A5073599071- Microbial Community Ecology and Physiology
- Microbial bioremediation and biosurfactants
- Genomics and Phylogenetic Studies
- Semiconductor materials and devices
- Semiconductor Quantum Structures and Devices
- Methane Hydrates and Related Phenomena
- Advancements in Semiconductor Devices and Circuit Design
- Semiconductor Lasers and Optical Devices
- Microbial Metabolic Engineering and Bioproduction
- Advanced Semiconductor Detectors and Materials
- Protist diversity and phylogeny
- Enzyme Catalysis and Immobilization
- Photonic and Optical Devices
- Quantum Dots Synthesis And Properties
- Semiconductor materials and interfaces
- Integrated Circuits and Semiconductor Failure Analysis
- Marine and coastal ecosystems
- Biofuel production and bioconversion
- Hydrocarbon exploration and reservoir analysis
- Oil Spill Detection and Mitigation
- Enzyme Production and Characterization
- Metal Extraction and Bioleaching
- Electronic and Structural Properties of Oxides
- Nanowire Synthesis and Applications
- Wastewater Treatment and Nitrogen Removal
National Research Council
2009-2024
University at Albany, State University of New York
2008-2024
Albany State University
2000-2024
SUNY Polytechnic Institute
2016-2023
Institute for Coastal Marine Environment
2013-2022
Manhattan College
2022
Stony Brook University
2022
IBM Research - Thomas J. Watson Research Center
2022
Brookhaven National Laboratory
2022
Institute for Marine Biological Resources and Biotechnology
2021
During screening for biosurfactant-producing, n-alkane-degrading marine bacteria, six heterotrophic bacterial strains were isolated from enriched mixed cultures, obtained sea water/sediment samples collected near the Isle of Borkum (North Sea), using Mihagol-S (C14,15-n-alkanes) as principal carbon source. These Gram-negative, aerobic, rod-shaped bacteria use a limited number organic compounds, including aliphatic hydrocarbons, volatile fatty acids, and pyruvate its methyl ether. cultivation...
Strain BAS50, isolated from a petroleum reservoir at depth of 1,500 m and identified as Bacillus licheniformis, grew produced lipopeptide surfactant when cultured on variety substrates salinities up to 13% NaCl. Surfactant production occurred both aerobically anaerobically was optimal 5% NaCl temperatures between 35 45 degrees C. The biosurfactant, termed lichenysin A, purified chemically characterized. A tentative structure composition for the are described. Lichenysin is mixture...
Alcanivorax borkumensis is a cosmopolitan marine bacterium that uses oil hydrocarbons as its exclusive source of carbon and energy. Although barely detectable in unpolluted environments, A. becomes the dominant microbe oil-polluted waters. SK2 has streamlined genome with paucity mobile genetic elements energy generation–related genes, but plethora genes accounting for wide hydrocarbon substrate range efficient oil-degradation capabilities. The further specifies systems scavenging nutrients,...
Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of marine microbial biodiversity and function world's oceans. It is simultaneous global mega-sequencing campaign aiming generate largest standardized data set in single day. This will be achievable only through coordinated efforts an Consortium, supportive partnerships networks between sites. commentary outlines establishment, aims Consortium...
Practical experiments drive important scientific discoveries in biology, but theory-based research studies also contribute novel-sometimes paradigm-changing-findings. Here, we appraise the roles of approaches focusing on experiment-dominated wet-biology areas microbial growth and survival, cell physiology, host-pathogen interactions, competitive or symbiotic interactions. Additional examples relate to analyses genome-sequence data, climate change planetary health, habitability, astrobiology....
An isolate of an acidophilic archaeon, strain YT, was obtained from a bioleaching pilot plant. The organism oxidizes ferrous iron as the sole energy source and fixes inorganic carbon source. optimal pH for growth is 1.7, although observed in range 1.3 to 2.2. cells are pleomorphic without cell wall. 16S rRNA gene sequence analysis showed this cluster phylogenetically within order 'Thermoplasmales' sensu Woese, with only 89.9 87.2% identity, respectively, its closest relatives,...
A metagenome expression library of bulk DNA extracted from the rumen content a dairy cow was established in phage lambda vector and activity-based screening employed to explore functional diversity microbial flora. Twenty-two clones specifying distinct hydrolytic activities (12 esterases, nine endo-beta-1,4-glucanases one cyclodextrinase) were identified characterized. Sequence analysis retrieved enzymes revealed that eight (36%) entirely new formed deep-branched phylogenetic lineages with...
The taxonomic characteristics of two bacterial strains, RB-8T and RB-9, isolated from hydrocarbon-degrading enrichment cultures obtained Antarctic coastal marine environments (Rod Bay, Ross Sea), were determined. These bacteria psychrophilic, aerobic Gram-negative with polar flagella. Growth was not observed in the absence NaCl, occurred only at concentrations Na+ above 20 mM optimal an NaCl concentration 3–5 % (w/v). major cellular fatty acids monounsaturated straight-chain acids. strains...
