A5025237070- Metal Extraction and Bioleaching
- Mine drainage and remediation techniques
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Marine Biology and Ecology Research
- Enzyme Catalysis and Immobilization
- Protein Hydrolysis and Bioactive Peptides
- Enzyme Structure and Function
- Minerals Flotation and Separation Techniques
- Enzyme Production and Characterization
- Paleontology and Stratigraphy of Fossils
- Mineral Processing and Grinding
- Microbial Metabolic Engineering and Bioproduction
- Polyamine Metabolism and Applications
- Planetary Science and Exploration
- Geology and Paleoclimatology Research
- Protein purification and stability
- Plant biochemistry and biosynthesis
- Methane Hydrates and Related Phenomena
- Ubiquitin and proteasome pathways
- Collagen: Extraction and Characterization
- Advanced Proteomics Techniques and Applications
- Biochemical and Molecular Research
- Phytase and its Applications
- Bacteriophages and microbial interactions
NORCE Norwegian Research Centre
2018-2021
University of Bergen
2011-2020
Uni Research (Norway)
2017-2019
University of the Free State
2011-2014
Centro de Biología Molecular Severo Ochoa
2006-2012
Universidad Autónoma de Madrid
2006-2012
Esterases receive special attention because of their wide distribution in biological systems and environments importance for physiology chemical synthesis. The prediction esterases' substrate promiscuity level from sequence data the molecular reasons why certain such enzymes are more promiscuous than others remain to be elucidated. This limits surveillance space esterases potentially leading new versatile biocatalysts insights into role cellular function. Here, we performed an extensive...
Due to its highly metalliferous waters and low pH, the Rio Tinto has shown potential for modelling both acid mine drainage systems biohydrometallurgical operations. Most geomicrobiological studies of these have focused on oxic water column. A sequence-based approach in combination with situ detection techniques enabled us examine composition structure microbial communities associated suboxic anoxic sediments along river course compare them planktonic inhabiting The results obtained different...
The growing concern over the transmission of pathogens, particularly in high-risk environments such as healthcare facilities and public spaces, necessitates development effective sustainable antimicrobial solutions. Traditional coatings often rely on metals, which despite their efficacy, pose significant environmental economic challenges. This study explores potential bio-based alkyd resins, supplemented with natural bioadditives, an eco-friendly alternative to traditional antibacterial...
Svalbard, situated in the high Arctic, is an important past and present coal mining area. Dozens of abandoned waste rock piles can be found proximity Longyearbyen. This environment offers a unique opportunity for studying biological control over weathering sulphide rocks at low temperatures. Although extension impact acid mine drainage (AMD) this area known, native microbial communities involved process are still scarcely studied uncharacterized. Several areas were explored search active AMD...
Summary An oligonucleotide microarray that monitors prokaryotic diversity in extremely acidic environments has been developed. The probes target most known acidophilic microorganisms, including members of the Nitrospira phylum, Acidithiobacillus genus, acidobacteria, sulfur reducing bacteria, Actinobacteria and Archaea Ferroplasma Thermoplasma genera. were tested for their specificity against corresponding type strain by hybridization using PCR‐amplified fluorescent DNA 16S rRNA genes. was...
In the South African deep mines, a variety of biofilms growing in mine corridor walls as water seeps from intersections or fractures represents excellent proxies for deep-subsurface environments. However, they may be greatly affected by oxygen inputs through galleries mining activities. As consequence, interaction between anaerobic coming out with creates new conditions that support rich microbial communities. The inherent difficulties sampling these delicate habitats, together transport and...
Abstract Eukarya have been discovered in the deep subsurface at several locations South Africa, but how organisms reach remains unknown. We studied river-subsurface fissure water systems and identified from a river that are genetically identical for 18S rDNA. To further confirm these species one metazoan recovered overlying interbred successfully with specimen an underlying mine −1.4 km. In situ seismic simulation experiments were carried out show activity to be major force increasing...
Substrate specificity and selectivity of a biocatalyst are determined by the protein sequence structure its active site. Finding versatile biocatalysts acting against multiple substrates while at same time being chiral selective is interest for pharmaceutical chemical industry. However, relationships between these two properties in natural microbial enzymes remain underexplored. Here, we performed an experimental analysis substrate promiscuity set 145 purified esterases from phylogenetically...
Amination of bulky ketones, particularly in (R) configuration, is an attractive chemical conversion; however, known ω-transaminases (ω-TAs) show insufficient levels performance. By applying two screening methods, we discovered 10 amine transaminases from the class III ω-TA family that were 38% to 76% identical homologues. We present examples such enzymes preferring ketones over keto acids and aldehydes with stringent (S) selectivity. also report representatives ω-TAs capable converting...
Aerobic moderately thermophilic and methane-oxidizing bacteria make a substantial contribution in the control of global warming through biological reduction methane emissions have unique capability utilizing as their sole carbon energy source. Here, we report novel Methylococcus-like Type Ib methanotroph recovered from an alkaline thermal spring (55.4 °C pH 8.82) Ethiopian Rift Valley. The isolate, designated LS7-MC, most probably represents species new genus family Methylococcaceae class...
Since its discovery as part of the bacterial adaptative immune system, CRISPR/Cas has emerged most promising tool for targeted genome editing over past few years. Various tools in Bacillus subtilis have recently been developed, expanding and simplifying potential development an industrial species. A collection vectors compatible with high-throughput (HTP) fragment exchange (FX) cloning heterologous expression Escherichia coli was previously developed. This vector catalogue through this work...
The marine bone biome is a complex assemblage of macro- and microorganisms; however, the enzymatic repertoire to access bone-derived nutrients remains unknown. matrix composite material made up mainly organic collagen inorganic hydroxyapatite. We conducted field experiments study microbial assemblages that can use components as nutrient source. Bovine turkey bones were deposited at 69 m depth in Norwegian fjord (Byfjorden, Bergen). Metagenomic sequence analysis was used assess functional...
Enzyme-based conversion of marine biomass to high-quality peptide ingredients leaves a distinct smell “fish” caused by the presence trimethylamine, which limits their economic potential. We suggest an enzymatic solution for converting trimethylamine odorless N -oxide as novel strategy improve quality protein hydrolysates. Following systematic investigation 45 putative bacterial monooxygenases from several phyla, we expand repertoire known active monooxygenases. As proof-of-concept,...
Abstract To support the bio-based industry in development of environment-friendly processes and products, an optimal toolbox biocatalysts is key. Although functional screen (meta)genomic libraries may potentially contribute to identifying new enzymes, discovery enzymes meeting compliance demands still challenging. This particularly noticeable case proteases, for which reports metagenome-derived proteases with industrial applicability are surprisingly limited. Indeed, proteolytic clones have...
Rio Tinto, a natural extreme acidic environment with rather constant pH and high concentration of heavy metals, is the product metabolic activity chemolithotrophic microorganisms thriving in rich complex sulfides Iberian Pyritic Belt. Up to now microbial characterization has been made mainly water column biofilm samples. Since all ferric reducing activities will not benefit bioleaching operations, we consider it critical ascertain ecology properties inhabiting anoxic part sediments,...
Rio Tinto (Iberian Pyritic Belt, SW Spain) is a natural extreme acidic environment with rather constant pH (mean value 2.3) and high concentration of heavy metals. The ecosystem under the control iron. geomicrobiological characterization Río has unravelled some basic questions biohydrometallurgical interest. methodologies developed for this study were applied successfully to monitor different bioleaching processes BioMinE project.