A5045208507- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Enterobacteriaceae and Cronobacter Research
- Antibiotics Pharmacokinetics and Efficacy
- Urinary Tract Infections Management
- Mycobacterium research and diagnosis
- Infective Endocarditis Diagnosis and Management
- Streptococcal Infections and Treatments
- Yersinia bacterium, plague, ectoparasites research
- Biosensors and Analytical Detection
- Antimicrobial Resistance in Staphylococcus
- Actinomycetales infections and treatment
- Clostridium difficile and Clostridium perfringens research
- Nosocomial Infections in ICU
- Bacterial Genetics and Biotechnology
- Antifungal resistance and susceptibility
- Infections and bacterial resistance
- Antibiotic Use and Resistance
- Gut microbiota and health
- Pharmaceutical and Antibiotic Environmental Impacts
- Ginseng Biological Effects and Applications
- Plant Pathogens and Fungal Diseases
- Tuberculosis Research and Epidemiology
- Pneumonia and Respiratory Infections
- Mass Spectrometry Techniques and Applications
Instituto de Investigación Biomédica de A Coruña
2018-2025
Complexo Hospitalario Universitario A Coruña
2016-2025
Instituto de Salud Carlos III
2022-2025
Centro de Investigación Biomédica en Red
2021-2024
Red Espanola de Investigacion en Patologia Infecciosa
2022
Universidade da Coruña
2019
Abstract Objectives To characterize the antimicrobial susceptibility, molecular epidemiology and carbapenem resistance mechanisms in Pseudomonas aeruginosa isolates recovered from respiratory tract samples patients with ventilator-associated pneumonia enrolled MagicBullet clinical trial. Methods Isolates were collected 53 12 hospitals Spain, Italy Greece. Susceptibility was determined using broth microdilution Etest. MALDI-TOF MS used to detect carbapenemase activity carbapenemases...
Development of a novel MALDI-TOF MS-based method for the rapid detection ESBL-producing Enterobacteriaceae.The was evaluated in terms sensitivity, specificity and turnaround time regarding antibiotic used (cefotaxime, ceftazidime, ceftriaxone, cefpodoxime or cefepime) performance automated MBT STAR-BL software (Bruker Daltonik GmbH, Germany) relative to qualitative interpretation spectra detecting β-lactamase resistance by MS Daltonik) collection 11 isogenic Escherichia coli control strains...
The global distribution of carbapenemases such as KPC, OXA-48, and metallo-β-lactamases (MBLs) gives cause for concern, these enzymes are not inhibited by classical β-lactamase inhibitors (BLIs). current development new is one the most promising highlights treatment multidrug-resistant bacteria. activity cefepime in combination with novel BLIs zidebactam, taniborbactam, enmetazobactam was studied a collection 400 carbapenemase-producing Enterobacterales (CPE). genomes were fully sequenced...
Infections caused by ceftolozane-tazobactam and ceftazidime-avibactam-resistant P. aeruginosa infections are an emerging concern. We aimed to analyze the underlying ceftazidime-avibactam resistance mechanisms in all multidrug-resistant or extensively drug-resistant (MDR/XDR) isolates recovered during 1 year (2020) from patients with a documented infection. Fifteen showing were evaluated. Clinical conditions, previous positive cultures, β-lactams received month reviewed for each patient. MICs...
The ability of MALDI-TOF for the identification nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on reproducibility have been performed so far, none mycobacteria. aim this study was evaluate NTM in 15 laboratories 9 European countries. A total 98 clinical isolates were grown Löwenstein-Jensen. Biomass collected tubes with water ethanol, anonymized sent out participating laboratories....
Imipenem/relebactam is a novel carbapenem/β-lactamase inhibitor combination, developed to act against carbapenemase-producing Enterobacterales (CPE).To assess the in vitro activity of imipenem/relebactam Spanish nationwide collection CPE by testing susceptibility these isolates 16 widely used antimicrobials and determine underlying β-lactam resistance mechanisms involved molecular epidemiology carbapenemases Spain.Clinical (n = 401) collected for 2 months from 24 hospitals Spain were tested....
ABSTRACT Pseudomonas aeruginosa is a major nosocomial pathogen commonly involved in multidrug-resistant (MDR) infections that are very difficult to treat. Imipenem/relebactam new carbapenem/β-lactamase inhibitor combination with robust activity against P. . However, resistance increasingly reported, and rapid detection is, therefore, crucial so appropriate treatments can be prescribed. We have developed MALDI TOF-MS-based method accurately predict the presence of carbapenemases using...
Objectives: Development of an automated MALDI-TOF MS-based method for the rapid, direct detection carbapenemase-producing Enterobacteriaceae in clinical urine samples within 90 min sample reception.
MALDI-TOF MS is considered to be an important tool for the future development of rapid microbiological techniques. We propose application as a dual technique identification bacteria and detection resistance, with no extra hands-on procedures.
In this study, we evaluate the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid detection carbapenemase activity in Enterobacterales clinical microbiology laboratories during a multicenter networking validation study. The study was divided into three different stages: “software design,” “intercenter evaluation,” and “clinical validation.” First, standardized procedure with an online software data analysis designed....
A rapid and sensitive (100%) matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) assay was developed to detect OXA-48-type producers, using 161 previously characterized clinical isolates. Ertapenem monitored carbapenem resistance, temocillin included in the as a marker for OXA-48-producers. Structural analysis is described. Data are obtained within 60 min.
ObjectivesEvaluation of the LightMix® modular carbapenemase kits for rapid detection carbapenemase-producing Enterobacteriaceae (CPE) and application these to direct colonized patients bacteraemias.
Objectives: Rapid detection of the plasmid-mediated quinolone resistance determinant AAC(6′)-Ib-cr in Enterobacteriaceae by measuring acetyltransferase activity against fluoroquinolones MALDI-TOF MS analysis. Methods: The presence enzyme was determined a collection 81 isogenic Escherichia coli control strains [10 carrying during exposure to ciprofloxacin, norfloxacin and levofloxacin] further analysis 36 clinical isolates [25 addition different combinations mechanisms]. effect acetylation...
The increasing emergence of carbapenemase-producing Klebsiella pneumoniae (CPK) isolates is a global health alarm. Rapid methods that require minimum sample preparation and rapid data analysis are urgently required. Matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) has recently been used by clinical laboratories for identification antibiotic-resistant bacteria; however, discrepancies have arisen regarding biological technical issues. aim this study was...