A5043655662- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Marine Toxins and Detection Methods
- Environmental DNA in Biodiversity Studies
- Marine Biology and Ecology Research
- Underwater Vehicles and Communication Systems
- Protist diversity and phylogeny
- Identification and Quantification in Food
- Morphological variations and asymmetry
- Advanced ceramic materials synthesis
- Fluid Dynamics and Vibration Analysis
- Advanced Chemical Sensor Technologies
- Fluid Dynamics and Turbulent Flows
- High-pressure geophysics and materials
- Biomimetic flight and propulsion mechanisms
- Bat Biology and Ecology Studies
- Methane Hydrates and Related Phenomena
- Water Quality Monitoring Technologies
- Microstructure and mechanical properties
- Fish Ecology and Management Studies
- Genomics and Phylogenetic Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Respiratory viral infections research
- Amphibian and Reptile Biology
- Underwater Acoustics Research
Monterey Bay Aquarium Research Institute
2013-2024
University of Victoria
2020
University of Oregon
2020
Ocean Networks Canada Society
2020
Woods Hole Oceanographic Institution
2019
Sensors (United States)
2018
Oceanography Society
2017
Albany Research Institute
2012
Lawrence Livermore National Laboratory
2007-2010
University of California, Berkeley
1999-2004
SUMMARY We compare computational, experimental and quasi-steady forces in a generic hovering wing undergoing sinusoidal motion along horizontal stroke plane. In particular, we investigate unsteady effects two-dimensional (2D)computations three-dimensional (3D) experiments several qualitatively different kinematic patterns. all cases, the computed drag compares well with experiments. The lift agrees cases which changes angle of attack are symmetrical or advanced respect to positions, but lags...
SUMMARY The elevated aerodynamic performance of insects has been attributed in part to the generation and maintenance a stable region vorticity known as leading edge vortex (LEV). One explanation for stability LEV is that spiraling axial flow within core drains energy into tip vortex, forming leading-edge spiral analogous structure generated by delta wing aircraft. However, whereas conspicuous feature flapping wings at Reynolds numbers (Re) 5000, similar experiments Re=100 failed identify...
We used two-dimensional digital particle image velocimetry (DPIV) to visualize flow patterns around the flapping wing of a dynamically scaled robot for series reciprocating strokes starting from rest. The base was equipped with strain gauges so that pattern fluid motion could be directly compared time history force production. results show development and shedding vortices throughout each stroke are highly stereotyped influence generation in subsequent strokes. When starts rest, it generates...
Divers inspecting the subsurface instrument package during pre-deployment testing.
Abstract An ecologically and economically disruptive harmful algal bloom (HAB) affected much of the northeast Pacific margin in 2015, during a prolonged oceanic warm anomaly. Caused by diatoms genus Pseudo‐nitzschia , this HAB produced highest particulate concentrations biotoxin domoic acid (DA) ever recorded Monterey Bay, California. Bloom inception followed strong upwelling spring transition, which introduced nutrients eliminated anomaly locally. Subsequently, moderate intermittent created...
Environmental DNA (eDNA) can be used to identify macroorganisms and describe biodiversity, thus has promise supplement biological monitoring in marine ecosystems. Despite this promise, scaling sample acquisition the size temporal scales needed for effective would require prohibitively large investments time human resources. To improve upon these problems, here we test efficacy of an autonomous eDNA sampling system compare results obtained traditional methods. The instrument consisted Sample...
The Environmental Sample Processor (ESP) is a device that allows for the underwater, autonomous application of DNA and protein probe array technologies as means to remotely identify quantify, in situ, marine microorganisms substances they produce. Here, we added functionality ESP through development incorporation module capable solid-phase nucleic acid extraction quantitative PCR (qPCR). Samples collected by instrument were homogenized chaotropic buffer compatible with direct detection...
Abstract An observational study was performed in the central Southern California Bight Spring 2010 to understand relationship between seasonal spring phytoplankton blooms and coastal processes that included nutrient input from upwelling, wastewater effluent plumes, other processes. Multi‐month Webb Slocum glider deployments combined with MBARI environmental sample processors (ESPs), weekly pier sampling, ocean color data provided a multidimensional characterization of development evolution...
A team of coordinated robots autonomously observed and sampled open-ocean microbial communities in a Lagrangian mode.
