A5091822703- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme-mediated dye degradation
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolism and Applications
- Studies on Chitinases and Chitosanases
- Digestive system and related health
- Analytical Chemistry and Chromatography
- Electrochemical sensors and biosensors
- Protein Hydrolysis and Bioactive Peptides
- Antioxidant Activity and Oxidative Stress
- Nanocomposite Films for Food Packaging
- Diet, Metabolism, and Disease
- Organometallic Complex Synthesis and Catalysis
- Phytochemicals and Antioxidant Activities
- Phytase and its Applications
- Glycosylation and Glycoproteins Research
- Infant Nutrition and Health
- Biochemical and biochemical processes
- Free Radicals and Antioxidants
- Vitamin C and Antioxidants Research
- Metal complexes synthesis and properties
Instituto de Catálisis y Petroleoquímica
2015-2024
Consejo Superior de Investigaciones Científicas
2012-2023
Marie Curie
2022
Universidad de Zaragoza
1988-2019
Unidades Centrales Científico-Técnicas
2013
Microsystems (United Kingdom)
2013
Universidad Autónoma de Madrid
1999-2012
Universidad Politécnica de Madrid
2012
Instituto de Ciencia de Materiales de Sevilla
2012
Air Products (Spain)
2012
Laccases are multi-copper containing oxidases (EC 1.10.3.2), widely distributed in fungi, higher plants and bacteria. Laccase catalyses the oxidation of phenols, polyphenols anilines by one-electron abstraction, with concomitant reduction oxygen to water a four-electron transfer process. In presence small redox mediators, laccase offers broader repertory oxidations including non-phenolic substrates. Hence, fungal laccases considered as ideal green catalysts great biotechnological impact due...
Hydrogen peroxide (H2O2) is a compound involved in some mammalian reactions and processes. It modulates signals the redox metabolism of cells by acting as messenger together with hydrogen sulfide (H2S) nitric oxide radical (•NO), activating specific oxidations that determine metabolic response. The reaction triggered determines cell survival or apoptosis, depending on which downstream pathways are activated. There several ways to produce H2O2 cells, cellular systems tightly control its...
In this review, we have focused mainly on the study of their antioxidant and pro-oxidant capacity, analysis oxidation catechol group to o-quinone. The redox balance established between different oxidase reductase enzymes generates reducing species, H+ e−, allows polyphenolic groups quinones be reversible. This continuous these nucleophilic electrophilic substances activation NRF2/ARE axis, which regulates cellular responses against oxidative stress, as well cell proliferation. Understanding...
Free radicals (FRs) are unstable molecules that cause reactive stress (RS), an imbalance between oxygen and nitrogen species in the body its ability to neutralize them. These generated by both internal external factors can damage cellular lipids, proteins, DNA. Antioxidants prevent or slow down oxidation process interrupting transfer of electrons substances agents. This is particularly important at level because reactions lead formation FR contribute various diseases. As we age, RS...
RL5, a gene coding for novel polyphenol oxidase, was identified through activity screening of metagenome expression library from bovine rumen microflora. Characterization the recombinant protein produced in Escherichia coli revealed multipotent capacity to oxidize wide range substrates (syringaldazine > 2,6-dimethoxyphenol veratryl alcohol guaiacol tetramethylbenzidine 4-methoxybenzyl 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) >> phenol red) over an unusually broad pH 3.5...
Abstract In the last decade, new trends for enzyme attachment to solid carriers have emerged in an attempt rationalize classical methods immobilization. silico analysis is becoming a powerful tool predict orientation of enzymes covalently attached carrier or protein regions involved adsorption support. Significantly, array algorithms has been established rational design immobilized derivatives, which comprises both size and textural properties Ordered mesoporous materials open challenging...
Efficient transformation of several polycyclic aromatic hydrocarbons (PAHs) was obtained using a fungal laccase in the presence phenolic compounds related to those formed nature during turnover lignin and humus. The effect these natural mediators, namely vanillin, acetovanillone, acetosyringone, syringaldehyde, 2,4,6-trimethylphenol, p-coumaric acid, ferulic sinapic compared with that synthetic mediators such as 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) 1-hydroxybenzotriazole...
The potential of lipases (E.C.3.1.1.3.) as biocatalysts for the production fatty acid derivatives food and nutraceutical industries, such flavouring esters, esters antioxidants structured lipids, is enormous, mainly due to their high regio-and stereo-selectivities, in addition other well-known advantages enzymatic processes.The replacement chemical catalysts by presents great benefits terms nutritional properties obtained products environmental care.The reactions performed these compounds,...
The synthesis of galactooligosaccharides (GOS) catalyzed by β-galactosidase from Aspergillus oryzae (Enzeco) was studied. Using 400 g/L lactose and 15 U/mL, maximum GOS yield, measured HPAEC-PAD, 26.8% w/w total carbohydrates, obtained at approximately 70% conversion. No less than 17 carbohydrates were identified; the major transgalactosylation product 6′-O-β-galactosyl-lactose, representing nearly one-third (in weight) GOS. In contrast with previous reports, presence least five...
Abstract There is an increasing interest in enzymes that catalyze the hydroxylation of naphthalene under mild conditions and with minimal requirements. To address this challenge, extracellular fungal aromatic peroxygenase mono(per)oxygenase activity was engineered to convert selectively into 1‐naphthol. Mutant libraries constructed by random mutagenesis DNA recombination were screened for on together quenching undesired peroxidative 1‐naphthol (one‐electron oxidation). The resulting double...
In recent years, much interest has been generated by the idea that nitrosative stress plays a role in aetiology of human diseases, such as atherosclerosis, inflammation, cancer, and neurological diseases. The chemical changes mediated reactive nitrogen species (RNS) are detrimental to cell function, because they can cause nitration, which alter structures cellular proteins, DNA, lipids, hence, impair their normal function. One most potent biological agents is peroxynitrite (ONOO−), produced...