A5013741181- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Biosensors and Analytical Detection
- Streptococcal Infections and Treatments
- Clostridium difficile and Clostridium perfringens research
- Genomics and Phylogenetic Studies
- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Antibiotics Pharmacokinetics and Efficacy
- Yersinia bacterium, plague, ectoparasites research
- Antimicrobial Resistance in Staphylococcus
- Urinary Tract Infections Management
- Actinomycetales infections and treatment
- Oral microbiology and periodontitis research
- Phytoplasmas and Hemiptera pathogens
- Biochemical and Molecular Research
- Mobile Health and mHealth Applications
- Gut microbiota and health
- Enterobacteriaceae and Cronobacter Research
- Mycotoxins in Agriculture and Food
- 3D Printing in Biomedical Research
- Dental Research and COVID-19
- Spectroscopy Techniques in Biomedical and Chemical Research
Software (Spain)
2020-2024
Clover Biopharmaceuticals (China)
2024
Universidad de Granada
2019-2024
Parque Tecnológico de la Salud
2020
Mycobacterium abscessus is one of the most common and pathogenic nontuberculous mycobacteria (NTM) isolated in clinical laboratories. It consists three subspecies: M. subsp. , bolletii massiliense .
The Enterobacter cloacae complex (ECC) encompasses heterogeneous clusters of species that have been associated with nosocomial outbreaks. These may different acquired antimicrobial resistance and virulence mechanisms, their identification is challenging. This study aims to develop predictive models based on matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) profiles machine learning for species-level identification. A total 219 ECC 118 Klebsiella...
MALDI-TOF MS is considered to be an important tool for the future development of rapid microbiological techniques. We propose application as a dual technique identification bacteria and detection resistance, with no extra hands-on procedures.
Salivary pH serves as a valuable and useful diagnostic marker for periodontal disease, it not only plays critical role in disease prevention but also its development. Typically, saliva sampling is collected by draining spitting into collection tubes or using swabs. In this study, we have developed Point-of-Care (POC) device situ determination of oral without the need complex instruments, relying solely on smartphone detection device. Our system utilizes non-toxic vegetable colourimetric...
Vancomycin-resistant Enterococcus faecium represents a health threat due to its ability spread and cause outbreaks. MALDI-TOF MS has demonstrated usefulness for E. identification, but implementation antimicrobial resistance detection is still under evaluation. This study assesses the repeatability of peak analysis performance in discrimination vancomycin-susceptible (VSE) from vancomycin-resistant isolates (VRE). The was carried out on protein spectra 178 unique clinical isolates-92 VSE, 31...
Hypervirulent ribotypes (HVRTs) of Clostridioides difficile such as ribotype (RT) 027 are epidemiologically important. This study evaluated whether MALDI-TOF can distinguish between strains HVRTs and non-HVRTs commonly found in Europe. Obtained spectra clinical C. isolates (training set, 157 isolates) covering relevant Europe were used an input for different machine learning (ML) models. Another 83 a validation set. Direct comparison obtained from did not allow to discriminate these two...
A microfluidic paper-based analytical device integrating a chromoreactand – formylazo dye– has been fabricated and used for colorimetric assay of sulfites. The was covalently linked to paper by vinyl sulfone chemistry. This work presents two robust capillary devices determine sulfite in wine without any pretreatment. One them based on thread (µTPAD) useful it white another (µPAD) specifically red as well wine. Both are the selective recognition means that turns from orange yellow presence...
In this study, we evaluate the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid detection carbapenemase activity in Enterobacterales clinical microbiology laboratories during a multicenter networking validation study. The study was divided into three different stages: “software design,” “intercenter evaluation,” and “clinical validation.” First, standardized procedure with an online software data analysis designed....
Clostridioides difficile is a leading cause of hospital-acquired diarrhea, presenting significant clinical challenges due to its high morbidity and mortality rates role in nosocomial outbreaks. Rapid accurate detection toxigenic ribotypes crucial for effective outbreak control. We developed diagnostic methodology utilizing MALDI-TOF MS Machine Learning algorithms differentiate C. ribotypes. Analysis spectra from 379 isolates 10 Spanish hospitals identified seven biomarker peaks...
