A5046838036- Microbial Metabolites in Food Biotechnology
- Studies on Chitinases and Chitosanases
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Nanocomposite Films for Food Packaging
- Biofuel production and bioconversion
- Diet, Metabolism, and Disease
- Microbial Metabolic Engineering and Bioproduction
- Biomedical and Engineering Education
- Carbohydrate Chemistry and Synthesis
- Protein Hydrolysis and Bioactive Peptides
- Enzyme-mediated dye degradation
- Neurotransmitter Receptor Influence on Behavior
- Metal-Catalyzed Oxygenation Mechanisms
- Plant biochemistry and biosynthesis
- Cannabis and Cannabinoid Research
- Microbial Metabolism and Applications
- Cholinesterase and Neurodegenerative Diseases
- Evolution and Genetic Dynamics
- Protein purification and stability
- Aquaculture Nutrition and Growth
- Problem and Project Based Learning
- Glycosylation and Glycoproteins Research
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
Universidad Europea
2018-2024
Instituto de Catálisis y Petroleoquímica
2015-2022
University of Vienna
2019
Instituto de Química Orgánica General
2019
University of Saint Francis Xavier
2019
Universidad Complutense de Madrid
2019
Consejo Superior de Investigaciones Científicas
2014-2015
Chitinases are ubiquitous enzymes that have gained a recent biotechnological attention due to their ability transform biological waste from chitin into valued chito-oligomers with wide agricultural, industrial or medical applications. The activity of these molecules is related size and acetylation degree. Chitinase Chit42 Trichoderma harzianum hydrolyses oligomers minimal three N-acetyl-D-glucosamine (GlcNAc) units. Gene chit42 was previously characterized, according its sequence, the...
Among several commercial enzymes screened for chitosanolytic activity, Neutrase 0.8L (a protease from Bacillus amyloliquefaciens) was selected in order to obtain a product enriched deacetylated chitooligosaccharides (COS). The hydrolysis of different chitosans with this enzyme followed by size exclusion chromatography (SEC-ELSD), mass spectrometry (ESI-Q-TOF), and high-performance anion-exchange pulsed amperometric detection (HPAEC-PAD). converted 10 g/L various into mostly oligosaccharides,...
Chitooligosaccharides (COS) are homo- or hetero-oligomers of D-glucosamine (GlcN) and N-acetyl-D-glucosamine (GlcNAc) that can be obtained by chitosan chitin hydrolysis. Their enzymatic production is preferred over other methodologies (physical, chemical, etc.) due to the mild conditions required, fewer amounts waste its efficiency control product composition. By properly selecting enzyme (chitinase, chitosanase nonspecific enzymes) substrate properties (degree deacetylation, molecular...
The β-galactosidase from Bacillus circulans was covalently attached to aldehyde-activated (glyoxal) agarose beads and assayed for the continuous production of galactooligosaccharides (GOS) in a packed-bed reactor (PBR). immobilization fast (1 h) activity resulting biocatalyst 97.4 U/g measured with o-nitrophenyl-β-d-galactopyranoside (ONPG). showed excellent operational stability 14 successive 20 min reaction cycles at 45 °C batch reactor. A process GOS synthesis operated 213 h 0.2 mL/min...
By controlled hydrolysis of chitosan or chitin with different enzymes, three types chitooligosaccharides (COS) MW between 0.2 and 1.2 kDa were obtained: fully deacetylated (fdCOS), partially acetylated (paCOS), (faCOS). The chemical composition the samples was analyzed by high-performance anion exchange chromatography pulsed amperometric detection (HPAEC-PAD) MALDI-TOF mass spectrometry. synthesized fdCOS basically formed GlcN, (GlcN)2, (GlcN)3, (GlcN)4. On contrary, faCOS contained mostly...
Vinyl sulfone-silica was efficient to covalently immobilize levansucrase at neutral pH. LEV-VS showed altered selectivity towards FOS and good operational stability.
The biological activity of chitooligosaccharides (COS) has made them targets for industrial and medical sectors. In this work, endo-chitinase Chit33 from Trichoderma harzianum CECT 2413 was expressed in Pichia pastoris GS115 to levels never achieved before (630 mg/L; 3.3 U/mL), without its biochemical characteristics being substantially affected. produced a mixture fully partially acetylated COS different chitin derivatives. HPAEC-PAD Chromatography mass spectrometry analyses showed that...
Rubisco is an ancient, catalytically conserved yet slow enzyme, which plays a central role in the biosphere's carbon cycle. The design of Rubiscos to increase agricultural productivity has hitherto relied on use vivo selection systems, precluding exploration biochemical traits that are not wired cell survival. We present directed -in vitro- evolution platform extracts enzyme from its biological context provide new avenue for engineering. Precambrian and extant form II were subjected ensemble...
Unspecific peroxygenases (UPOs) are highly promiscuous biocatalyst with self-sufficient mono(per)oxygenase activity. A laboratory-evolved UPO secreted by yeast was covalently immobilized in activated carriers through one-point attachment. In order to maintain the desired orientation without compromising enzyme's activity, S221C mutation introduced at surface of enzyme, enabling a single disulfide bridge be established between support and protein. Fluorescence confocal microscopy demonstrated...
Fungi infection, especially derived from Plasmopara viticola, causes severe grapevine economic losses worldwide. Despite the availability of chemical treatments, looking for eco-friendly ways to control Vitis vinifera infection is gaining much more attention. When a plant infected, multiple disease-control molecular mechanisms are activated. PRRs (Pattern Recognition Receptors) and particularly RLKs (receptor-like kinases) take part in first barrier immune system, and, as consequence, kinase...
Abstract Acidic xylooligosaccharides (XOS), also called aldouronics, are hetero-oligomers of xylose randomly branched with 4-O-methyl-D-glucuronic acid residues linked by α(1 → 2) bonds, which display bioactive properties. We have developed a simple and integrated method for the production acidic XOS enzymatic hydrolysis glucurono-xylan from beechwood. Among enzymes screened, Depol 670L (a cellulolytic preparation Trichoderma reesei ) displayed highest activity (70.3 U/mL, expressed in...
INTEGRATING SUBJECTS THROUGH PROJECT-BASED LEARNING METHODOLOGIES: A NOVEL ACADEMIC MODEL IN THE DEGREE OF BIOTECHNOLOGY AT UNIVERSIDAD EUROPEA