The biosphere of planet Earth is delineated by physico-chemical conditions that are too harsh for, or inconsistent with, life processes and maintenance the structure function biomolecules. To define window on (and perhaps gain insights into limits could tolerate elsewhere), hence understand some most unusual biological activities operate at such extremes, it necessary to causes cellular basis systems failure beyond these windows. Because water plays a central role in biomolecules...
Summary Hydrocarbon‐contaminated superficial sediments collected from the Harbor of Milazzo (Tirrenean Sea, northern Sicily), a zone strongly affected by anthropogenic activities, were examined for in situ biodegradative capacities. A culture‐independent molecular phylogenetic approach was used to study influence hydrocarbon and nutrient addition on activity diversity indigenous microbiota during microcosm evaluation. The autochthonous microbial community non‐polluted represented eubacterial...
We demonstrate the electrical properties of metal-oxide-semiconductor capacitors on molecular beam epitaxial GaAs in situ passivated with ultrathin amorphous Si (a-Si) layer and ex deposited HfO2 gate oxide TaN metal gate. Minimum thickness interface passivation 1.5 nm is needed to prevent Fermi level pinning provide good capacitance-voltage characteristics equivalent 2.1 leakage current ⩽1.0mA∕cm2. Transmission electron microscopy analysis showed that was oxidized up 1.4 during processing...
RL5, a gene coding for novel polyphenol oxidase, was identified through activity screening of metagenome expression library from bovine rumen microflora. Characterization the recombinant protein produced in Escherichia coli revealed multipotent capacity to oxidize wide range substrates (syringaldazine > 2,6-dimethoxyphenol veratryl alcohol guaiacol tetramethylbenzidine 4-methoxybenzyl 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) >> phenol red) over an unusually broad pH 3.5...
Summary There is little information on how different strategies for the bioremediation of marine oil spills influence key indigenous hydrocarbon‐degrading bacteria (hydrocarbonoclastic bacteria, HCB), and hence their remediation efficacy. Therefore, we have used quantitative polymerase chain reaction to analyse changes in concentrations HCB response intervention applied experimental microcosms. Biostimulation with nutrients (N P) produced no measurable increase either biodegradation or...
Microcosm experiments simulating an oil spill event were performed to evaluate the response of natural microbial community structure Messina harbour seawater following accidental load petroleum.An experimental microcosm, supplemented with nutrients and crude oil, was monitored above 15 days in comparison unpolluted ones (control microcosms). Bacterial cells counted a Live/Dead BacLight viability kit; leucine aminopeptidase, beta-glucosidase, alkaline phosphatase, lipase esterase enzymes...
The frontiers of eukaryote life in nature are still unidentified. In this study, we analysed protistan communities the hypersaline (up to 365 g l(-1) NaCl) anoxic L'Atalante deep-sea basin located eastern Mediterranean Sea. Targeting 18S ribosomal RNA retrieved from basin's lower halocline (3501 m depth) detected 279 sequences that grouped into 42 unique phylotypes (99% sequence similarity). Statistical analyses revealed these account only for a proportion protists inhabiting harsh...
Ubiquitous bacteria from the genus Oleispira drive oil degradation in largest environment on Earth, cold and deep sea. Here we report genome sequence of antarctica show that compared with Alcanivorax borkumensis—the paradigm mesophilic hydrocarbonoclastic bacteria—O. has a larger witnessed massive gene-transfer events. We identify an array alkane monooxygenases, osmoprotectants, siderophores micronutrient-scavenging pathways. also at low temperatures, main protein-folding machine Cpn60...
Esterases receive special attention because of their wide distribution in biological systems and environments importance for physiology chemical synthesis. The prediction esterases' substrate promiscuity level from sequence data the molecular reasons why certain such enzymes are more promiscuous than others remain to be elucidated. This limits surveillance space esterases potentially leading new versatile biocatalysts insights into role cellular function. Here, we performed an extensive...
Within the complex of deep, hypersaline anoxic lakes (DHALs) Mediterranean Ridge, we identified a new, unexplored DHAL and named it 'Lake Kryos' after nearby depression. This lake is filled with magnesium chloride (MgCl2 )-rich, athalassohaline brine (salinity > 470 practical salinity units), presumably formed by dissolution Messinian bischofite. Compared Discovery, contains elevated concentrations kosmotropic sodium sulfate ions, which are capable reducing net chaotropicily MgCl2 -rich...
Abstract Intriguing, yet uncultured ‘ARMAN’-like archaea are metabolically dependent on other members of the microbial community. It remains uncertain though which hosts they rely upon, and, because lack complete genomes, to what extent. Here, we report co-culturing ARMAN-2-related organism, Mia14, with Cuniculiplasma divulgatum PM4 during isolation this strain from acidic streamer in Parys Mountain (Isle Anglesey, UK). Mia14 is highly enriched binary culture (ca. 10% genomic reads) and its...