- Insect and Pesticide Research
- Pesticide Residue Analysis and Safety
- Analytical Chemistry and Chromatography
- Bee Products Chemical Analysis
- Plant and animal studies
- Chromatography in Natural Products
- Microfluidic and Capillary Electrophoresis Applications
- Analytical chemistry methods development
- Fermentation and Sensory Analysis
- Advanced Chemical Sensor Technologies
- Phytochemicals and Antioxidant Activities
- Genomics, phytochemicals, and oxidative stress
- Meat and Animal Product Quality
- Insect and Arachnid Ecology and Behavior
- Analytical Methods in Pharmaceuticals
- Pesticide and Herbicide Environmental Studies
- Pharmacological Effects and Assays
- Essential Oils and Antimicrobial Activity
- Toxic Organic Pollutants Impact
- Glaucoma and retinal disorders
- Pesticide Exposure and Toxicity
- Retinal Diseases and Treatments
- Mass Spectrometry Techniques and Applications
- Ocular Infections and Treatments
- Cancer Treatment and Pharmacology
Universidad de Valladolid
2014-2023
Ministerio de Defensa
2006
Ministry of Health
2006
Instituto de Salud Carlos III
2006
Queen Victoria Hospital
2006
Hospital Universitario Virgen del Rocío
1997
Summary In recent years, honeybees ( Apis mellifera ) have been strangely disappearing from their hives, and strong colonies suddenly become weak died. The precise aetiology underlying the disappearance of bees remains a mystery. However, during same period, Nosema ceranae , microsporidium Asian bee cerana seems to colonized A. it's now frequently detected all over world in both healthy honeybee colonies. For first time, we show that natural N. infection can cause sudden collapse colonies,...
Honeybee colony collapse is a sanitary and ecological worldwide problem. The features of this syndrome are an unexplained disappearance adult bees, lack brood attention, reduced strength, heavy winter mortality without any previous evident pathological disturbances. To date there has not been consensus about its origins. This report describes the clinical two professional bee-keepers affecting by syndrome. Anamnesis, examination analyses support that depopulation in both cases was due to...
In the last decade, an increase in honey bee (Apis mellifera L.) colony losses has been reported several countries. The causes of this decline are still not clear. This study was set out to evaluate pesticide residues stored pollen from colonies and their possible impact on Spain. total, 1,021 professional apiaries were randomly selected. All samples subjected multiresidue analysis by gas chromatography-mass spectrometry (MS) liquid chromatography-MS; moreover, specific methods applied for...
In recent years, a worldwide decline in the Apis mellifera populations has been detected many regions, including Spain. This is thought to be related effects of pathogens or pesticides, although what extent these factors are implicated still not clear. this study, we estimated prevalence honey bee colony depopulation symptoms random selected sample (n = 61) and explored implication different pathogens, pesticides flora visited area under study. The professional apiaries studied was 67.2%...
Here we present a holistic screening of collapsing colonies from three professional apiaries in Spain. Colonies with typical honey bee depopulation symptoms were selected for multiple possible factors to reveal the causes collapse. Omnipresent Nosema ceranae and Lake Sinai Virus. Moderate prevalences found Black Queen Cell Virus trypanosomatids, whereas Deformed Wing Virus, Aphid Lethal Paralysis strain Brookings neogregarines rarely detected. Other viruses, apis, Acarapis woodi Varroa...
The present study examines the use of Kahoot! as a gamification tool to explore mixed learning strategies. We analyze its in two different groups theoretical subject third course Degree Chemistry. An empirical-analytical methodology was used using students, with frequencies. academic results these group students were compared between them and those obtained previous course, which not employed, aim measuring evolution students´ knowledge. showed, all cases, that has led significant increase...
A study of the real possibilities carbohydrate profiles and chemometrics to characterize botanical origin honey from a single geographical area, Province Soria (Spain), is presented. To this end, 14 carbohydrates were quantified using high-performance liquid chromatography (HPLC) with pulsed amperometric detection (PAD) in 77 natural honeys, origins which ling, spike lavender, French thyme, forest, multifloral. Principal component analysis has been employed as first approach samples...
PURPOSE: To assess the presence of mitomycin-C (MMC) in hen aqueous humor after photorefractive keratectomy (PRK). SETTING: Instituto Universitario de Oftalmobiología Aplicada, Faculty Medicine, University Valladolid, and Department Analytical Chemistry, Sciences, Spain. METHODS: Mitomycin-C 0.02% was applied topically for 2 minutes to a right hen's eye PRK (Group A) left with intact epithelium B). At different time points (10, 30, 60, 360, 720 minutes), extracted high-performance liquid...