A5080883976- Fermentation and Sensory Analysis
- Phytochemicals and Antioxidant Activities
- Horticultural and Viticultural Research
- Gut microbiota and health
- Tea Polyphenols and Effects
- Probiotics and Fermented Foods
- Biochemical and biochemical processes
- Biochemical Analysis and Sensing Techniques
- Microbial Metabolites in Food Biotechnology
- Diet and metabolism studies
- Wine Industry and Tourism
- Meat and Animal Product Quality
- Metabolomics and Mass Spectrometry Studies
- Essential Oils and Antimicrobial Activity
- Protein Hydrolysis and Bioactive Peptides
- Oral microbiology and periodontitis research
- Nuts composition and effects
- Antioxidant Activity and Oxidative Stress
- Food composition and properties
- Nutritional Studies and Diet
- Sensory Analysis and Statistical Methods
- Biofuel production and bioconversion
- Polyamine Metabolism and Applications
- Ginseng Biological Effects and Applications
- Microbial Metabolism and Applications
Universidad Autónoma de Madrid
2015-2024
Instituto de Investigación en Ciencias de la Alimentación
2015-2024
Consejo Superior de Investigaciones Científicas
2000-2024
Hospital General Universitario Gregorio Marañón
2019
Hospital General Universitario De Valencia
2019
Universidad Complutense de Madrid
2014
National Research Council
2013
Instituto de Fermentaciones Industriales
2001-2010
Universitat de Barcelona
2010
Instituto de Química Física Blas Cabrera
2007
The ORAC-fluorescein (ORAC−FL) method recently validated using automatic liquid handling systems has now been adapted to manual and a conventional fluorescence microplate reader. As calculated for Trolox, the precision of was <3.0, expressed as percent coefficient variation. accuracy <2.3, variation mean. detection quantification limits were those corresponding 0.5- 1-μM Trolox standard solutions, respectively. applied 10 pure compounds (benzoic cinnamic acids aldehydes, flavonoids,...
We have investigated the antioxidant activity of hydrolysates from whey proteins bovine α-lactalbumin (α-La) and β-lactoglobulin A (β-Lg A) by commercial proteases (pepsin, trypsin, chymotrypsin, thermolysin, Corolase PP). PP was most appropriate enzyme to obtain α-La β-Lg (ORAC−FL values 2.315 2.151 μmol Trolox equivalent/mg protein, respectively). total 42 peptide fragments were identified HPLC-MS/MS in hydrolysate PP. One sequences (Trp-Tyr-Ser-Leu-Ala-Met-Ala-Ala-Ser-Asp-Ile) possessed...
The monomeric, oligomeric, and polymeric flavan-3-ol composition of wines, grape seeds, skins from Vitis vinifera L. cv. Graciano, Tempranillo, Cabernet Sauvignon has been studied using (1) fractionation by polyamide column chromatography followed HPLC/ESI-MS analysis, (2) on C(18) Sep-Pak cartridges reaction with vanillin acid-catalyzed degradation in the presence toluene-alpha-thiol (thiolysis). content monomers ((+)-catechin (-)-epicatechin), procyanidin dimers (B3, B1, B4, B2), trimers...
This paper reports the anthocyanin adsorption profiles of cell walls different Saccharomyces strains isolated from grapes collected in Spanish appellation controlée regions La Rioja, Navarra, and Ribera del Duero. These are habitually used red wine-making. The acyl derivatives anthocyanins (acetyl p-coumaryl compounds) were more strongly adsorbed than nonacyl derivatives. Peonidin-3G was also adsorbed, as its greater presence acetyl wall adsorbate leads to an increase yellow color a...
Tacrine and melatonin are well-known drugs with activities as an acetylcholinesterase (AChE) inhibitor free radical scavenger, respectively. In this work, we report new hybrids of both that display higher in vitro properties than the sum their parts. As selective inhibitors human AChE, IC50 values range from sub-nanomolar to picomolar. They exhibit a oxygen absorbance capacity does predicted be able cross blood−brain barrier reach targets central nervous system.
Hydroxycinnamic acid content and ferulic dehydrodimer were determined in 11 barley varieties after alkaline hydrolysis. Ferulic (FA) was the most abundant hydroxycinnamate with concentrations ranging from 359 to 624 μg/g dry weight. p-Coumaric (PCA) levels ranged 79 260 weight, caffeic present at of <19 Among dehydrodimers that identified, 8-O-4'-diFA (73−118 weight), followed by 5,5'-diFA (26−47 8,5'-diFA benzofuran form (22−45 open (10−23 weight). Significant variations (p < 0.05) among...
With the aim of investigating potential flavan-3-ols to influence growth intestinal bacterial groups, we have carried out in vitro fermentation, with human faecal microbiota, two purified fractions from grape seed extract (GSE): GSE-M (70% monomers and 28% procyanidins) GSE-O (21% 78% procyanidins). Samples were collected at 0, 5, 10, 24, 30 48 h fermentation for enumeration by fluorescent situ hybridization analysis phenolic metabolites. Both promoted Lactobacillus/Enterococcus decrease...
An in vitro batch culture fermentation experiment was conducted with fecal inocula from three healthy volunteers the presence and absence of a red wine extract. Changes main bacterial groups were determined by FISH during 48 h period. The catabolism flavonoids (i.e., flavan-3-ols anthocyanins) formation wide range phenolic microbial metabolites targeted UPLC-PAD-ESI-TQ MS method. Statistical analysis revealed that catechol/pyrocatechol, as well 4-hydroxy-5-(phenyl)-valeric, 3-...
Avocado processing by the food and cosmetic industries yields a considerable amount of phenolic-rich byproduct such as peels seeds. Utilization these byproducts would be favorable from an economic point view. Methanolic (80%) extracts obtained lyophilized ground seeds avocado (Persea americana Mill.) Hass Shepard varieties were characterized for their phenolic compound profiles using HPLC-PAD technique. The structures identified compounds subsequently unambiguously confirmed ESI-MS....
An exhaustive study of the phenolic composition almond (Prunus dulcis (Mill.) D.A. Webb) skins was carried out in order to evaluate their potential application as a functional food ingredient. Using HPLC-DAD/ESI-MS technique, total 33 compounds corresponding flavanols, flavonols, dihydroflavonols and flavanones, other nonflavonoid were identified. Peaks another 23 structure-related also detected. MALDI-TOF MS applied characterize skin proanthocyanidins, revealing existence series A- B-type...
ABSTRACT: Almond ( Prunus dulcis [Mill.] D.A. Webb) skins have been proposed as a source of bioactive polyphenols. In this article, the phenolic composition and antioxidant activity almond obtained from different processes (blanching [freeze‐drying], blanching + drying, roasting) were studied. A total 31 compounds corresponding to flavan‐3‐ols (33% 56% identified), flavonol glycosides (9% 36%), hydroxybenzoic acids aldehydes (6% 26%), aglycones (1.7% 18%), flavanone (3% 7.7%), (0.69% 5.4%),...