A5022988974- Antimicrobial agents and applications
- Microbial Natural Products and Biosynthesis
- Dendrimers and Hyperbranched Polymers
- Bacterial biofilms and quorum sensing
- Biofuel production and bioconversion
- Genomics and Phylogenetic Studies
- Legionella and Acanthamoeba research
- Fungal Biology and Applications
- Enzyme Production and Characterization
- Plant-Microbe Interactions and Immunity
- Lignin and Wood Chemistry
- Antimicrobial Peptides and Activities
- Microbial Community Ecology and Physiology
- Antifungal resistance and susceptibility
- Studies on Chitinases and Chitosanases
- Plant Disease Resistance and Genetics
- Polydiacetylene-based materials and applications
- Microbial Metabolites in Food Biotechnology
- Biochemical and biochemical processes
- Organoboron and organosilicon chemistry
- Enzyme-mediated dye degradation
- Luminescence and Fluorescent Materials
- Plant Pathogens and Fungal Diseases
- Protist diversity and phylogeny
- Antibiotic Resistance in Bacteria
Universidad de Alcalá
2013-2023
John Innes Centre
1991-1993
Centro de Investigaciones Biológicas Margarita Salas
1989
A new laccase (EC 1.10.3.2) produced by Streptomyces cyaneus CECT 3335 in liquid media containing soya flour (20 g per liter) was purified to homogeneity. The physicochemical, catalytic, and spectral characteristics of this enzyme, as well its suitability for biobleaching eucalyptus kraft pulps, were assessed. had a molecular mass 75 kDa an isoelectric point 5.6, optimal pH temperature 4.5 70 degrees C, respectively. activity strongly enhanced the presence Cu(2+), Mn(2+), Mg(2+) completely...
Cationic carbosilane dendrimers of generations 1–3 have been synthesized employing thiol–ene click chemistry. The obtained present three different types ammonium functions, two them with the charge at surface, –NH3+ and –NMe3+, other internalized by presence ethylalcohol moieties, –[NMe2(CH2CH2OH)]+. influence –NMe3+ –[NMe2(CH2CH2OH)]+ in dendrimer structure studied molecular dynamics. antibacterial properties these families evaluated against Gram-positive (Staphylococcus aureus CECT 240)...
The bactericidal activity of a library cationic dendritic systems depends on the hydrophilic/hydrophobic structural balance, being presence sulfur atom proximal to peripheral ammonium functions relevance.
A new family of amine- and ammonium-terminated carbosilane dendrimers the type Gn-[Si(CH2)3N(Et)CH2CH2NMe2]x Gn-{[Si(CH2)3N+R(Et)CH2CH2N+RMe2]x(X−)y} (where n = 1, 2 3; R H, X Cl; Me, I) respectively has been synthesized by hydrosilylation N,N-dimethyl-N′-allyl-N′-ethyl-ethylenediamine, [(CH2CH–CH2)(Et)N(CH2)2NMe2] with corresponding hydride-terminated subsequent quaternization HCl or MeI. Quaternized are soluble stable in water other protic solvents for long time periods. The antibacterial...
Novel amine- and ammonium-terminated carbosilane dendrimers of type Gn-[Si{CH2O-(C6H4)-3-NMe2}]x or Gn-[Si{CH2O-(C6H4)-3-NMe3+I−}]x have been synthesized characterized up to second generation by phenolysis (chloromethyl)silyl-terminated with 3-dimethylamine phenol subsequent quaternization methyl iodide. Quaternized are stable in protic solvents can be solubilised water after the addition less than 1% dimethyl sulfoxide. A study antimicrobial activity these cationic first against both...
A new family of amine- and ammonium-terminated hyperbranched polycarbosilanes (PCS) dendrimers has been synthesized. The functionalization a polycarbosilane matrix was carried out with peripheral allyl groups by two strategies in the case PCS: 1) hydrosilylation amines PCS containing terminal Si–H bonds, or 2) PCS–allyl an aminosilane. Dendrimers amine were synthesized allydimethylamine. Quaternized systems MeI are soluble stable water other protic solvent. antibacterial properties have...
