- Insect and Pesticide Research
- Plant and animal studies
- Bee Products Chemical Analysis
- Insect and Arachnid Ecology and Behavior
- Essential Oils and Antimicrobial Activity
- Allergic Rhinitis and Sensitization
- Automotive and Human Injury Biomechanics
- Traffic and Road Safety
- Advanced Chemical Sensor Technologies
- Fermentation and Sensory Analysis
- Injury Epidemiology and Prevention
- Spectroscopy and Chemometric Analyses
- Insect Pest Control Strategies
- Insect-Plant Interactions and Control
- Plant Reproductive Biology
- Phytochemicals and Antioxidant Activities
- Lichen and fungal ecology
- Healthcare and Venom Research
- Horticultural and Viticultural Research
Centro Regional de Selección y Reproducción Animal
2020-2023
Regional Government of Castile-La Mancha
2011-2022
Consejería de Agricultura, Pesca, Agua y Desarrollo Rural
2010
Summary In recent years, honeybees ( Apis mellifera ) have been strangely disappearing from their hives, and strong colonies suddenly become weak died. The precise aetiology underlying the disappearance of bees remains a mystery. However, during same period, Nosema ceranae , microsporidium Asian bee cerana seems to colonized A. it's now frequently detected all over world in both healthy honeybee colonies. For first time, we show that natural N. infection can cause sudden collapse colonies,...
Honeybee colony collapse is a sanitary and ecological worldwide problem. The features of this syndrome are an unexplained disappearance adult bees, lack brood attention, reduced strength, heavy winter mortality without any previous evident pathological disturbances. To date there has not been consensus about its origins. This report describes the clinical two professional bee-keepers affecting by syndrome. Anamnesis, examination analyses support that depopulation in both cases was due to...
In the last decade, an increase in honey bee (Apis mellifera L.) colony losses has been reported several countries. The causes of this decline are still not clear. This study was set out to evaluate pesticide residues stored pollen from colonies and their possible impact on Spain. total, 1,021 professional apiaries were randomly selected. All samples subjected multiresidue analysis by gas chromatography-mass spectrometry (MS) liquid chromatography-MS; moreover, specific methods applied for...
In recent years, a worldwide decline in the Apis mellifera populations has been detected many regions, including Spain. This is thought to be related effects of pathogens or pesticides, although what extent these factors are implicated still not clear. this study, we estimated prevalence honey bee colony depopulation symptoms random selected sample (n = 61) and explored implication different pathogens, pesticides flora visited area under study. The professional apiaries studied was 67.2%...
Here we present a holistic screening of collapsing colonies from three professional apiaries in Spain. Colonies with typical honey bee depopulation symptoms were selected for multiple possible factors to reveal the causes collapse. Omnipresent Nosema ceranae and Lake Sinai Virus. Moderate prevalences found Black Queen Cell Virus trypanosomatids, whereas Deformed Wing Virus, Aphid Lethal Paralysis strain Brookings neogregarines rarely detected. Other viruses, apis, Acarapis woodi Varroa...
In this work, 7 Spanish honeys with different botanical origins were studied. The honey rosemary, chestnut, lavender, echium, thyme, multifloral, and honeydew. chemical compounds determined ascorbic acid (vitamin C), hydroxymethylfurfural, major sugar contents (glucose fructose). physicochemical parameters, pH, conductivity, moisture, free acidity, color, also measured. Vitamin C is an important antioxidant in food, the possibility to use it as discriminate parameter among was determination...
Different monofloral honeys from Castilla-La Mancha (Spain) have been studied in order to determine their main functional and biological properties. Thyme honey chestnut possess the highest antioxidant capacity, which is due high vitamin C (in thyme honey) total polyphenolic content honey). On other hand, showed antimicrobial activity against Staphylococcus aureus Escherichia coli, whilst others had no S. very small E. coli. Moreover it was found that measured partly its lysozyme content. In...
SummaryIn this report, the pathological features observed in honey bees naturally infected with microsporidium Nosema ceranae throughout a year are described. The lesions produced under natural conditions similar to those experimentally caged worker bees. N. is able reproduce inside epithelial cells and multiply all through causing host-cells, characterized by progressive irreversible destruction of layer ventriculus, which can lead major negative repercussions on normal process digestion,...
