A5047360025- Microbial Community Ecology and Physiology
- Environmental DNA in Biodiversity Studies
- Fecal contamination and water quality
- Marine and coastal ecosystems
- Identification and Quantification in Food
- Marine Biology and Ecology Research
- Genomics and Phylogenetic Studies
- Groundwater flow and contamination studies
- Atmospheric and Environmental Gas Dynamics
- Biosensors and Analytical Detection
- Water Treatment and Disinfection
- Methane Hydrates and Related Phenomena
- Environmental Justice and Health Disparities
- Protist diversity and phylogeny
- Aquatic Ecosystems and Phytoplankton Dynamics
- Mercury impact and mitigation studies
- Coral and Marine Ecosystems Studies
- Groundwater and Isotope Geochemistry
- Coastal and Marine Management
- Marine and fisheries research
- Fish Ecology and Management Studies
- Gut microbiota and health
- Marine and coastal plant biology
- Antimicrobial Resistance in Staphylococcus
- Marine Bivalve and Aquaculture Studies
National Oceanic and Atmospheric Administration
2016-2025
NOAA Atlantic Oceanographic and Meteorological Laboratories
2015-2024
NOAA National Marine Fisheries Service Southwest Fisheries Science Center
2012-2024
Office of Ocean Exploration and Research
2022-2024
NOAA National Marine Fisheries Service
2022-2023
AstraZeneca (United States)
2019
Indiana University School of Medicine
2005
Indiana University
2005
University of Miami
2001-2004
United States Geological Survey
1998
Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of marine microbial biodiversity and function world's oceans. It is simultaneous global mega-sequencing campaign aiming generate largest standardized data set in single day. This will be achievable only through coordinated efforts an Consortium, supportive partnerships networks between sites. commentary outlines establishment, aims Consortium...
Living resources in the sea are essential to economic, nutritional, recreational, and health needs of billions people. Variation biodiversity that characterizes marine systems, which underlies numerous ecosystem services provided humans, is being rapidly altered by changing environmental factors human activity. Understanding underlying causes these patterns, forecasting where future changes likely occur, requires monitoring patterns organism abundance, diversity, distribution health;...
Environmental DNA (eDNA) can be used to identify macroorganisms and describe biodiversity, thus has promise supplement biological monitoring in marine ecosystems. Despite this promise, scaling sample acquisition the size temporal scales needed for effective would require prohibitively large investments time human resources. To improve upon these problems, here we test efficacy of an autonomous eDNA sampling system compare results obtained traditional methods. The instrument consisted Sample...
This report documents the presence of fecal indicators and bacterial pathogens in sand at 53 California marine beaches using both culture-dependent -independent (PCR quantitative PCR [QPCR]) methods. Fecal indicator bacteria were widespread beach sand, with Escherichia coli enterococci detected 68% 94% surveyed, respectively. Somatic coliphages a Bacteroidales human-specific marker 43% 13% beaches, Dry samples from almost 30% contained least one following pathogens: Salmonella spp.,...
Many ocean policies mandate integrated, ecosystem-based approaches to marine monitoring, driving a global need for efficient, low-cost bioindicators of ecological quality. Most traditional methods assess biological quality rely on specialized expertise provide visual identification limited set specific taxonomic groups, time-consuming process that can narrow view status. In addition, microbial assemblages drive food webs but are not amenable inspection and thus largely excluded from detailed...
Abstract DNA metabarcoding is an important tool for molecular ecology. However, its effectiveness hinges on the quality of reference sequence databases and classification parameters employed. Here we evaluate performance MiFish 12S taxonomic assignments using a case study California Current Large Marine Ecosystem fishes to determine best practices metabarcoding. Specifically, use taxonomy cross‐validation by identity framework compare between global database comprised all available sequences...
Cyanobacterial harmful algal blooms (cyanoHABs) degrade freshwater ecosystems globally. Microcystis aeruginosa often dominates cyanoHABs and produces microcystin (MC), a class of hepatotoxins that poses threats to human animal health. Microcystin toxicity is influenced by distinct structural elements across diversity related molecules encoded variant
Abstract Environmental DNA (eDNA) data make it possible to measure and monitor biodiversity at unprecedented resolution scale. As use‐cases multiply scientific consensus grows regarding the value of eDNA analysis, public agencies have an opportunity decide how where fit into their mandates. Within United States, many federal state are individually using in various applications developing relevant expertise. A national strategy for implementation would capitalize on recent developments,...
Production rates of bromoform (CHBr 3 ), methylene bromide (CH 2 Br and methyl iodide I) were measured in the laboratory for 11 species marine macroalgae. volatile bromomethanes extrapolated to a global scale suggest that macroalgae produce × 10 g yr −1 (1 9 mol 98% which is bromoform. Laminarians (kelps) 61% this organic Br. These calculations are important biogeochemical cycling Seawater concentrations CHBr , CH I determined from various southern California coastal locales. High seawater...
The absence of standardized methods for quantifying faecal indicator bacteria (FIB) in sand hinders comparison results across studies. purpose the study was to compare extraction from sands and recommend a technique.
Abstract Environmental DNA (eDNA) is an emerging and powerful method for use in marine research, conservation, management, yet time‐ resource‐intensive protocols limit the scale of implementation. Long‐range autonomous underwater vehicles equipped with environmental sample processors (LRAUV‐ESPs) provide a new means scaling up eDNA collection processing. Here, we used metabarcoding four marker genes (mitochondrial 12S rRNA, bacterial archaeal 16S nuclear 18S mitochondrial COI), which...
The ecological and oceanographic processes that drive the response of pelagic ocean microbiomes to environmental changes remain poorly understood, particularly in coastal upwelling ecosystems. Here we show seasonal interannual variability predicts microbiome diversity community structure Southern California Current region. Ribosomal RNA gene sequencing, targeting prokaryotic eukaryotic microbes, from samples collected seasonally during 2014-2020 indicate nitracline depth is most robust...
A marine methylotroph, designated strain MB2T, was isolated for its ability to grow on methyl bromide as a sole carbon and energy source. Methyl chloride iodide also supported growth, did methionine glycine betaine. limited amount of growth observed with dimethyl sulfide. Growth noted unidentified components the complex media broth 2216, yeast extract Casamino acids. No methylated amines, methanol, formate, acetate, glucose or variety other substrates. resulted in their oxidation CO2...
Coral reefs are dynamic ecosystems known for decades to be endangered due, in large part, anthropogenic impacts from land-based sources of pollution (LBSP). In this study, we utilized an Illumina-based next-generation sequencing approach characterize prokaryotic and fungal communities samples collected off the southeast coast Florida. Water coastal inlet discharges, oceanic outfalls municipal wastewater treatment plants, treated effluent before discharge, open ocean samples, coral tissue...
Abstract Challenge 2 of the UN Ocean Decade focuses on protecting and restoring marine ecosystems biodiversity as a fundamental requirement to achieve sustainable development. Addressing this challenge requires reliable timely information ecosystems. To this, academic, government, private groups should engage in process co-design that aims facilitate decision-making at local national level, agree common interoperable practices for collection curation biology ecosystem information....