A5037660495- Peptidase Inhibition and Analysis
- Protein Hydrolysis and Bioactive Peptides
- Signaling Pathways in Disease
- Ubiquitin and proteasome pathways
- Enzyme Catalysis and Immobilization
- Biochemical Analysis and Sensing Techniques
- Polyamine Metabolism and Applications
- Protease and Inhibitor Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- interferon and immune responses
- Cardiac Structural Anomalies and Repair
- Antimicrobial Peptides and Activities
- Cellular transport and secretion
- S100 Proteins and Annexins
- Biotin and Related Studies
- Amino Acid Enzymes and Metabolism
- Aquaculture Nutrition and Growth
- Biochemical and Molecular Research
- PI3K/AKT/mTOR signaling in cancer
- Protein Kinase Regulation and GTPase Signaling
- Protein Degradation and Inhibitors
University of Bergen
2015-2024
NORCE Norwegian Research Centre
2019-2023
Cancer Research Institute
2018
University of Pennsylvania
2018
Highlights d Naa60 is an organelle N-terminal acetyltransferase, and it acts on the cytosolic face Most transmembrane proteins are Nt-acetylated, specifically these mainly localizes to Golgi essential for ribbon structure PROMPT, a novel assay membrane topology of proteins, presented
N-terminal (Nt) acetylation is a major protein modification catalyzed by acetyltransferases (NATs). Methionine acidic N termini, including actin, are cotranslationally Nt acetylated NatB in all eukaryotes, but animal actins containing additionally posttranslationally NAA80. Actin was found to regulate cytoskeletal dynamics and motility, thus making NAA80 potential target for cell migration regulation. In this work, we developed potent selective bisubstrate inhibitors determined the crystal...
About 80% of human proteins are amino-terminally acetylated (Nt-acetylated) by one seven Nt-acetyltransferases (NATs). Actin, the most abundant protein in cytoplasm, has its own dedicated NAT, NAA80, which acts posttranslationally and affects cytoskeleton assembly cell motility. Here, we show that NAA80 does not associate with filamentous actin cells, natural substrate is monomeric actin-profilin complex, consistent Nt-acetylation preceding polymerization. Nt-acetylates much more efficiently...
Nα-Acetyltransferase 60 (Naa60 or NatF) was recently identified as an unconventional N-terminal acetyltransferase (NAT) because it localizes to organelles, in particular the Golgi apparatus, and has a preference for acetylating N termini of transmembrane proteins. This knowledge challenged prevailing view acetylation co-translational ribosome-associated process suggested new mechanistic functioning enzymes responsible this increasingly recognized protein modification. Crystallography studies...
Protein hydrolysates made from marine by-products are very nutritious but frequently contain trimethylamine (TMA), which has an unattractive fish-like smell. Bacterial monooxygenases can oxidize TMA into the odorless N-oxide (TMAO) and have been shown to reduce levels in a salmon protein hydrolysate. To make flavin-containing monooxygenase (FMO) Methylophaga aminisulfidivorans (mFMO) more suitable for industrial application, we engineered it using Repair One-Stop Shop (PROSS) algorithm. All...
Enzyme-based conversion of marine biomass to high-quality peptide ingredients leaves a distinct smell “fish” caused by the presence trimethylamine, which limits their economic potential. We suggest an enzymatic solution for converting trimethylamine odorless N -oxide as novel strategy improve quality protein hydrolysates. Following systematic investigation 45 putative bacterial monooxygenases from several phyla, we expand repertoire known active monooxygenases. As proof-of-concept,...
Besides altering its own expression during cell transformation, Annexin A2 is upregulated the progression of many cancer types and also plays key roles viral infection multiplication. Consequently, there has been great interest in as a potential drug target. The successful design efficient vivo delivery systems constitutes an obstacle full exploitation antisense RNA-cleaving technologies for knock-down specific targets. Efficiency dependent on method accessibility Here, hairpin ribozymes RNA...
AKT is an essential player in the phosphoinositide 3-kinase (PI3K) signalling pathway. Although mechanisms of its action are well understood at plasma membrane, can also be found nucleus. In adipocytes, this pathway activated during process adipogenesis and solicits both membrane nuclear activity. However, endogenous presence active nucleus has not been shown. Here, we show that levels phosphorylated Ser-473 increase rapidly after induction differentiation 3T3-L1 cells, cytoplasm nucleus,...
Abstract Protein hydrolysates made from marine by-products are very nutritious, but frequently contain trimethylamine (TMA) which has an unattractive fish-like smell. Bacterial monooxygenases can oxidize TMA into the odorless N -oxide (TMAO) and have been shown to reduce TMA-levels in a salmon protein hydrolysate. To make Methylophaga aminisulfidivorans monooxygenase, mFMO, more suitable for industrial application, we engineered it using Repair One-Stop Shop (PROSS) algorithm. All seven...