Honey consumption and imports have increased in recent years, it is considered by consumers to be a healthy alternative more commonly used sweeteners. contains mixture of polyphenols antioxidant compounds, the botanical origin geographical area collection play an important role on its chemical composition. The present study investigated physicochemical properties, total phenolic content capacity Spanish thyme honey chestnut honey, their mixtures with royal jelly (2% 10%) propolis 10%)....
A diverse supply of pollen is an important factor for honey bee health, but information about the diversity available to colonies at landscape scale largely missing. In this COLOSS study, beekeeper citizen scientists sampled and analyzed collected by colonies. As a simple measure diversity, beekeepers determined number colors found in samples that were coordinated standardized way. Altogether, 750 from 28 different regions 24 countries participated two-year study almost 18,000 samples....
Bee pollen is currently one of the most commonly consumed food supplements, as it considered to be a good source bioactive substances and energy. It contains various health-promoting compounds, such proteins, amino acids, lipids, well glucosinolates. In present study, glucosinolate content was determined, by means ultra-performance liquid chromatography coupled quadrupole time-of-flight mass detector, in 72 bee samples from four different apiaries Guadalajara (Spain), harvested three...
Bee pollen is currently one of the most widely consumed dietary supplements due to its high nutritional value and potentially beneficial effects on health. Unfortunately, in recent years an increase fraudulent marketing this product has been detected, mainly terms adulteration with from other sources. This made it necessary seek new tools ensure authentication. Therefore, study investigates use free amino acids as markers geographical origin harvesting period bee pollen. To demonstrate their...
In the present study, mineral composition of seventy-one bee pollen samples from four different apiaries was determined by means inductively coupled plasma-optical emission spectrometry. The results showed that there were no significant differences in relation to overall content per sample terms apiary origin or harvesting period; most common elements phosphorus and potassium with concentrations ranging 2.3 5.1 g/kg (dry weight). Moreover, analyzed similar higher than recommended as...
A cross-sectional study was carried out in Galicia, NW Spain, order to estimate the magnitude of honey bee colony losses and identify potential risk factors involved. total 99 samples from apiaries were collected spring using simple random sampling. According international guidelines, classified as affected by loss or asymptomatic. Each sample consisted worker bees, brood comb-stored pollen. All bees analysed individually detect main pathogens. Moreover, presence residues most prevalent...
In the last years, an increase has been observed in adulteration of bee pollen. Consequently, different tools are required to authenticate origin this product, such as a study profile and composition specific family compounds. The present investigates potential betaines related compounds markers apiary harvest period 71 pollen samples. These were collected from four apiaries (Pistacho, Tío Natalio, Monte Fuentelahiguera), located same geographical area (Guadalajara, Spain) sampled during...
In this case report, we analyze the possible causes of poor health status a professional Apis mellifera iberiensis apiary located in Gajanejos (Guadalajara, Spain). Several factors that potentially favor colony collapse were identified, including Nosema ceranae infection, alone or combination with other (e.g., BQCV and DWV infection), accumulation acaricides commonly used to control Varroa destructor beebread (coumaphos tau-fluvalinate). Based on levels residues, average toxic unit estimated...
The objective of this work was to determine the influence soil substrate on characteristics and properties a specific type honey. As such, we analysed features typical single-flower honey, thyme produced in Mediterranean region. Thymus is genus aromatic perennial plants that are native Europe, North Africa Asia. A total 70 honey samples from hives situated limestone (38 samples) or gypsum soils (32 were studied. physical chemical each analyzed using standard assays. Within same geographical...
Honey samples (n = 126) from Castilla-La Mancha (Central Spain) were characterized based on their physicochemical properties and a melissopalynological analysis. The latter showed that Echium pollen type was the dominant palynomorph in most samples, representing at least 30% of each sample. As anticipated, relationship observed between proportion this honey. One goal study to set threshold defines percentage necessary for Viper's bugloss honey be considered monofloral or multifloral. This is...
To study the influence of thiamethoxam exposure on colony strength and pathogen prevalence, an apiary (5 colonies) was placed in front a plot sown with winter oilseed rape (wOSR), just before flowering phase. Before sowing, seeds were treated equivalent application 18 g thiamethoxam/ha. For comparison, second located separate 750 m untreated wOSR. Dead foragers at entrance hives assessed every 2-3 days throughout period, while (number combs covered adult honey bees brood) pathogens monitored...