Matrix-assisted laser desorption-ionization/time of flight mass spectrometry (MALDI-TOF MS) has been widely implemented for the rapid identification microorganisms. Although most bacteria, yeasts and filamentous fungi can be accurately identified with this method, some closely related species still represent a challenge MALDI-TOF MS. In study, two MALDI-TOF-based approaches were applied discrimination at species-level isolates belonging to Cryptococcus neoformans complex, previously...
The increasing emergence of carbapenemase-producing Klebsiella pneumoniae (CPK) isolates is a global health alarm. Rapid methods that require minimum sample preparation and rapid data analysis are urgently required. Matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) has recently been used by clinical laboratories for identification antibiotic-resistant bacteria; however, discrepancies have arisen regarding biological technical issues. aim this study was...
Abstract Clostridioides difficile is the main cause of antibiotic related diarrhea and some ribotypes (RT), such as RT027, RT181 or RT078, are considered high risk clones. A fast reliable approach for C. ribotyping needed a correct clinical approach. This study analyses high-molecular-weight proteins with MALDI-TOF MS. Sixty-nine isolates representative most common in Europe were analyzed 17,000-65,000 m/z region classified into 4 categories (RT027, RT181, RT078 ‘Other RTs’). Five supervised...
The excessive use of piperacillin/tazobactam (P/T) has promoted the emergence P/T-resistant Enterobacterales. We reported that in Escherichia coli, P/T contributes to development extended-spectrum resistance β-lactam/β-lactamase inhibitor (BL/BLI) (ESRI) isolates are susceptible but have low-level BL/BLI. Currently, detection relying on conventional methods is time-consuming. To overcome this issue, we developed a cost-effective test based MALDI-MS technology, called MALDIpiptaz, which aims...
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently been used for the direct detection KPC-producing isolates by analysis 11,109 Da peak representing P019 protein. In this study, we evaluate presence in a collection 435 unduplicated Klebsiella pneumoniae clinical isolates. The prevalence blaKPC K. was 49.2% (32/65). not observed any other carbapenemase (319) or noncarbapenemase producers (116). Computational p019 gene performed...
Early use of infection control methods is critical for preventing the spread antimicrobial resistance. Whole-genome sequencing (WGS) considered gold standard investigating outbreaks; however, turnaround time usually too long clinical decision-making and method also costly. The aim this study was to evaluate performance Fourier transform infrared (FTIR) artificial intelligence tools as a first-line typing tool carbapenemase-producing Klebsiella pneumoniae (CPK) in hospital setting. For...
MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of Flight) is a type of mass spectrometry (MS) that has been widely implemented for the rapid identification microorganisms over last decade. The accuracy and flexibility this method encouraged researchers to implement analysis protein spectra obtained by discrimination close-related species bacterial typing. In study, standardized methodology based on detection species-specific peaks from with described. was applied collection...
The goal of this study was to evaluate matrix-assisted laser desorption ionization–iime flight mass spectrometry (MALDI-TOF MS) and Fourier-transform infrared spectroscopy (FTIR-S) as diagnostic alternatives DNA-based methods for the detection Pseudomonas aeruginosa sequence type (ST) 175 isolates involved in a hospital outbreak. For purpose, 27 P. from an outbreak detected Hematology department our were analyzed by above-mentioned methodologies. Previously, these had been characterized...
Objectives : Evaluation of Matrix-Assisted Laser Desorption Ionization-Time Flight Mass Spectrometry (MALDI-TOF MS) and Fourier Transform Infrared-Spectroscopy (FTIR-S) as diagnostic alternatives to DNA-based methods for the detection Pseudomonas aeruginosa sequence type (ST) 175 isolates involved in a hospital outbreak. Methods Twenty-seven P. from 2014 outbreak Hematology department our were previously characterized by PFGE WGS. Besides, 8 analyzed unrelated controls. MALDI-TOF MS spectra...
ABSTRACT Vancomycin-resistant Enterococcus faecium has become a health threat over the last 20 years due to its ability rapidly spread and cause outbreaks in hospital settings. Although MALDI-TOF MS already demonstrated usefulness for accurate identification of E. , implementation antimicrobial resistance detection is still under evaluation. The reproducibility peak analysis performance correct discrimination vancomycin susceptible isolates (VSE) from those hosting VanA VanB mechanisms was...