A5038393536- S100 Proteins and Annexins
- RNA Research and Splicing
- Heat shock proteins research
- RNA and protein synthesis mechanisms
- Protease and Inhibitor Mechanisms
- Computational Drug Discovery Methods
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Nuclear Structure and Function
- Traumatic Brain Injury and Neurovascular Disturbances
- RNA Interference and Gene Delivery
- Mitochondrial Function and Pathology
- Cellular Mechanics and Interactions
- Lysosomal Storage Disorders Research
- Lipid metabolism and biosynthesis
- RNA regulation and disease
- Microtubule and mitosis dynamics
- Microbial Inactivation Methods
- Sesquiterpenes and Asteraceae Studies
- Metabolism, Diabetes, and Cancer
- Protein Structure and Dynamics
- Immune Response and Inflammation
- Endoplasmic Reticulum Stress and Disease
- Erythrocyte Function and Pathophysiology
- Corneal surgery and disorders
University of Bergen
2015-2025
Norwegian Cancer Society
1981-1983
Norsk Hydro (Norway)
1981-1983
BDNF signaling contributes to protein-synthesis-dependent synaptic plasticity, but the dynamics of TrkB and mechanisms translation have not been defined. Here, we show that long-term potentiation (LTP) consolidation in dentate gyrus live rodents requires sustained (hours) BDNF-TrkB signaling. Surprisingly, this activation maintains an otherwise labile pathway from MAP-kinase-interacting kinase (MNK). MNK activity promotes eIF4F initiation complex formation protein synthesis mechanistically...
Messenger RNA trafficking, which provides a mechanism for local protein synthesis, is dependent on cis ‐acting sequences in the 3′ untranslated regions (3′UTRs) of mRNAs concerned acting together with trans proteins. The C‐MYC transcription factor proto‐oncogene product involved cell proliferation, differentiation and apoptosis. Localization c ‐myc mRNA to perinuclear cytoplasm its association cytoskeleton determined by signal 3′UTR. Here we show specific binding localization element 3′UTR...
Protein-mRNA interactions affect mRNA transport, anchorage, stability and translatability in the cytoplasm. During purification of three subpopulations polysomes, it was observed that a 36-kDa protein, identified as annexin II, is associated with only one specific population namely cytoskeleton-associated polysomes. This association appears to be calcium-dependent since sensitive EGTA could reconstituted vitro. UV irradiation resulted partial, EGTA-resistant cross-linking II Binding...
Purpose.: Congenital stromal corneal dystrophy (CSCD) is an autosomal dominant condition with clouding of the cornea due to opacities. It caused by mutations in decorin gene (DCN) leading expression a truncated form decorin. In attempt replicate this mice, knock-in mouse strain, 952delT Dcn, was created. Methods.: Mice were constructed targeted mutation. Sequencing genomic DNA confirmed correct genotype. Mouse and human corneas, including corneas from patients CSCD, primary keratocyte...
Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation functions are tightly regulated by its post-translational modifications. AnxA2 Tyr23-phosphorylated form (pTyr23AnxA2) involved in malignant cell transformation, metastasis angiogenesis. Here, we show that H2O2 exerts rapid, simultaneous opposite effects on the Tyr23 phosphorylation status of two distinct compartments rat pheochromocytoma (PC12) cells. Reactive oxygen species induce...
Various post-translational modifications (PTMs) regulate the localisation and function of multifunctional protein Annexin A2 (AnxA2). In addition to its various tasks as a cytoskeletal- membrane-associated protein, AnxA2 can trans-acting binding cis-acting sequences specific mRNAs. present study, we have examined role Ser25 phosphorylation in subcellular interaction with mRNP complexes. Subcellular fractionation confocal microscopy rat neuroendocrine PC12 cells showed that...
Annexin A2 is a multifunctional protein and its cellular functions are regulated by post‐translational modifications ligand binding. When purified from porcine intestinal mucosa transformed mouse Krebs II cells, SDS–PAGE revealed high‐molecular‐mass forms in addition to the 36 kDa protomer. These were identified as poly‐/multi‐ubiquitin conjugates of annexin A2, ubiquitination represents novel modification this protein. Subcellular fractionation cells an enrichment A2‐ubiquitin Triton X‐100...
Annexin A2 (AnxA2) is a widely expressed multifunctional protein found in different cellular compartments. In spite of lacking hydrophobic signal peptide, AnxA2 at the cell surface endothelial cells, indicative role angiogenesis. Increased extracellular levels tumours correlate with neoangiogenesis, metastasis and poor prognosis. We hypothesised that may contribute to angiogenesis by affecting cell-cell interactions motility. To address this question, we studied effect heterotetrameric...
Direct acting antivirals (DAAs) provide efficient hepatitis C virus (HCV) therapy and clearance for a majority of patients, but are not available or effective all patients. They risk developing HCV-induced hepatocellular carcinoma (HCC), which the mechanism remains obscure is missing. Annexin A2 (AnxA2) has been reported to co-precipitate with non-structural (NS) HCV proteins NS5B NS3/NS4A, indicating role in HCC tumorigenesis effect on DAA therapy.Surface plasmon resonance biosensor...
For several millennia, leaves of Echium amoenum Fisch. & C. A. Mey., an important Iranian medicinal plant with nutritional value as nutraceutical, have been used tea for the treatment conditions, including inflammation. The intake E. has mainly correlated to its rich content water-soluble antioxidants. Although entire is utilized, only natural products flowers previously thoroughly investigated. rare...
Introduction: Annexin A2 (AnxA2) plays a critical role in cell transformation, immune response, and resistance to cancer therapy. Besides functioning as calcium- lipidbinding protein, AnxA2 also acts an mRNA-binding for instance, by interacting with regulatory regions of specific cytoskeleton-associated mRNAs. Methods Results: Nanomolar concentrations FL3, inhibitor the translation factor eIF4A, transiently increases expression PC12 cells stimulates shortterm transcription/translation anxA2...
The functions of the annexin family proteins involve binding to Ca2+, lipid membranes, other proteins, and RNA, annexins share a common folded core structure at C terminus. Annexin A11 (AnxA11) has long N-terminal region, which is predicted be disordered, binds forms membraneless organelles involved in neuronal transport. Mutations AnxA11 have been linked amyotrophic lateral sclerosis (ALS). We studied stability identified short stabilising segment end core, links domains I IV. crystal...
The progression of aggressive cancer occurs via angiogenesis and metastasis makes these processes important targets for the development anti-cancer agents. However, recent studies have raised concern that selective inhibition results in a switch towards increased tumour growth metastasis. Since Annexin A2 (AnxA2) is involved both metastasis, it may serve as an ideal target simultaneous processes. Based on discovery domains I (D(I)) IV (D(IV)) AnxA2 are potent inhibitors angiogenesis, we...
Localization of the actin-, lipid- and mRNA-binding protein Annexin A2 (AnxA2) in dividing cells revealed its presence large spherical structures which are confined to cell periphery frequently co-align with astral microtubules. These emerge at prometaphase disappear telophase, coinciding mitotic breakdown reformation nuclear envelope, respectively. Their size increases towards anaphase, while their number decreases, indicating that they capable fusion. Treatment propylene glycol caused...