, improve these results, nutraceutical additives oleuropein olive oil, were tested combination dextrin. Results...

Abstract Novel supported chelating adsorbents bearing diverse multidentate nitrogenated ligands with strong copper(I) affinities are easily prepared in non‐magnetic and magnetic variants using silica silica‐coated magnetite nanoparticles as suitable supports the aza‐Michael‐type addition of vinyl sulfones ligation tool. These versatile materials applications copper‐catalyzed alkyne‐azide cycloaddition (CuAAC) click chemistry where their complexation abilities enable them to act either...

A new approach for the preparation of carbohydrate-coated magnetic nanoparticles is reported. In a first step, we show that pH-driven assembly-disassembly natural process occurs in apoferritin protein effective encapsulation maghemite different sizes: 4 and 6 nm. second demonstrate presence functional amine groups outer shell allows functionalization with two carbohydrates, N-acetyl-d-glucosamine d-mannose. High-resolution electron microscopy (HREM), high angle annular dark field scanning...

Abstract Protein engineering studies often suggest the emergence of completely new enzyme functionalities to be highly improbable. However, enzymes likely catalysed many different reactions already in last universal common ancestor. Mechanisms for active sites must therefore either plausibly exist or at least have existed primordial protein stage. Here, we use resurrected Precambrian proteins as scaffolds and demonstrate that a site can generated through single hydrophobic-to-ionizable amino...

Abstract The covalent immobilization of suitable alkyne/azide carbohydrate derivatives on complementarily functionalizated azide/alkyne silica was performed by click ligation througth the Cu(I)‐catalyzed 1,3‐dipolar cycloaddition reaction such compounds. new glyco‐silicas have shown to be efficient and valuable bio‐selective affinity chromatographic supports for purification lectins as well one‐pot fluorescent labeling those proteins. synthetic methodology is simple, high yielding flexible,...

Salivary pH serves as a valuable and useful diagnostic marker for periodontal disease, it not only plays critical role in disease prevention but also its development. Typically, saliva sampling is collected by draining spitting into collection tubes or using swabs. In this study, we have developed Point-of-Care (POC) device situ determination of oral without the need complex instruments, relying solely on smartphone detection device. Our system utilizes non-toxic vegetable colourimetric...

Enzymes are the quintessential green catalysts, but realizing their full potential for biotechnology typically requires improvement of biomolecular properties. Catalysis enhancement, however, is often accompanied by impaired stability. Here, we show how interplay between activity and stability in enzyme optimization can be efficiently addressed coupling two recently proposed methodologies guiding directed evolution. We first identify catalytic hotspots from chemical shift perturbations...

The efficient synthesis of fluorescent and non-fluorescent multivalent neoglycoconjugates is described by means the Cu(I) catalyzed azide–alkyne 1,3-dipolar cycloaddition ("click-chemistry"). A well-defined glycopolymer, glycocyclodextrin or glycocluster architecture displaying galactose lactose epitopes has been chosen. Cellular assays using U-937 RAW 264.7 monocyte/macrophage cells showed that these glycocompounds have capability to act as synthetic activators mimicking lipopolysaccharide...

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTSynthesis of Calixarene-Based Cavitands and Nanotubes by Click ChemistryJulia Morales-Sanfrutos, Mariano Ortega-Muñoz, Javier Lopez-Jaramillo, Fernando Hernandez-Mateo, Francisco Santoyo-Gonzalez*View Author Information Instituto de Biotecnología, Departamento Química Orgánica, Facultad Ciencias, Campus Fuentenueva s/n, Universidad Granada, Granada E-18071, Spain[email protected]Cite this: J. Org. Chem. 2008, 73, 19, 7768–7771Publication Date...

Abstract The efficient synthesis of structurally diverse, multivalent, heterogeneous neoglycoconjugates by means a series different click‐based strategies is described. methodology highly and versatile allowing for easy access to mannose (α‐Man)‐containing glycoconjugates differing in their valency, nature the scaffold, constitutive sugars length linker. influence those structural parameters on binding affinities these glycomimics toward Concanavalin A (Con A) was evaluated.(© Wiley‐VCH...

The combination of silica as support and vinyl sulfone reactive group led to a pre-activated material that readily reacts form covalent bonds by Michael-type addition with both amine thiol groups naturally occurring in biomolecules mild conditions compatible the biological nature enzymes. A simple two step synthetic strategy was designed access this functionalized hybrid material. Two types silicas (N-type S-type) differing chemical linkers between particle were prepared implementation...

Abstract Background S -nitrosylaton is implicated in the regulation of numerous signaling pathways with a diversity regulatory roles. The high lability S-NO bond makes study proteins regulated by S-nitrosylation/denitrosylation challenging task and most studies have focused on already S-nitrosylated proteins. We hypothesize that: i) S-nitrosoglutathione (GSNO) transnitrosylation feasible mechanism to account for physiological S-nitrosylation rather electropositive sulfur atoms from proteins,...

Directed evolution has revolutionized protein engineering. Still, enzyme optimization by random library screening remains sluggish, in large part due to futile probing of mutations that are catalytically neutral and/or impair stability and folding. FuncLib is a novel approach which uses phylogenetic analysis Rosetta design rank variants with multiple mutations, on the basis predicted stability. Here, we use it target active site region minimalist-designed, de novo Kemp eliminase. The...

Carbohydrate-mediated molecular recognition is involved in many biological aspects such as cellular adhesion, immune response, blood coagulation, inflammation, and infection. Considering the crucial importance of events which proteins are normally involved, synthetic saccharide-based systems have emerged powerful tools for understanding protein-carbohydrate interactions. As a new approach to create systems, set representative monosaccharides (D-mannose, D-glucose, N-acetyl-D-glucosamine,...

Multimeric lactosides based on carbohydrate scaffolds with valencies ranging from 1 to 4 and different linker lengths were synthesized by a copper-catalyzed azide-alkyne cycloaddition (CuAAC). The binding affinities crosslinking abilities of the new "click clusters" toward biologically relevant galectins (gal-1, gal-3) peanut lectin evaluated fluorescent polarization assay (FPA) enzyme-linked (ELLA), respectively. FPA indicated that synthetic multilactosides towards increased proportionally...

Gene transfection mediated by the cationic polymer polyethylenimine (PEI) is considered a standard methodology. However, while highly branched PEIs form smaller polyplexes with DNA that exhibit high efficiencies, they have significant cell toxicity. Conversely, low molecular weight (LMW-PEIs) favorable cytotoxicity profiles display minimum activities as result of inadequate complexation and protection. To solve this paradox, novel polyelectrolyte complex was prepared ionic cross-linking 1.8...

Vinyl sulfone-silica was efficient to covalently immobilize levansucrase at neutral pH. LEV-VS showed altered selectivity towards FOS and good operational stability.

A microfluidic paper-based analytical device integrating a chromoreactand – formylazo dye– has been fabricated and used for colorimetric assay of sulfites. The was covalently linked to paper by vinyl sulfone chemistry. This work presents two robust capillary devices determine sulfite in wine without any pretreatment. One them based on thread (µTPAD) useful it white another (µPAD) specifically red as well wine. Both are the selective recognition means that turns from orange yellow presence...

The covalent synthesis of nanosized cage compounds is easily performed in high yields using "click chemistry" methodology through the Cu(I)-catalyzed ligation adequate polyalkyne and polyazide derivatives (EtO)3P x CuI as catalyst.