Metagenomic and metatranscriptomic time-series data covering a 52-day period in the fall of 2016 provide an inventory bacterial archaeal community genes, transcripts, taxonomy during intense dinoflagellate bloom Monterey Bay, CA, USA. The dataset comprises 84 metagenomes (0.8 terabases), 82 metatranscriptomes (1.1 88 16S rRNA amplicon libraries from samples collected on 41 dates. also includes 18S libraries, characterizing eukaryotic bloom. Accompanying sequence are chemical biological...
Abstract The collection of environmental DNA (eDNA) samples is often laborious, costly, and logistically difficult to accomplish at high frequency in remote locations over large geographic areas. Here, we addressed those challenges by combining two robotic technologies: an uncrewed surface vessel (USV) fitted with automated eDNA sample device survey waters the eastern North Pacific Ocean from Alameda, CA Honolulu, HI. USV Surveyor SD 1200 (Saildrone) carrying Environmental Sample Processor...
A sandwich hybridization assay (SHA) was developed to detect 16S rRNAs indicative of phylogenetically distinct groups marine bacterioplankton in a 96-well plate format as well low-density arrays printed on membrane support. The were used field-deployable instrument, the Environmental Sample Processor (ESP). SHA employs chaotropic buffer for both cell homogenization and hybridization, thus target sequences are captured directly from crude homogenates. Capture probes seven nine different...
Using autonomous molecular analytical devices embedded within an ocean observatory, we studied harmful algal bloom (HAB) ecology in the dynamic coastal waters of Monterey Bay, California. During studies 2007 and 2008, HAB species abundance toxin concentrations were quantified periodically at two locations by Environmental Sample Processor (ESP) robotic biochemistry systems. Concurrently, environmental variability processes characterized sensors co‐located with ESP network nodes, regional...
Faecal indicator bacteria (FIB) and harmful algal blooms (HABs) threaten the health economy of coastal communities worldwide. Emerging automated sampling technologies combined with molecular analytical techniques could enable rapid detection micro-organisms in-situ, thereby improving resource management public decision-making. We evaluated this concept using a robotic device, Environmental Sample Processor (ESP). The ESP automates in-situ sample collection, nucleic acid extraction analyses....
Dimethylsulfoniopropionate (DMSP) is an abundant organic sulfur metabolite produced by many phytoplankton species and degraded bacteria via two distinct pathways with climate-relevant implications. We assessed the diversity abundance of possessing these in context community composition over a 3-week time period spanning September-October, 2014 Monterey Bay, CA. The dmdA gene from DMSP demethylation pathway dominated pool was harboured mostly members alphaproteobacterial SAR11 clade...
Phytoplankton communities residing in the open ocean, largest habitat on Earth, play a key role global primary production. Through their influence nutrient supply to euphotic zone, open-ocean eddies impact magnitude of production and its spatial temporal distributions. It is important gain deeper understanding microbial ecology marine ecosystems under eddy physics with aid advanced technologies. In March April 2018, we deployed autonomous underwater surface vehicles cyclonic North Pacific...
Abstract Autonomous water sampling technologies may help to overcome the human resource challenges of monitoring biological threats rivers over long time periods and across large geographic areas. The Monterey Bay Aquarium Research Institute has pioneered a robotic Environmental Sample Processor (ESP) that overcomes some constraints associated with traditional since it can automate sample filtration preservation captured material. ESP was originally developed for marine environment...
Biomolecular analyses are used to investigate the dynamics of cyanobacterial harmful algal blooms (cyanoHABs), with samples collected during monitoring often analyzed by qPCR and sometimes amplicon metagenomic sequencing. However, cyanoHAB research programs face operational constraints due reliance on human resources for sample collections. To address this impediment, a third-generation Environmental Sample Processor (3G ESP) integrated long-range autonomous underwater vehicle (LRAUV) was...
Recent advances in ocean observing systems and genomic technologies have led to the development of deep-sea environmental sample processor (D-ESP). The D-ESP filters particulates from seawater at depths up 4000 m applies a variety molecular assays particulates, including quantitative PCR (qPCR), identify particular organisms genes situ. Preserved samples enable laboratory-based validation situ results expanded studies diversity gene expression. Tests methane-rich mound Santa Monica Basin...
Abstract The organic sulfur compound dimethylsulfoniopropionate (DMSP) is synthesized by numerous species of marine phytoplankton, and its volatile degradation products are a major source biogenic to the atmosphere. A massive bloom dinoflagellate Akashiwo sanguinea occurred in Monterey Bay, CA, USA, fall 2016 led exceptionally high seawater DMSP concentrations that peaked at 4,240 nM. Bacterial consumption rates showed only small fraction standing stock flowed through dissolved pool per day,...