Thalassosaline waters produced by the concentration of seawater are widespread and common extreme aquatic habitats. Their salinity varies from that sea water (ca. 3.5%) to saturation for NaCl 37%). Obviously microbiota dramatically throughout this range. Recent metagenomic analysis intermediate (19%) indicated presence an abundant yet undescribed gamma-proteobacterium. Two strains belonging group have been isolated saltern ponds in two Spanish salterns were named "Spiribacter".The genomes...
Over the last decades, multidrug-resistant bacteria have emerged and spread, increasing number of bacteria, against which commonly used antibiotics are no longer effective. It has become a serious public health problem whose solution requires medical research in order to explore novel effective antimicrobial molecules. On one hand, peptides (AMPs) regarded as good alternatives because their generally broad-spectrum activities, but sometimes they can be easily degraded by organism or toxic...
A Gram-negative, heterotrophic, aerobic, pale orange-pigmented, non-endospore-forming and motile bacterial strain, designated strain SP8(T), was isolated from a salty water sample the solar salterns of Santa Pola, located on Mediterranean coast Spain. The grew optimally at 37 degrees C, pH 6.5 in presence 10 % NaCl. polyphasic taxonomic study conducted order to characterize detail. Phylogenetic analyses based 16S rRNA gene sequence comparisons indicated that SP8(T) clustered within branch...
Antibiotic resistance is currently one of the main threats to public health security. Biofilm formation a mechanism that responsible for most human bacterial infections and requires new effective therapeutic approaches, such as those provided by nanotechnology. In this work, antibacterial effect carbosilane metallodendrimers with different metals (copper(II) ruthenium(II)), ligands (chloride nitrate) generations (generation 0, 1 2) has been studied using planktonic Gram-positive...
Novel water-soluble ammonium-terminated carbosilane dendrons containing a ferrocene unit at the focal point were synthesized and evaluate them as new class of potential organometallic-based antibacterial compounds.
Candida spp. are one of the most common fungal pathogens. Biofilms formed by Candidaalbicans offer resistance mechanisms against antifungal agents. Therefore, development new molecules effective these microorganisms, alone or in combination with drugs, is extremely necessary. In present work, we carried out a screening process different cationic carbosilane dendritic C. albicans. vitro activity biofilm formation and biofilms was tested both Colección Española de Cultivos Tipo (CECT) 1002...
A Gram-negative, heterotrophic, aerobic, reddish-orange-pigmented, non-spore-forming, non-motile bacterial strain, designated 7-UAH(T), was isolated from salty water the athalassohaline lagoon at El Hito, located in central Spain. The strain grew optimally 37 degrees C and pH 7.0 presence of 2.5 % NaCl. polyphasic taxonomic study carried out order to characterize detail. Phylogenetic analyses based on 16S rRNA gene sequence comparisons indicated that 7-UAH(T) clustered within branch...
Two transcripts corresponding to the whiB sporulation gene of Streptomyces coelicolor A3(2) that differed in length at their 5' ends by 164 nucleotides were identified S1 mapping. Their presumptive promoters from each other; more downstream, P2, resembled typical prokaryotic (i.e., those recognized major form RNA polymerase) five six positions its -10 and -35 regions; upstream, P1, was comparably similar, instead, previously described hrdDp1 promoter (M. J. Buttner, K. F. Chater, M. Bibb,...
SUMMARY Amoebae were isolated from contact lenses of a symptomatic lens wearer in Spain. Protozoa characterized by studying their morphology, biology, protease activity and the 18S rRNA gene sequence. Morphology organism was observed light microscopy, scanning electron microscopy transmission microscopy. Its structure corresponded to an amphizoic amoeba. The protozoa grew well at 37 °C poorly lower temperatures. In addition, it capable lysing mammalian cells vitro . A major 56 kDa...
A novel pink-pigmented bacterial strain, UAH-SP71 T , was isolated from a saltern located in Santa Pola, Alicante (Spain) and the complete genome sequence analysed compared with that of Spiribacter salinus M19-40 suggesting two strains constituted separate species, 77.3 % ANI value. In this paper, strain investigated taxonomic study using polyphasic approach. Strain Gram-stain-negative, strictly aerobic, non-motile curved rod grew media containing 5–20 (w/v) NaCl (optimum 10 NaCl), at 5–40...