The Pectobacteriumcarotovorum species corresponds to a complex, including two subspecies with validly published names, proposed and new species, Pectobacterium polaris aquaticum. Recent studies suggested that this complex needed revision. We examined the taxonomic status of 144 strains isolated from wide range plant various geographical origins waterways. Sequences leuS, dnaX recA housekeeping genes clustered 114 these together within not yet described clade. sequenced eight clade analysed...

Abstract Planktothrix is a dominant cyanobacterial genus forming toxic blooms in temperate freshwater ecosystems. We sequenced the genome of planktic and non strains to better represent this diversity life style at genomic level. Benthic biphasic are rooting phylogenetic tree widely expand pangenome genus. further investigated silico genetic potential dedicated gas vesicles production, nitrogen fixation as well natural product synthesis conducted complementary experimental tests by cell...

This work aimed to establish the taxonomic status of six strains (A212-S19-A16T, A127-S21-F16, A105-S21-F16, A104-S21-F16, A101-S19-F16 and A35-S23-M15) isolated from three different waterways in 2015 2016 south-east France. Amplification sequencing gapA housekeeping gene clustered these together inside genus Pectobacterium outside already described or proposed species supspecies. Phenotypic analysis, using GENIII Biolog plates performed with A212-S19-A16T, closely related polaris (CFBP...

Strains 2B12T, FVG1-MFV-O17 and FVG10-MFV-A16 were isolated from fresh water samples collected in Asia Europe. The nucleotide sequences of the gapA barcodes revealed that all three strains belonged to same cluster within genus Dickeya. Using 13 housekeeping genes (fusA, rpoD, rpoS, glyA, purA, groEL, gapA, rplB, leuS, recA, gyrB, infB secY), multilocus sequence analysis confirmed existence a new clade. When genome these isolates other Dickeya species compared, silico DNA-DNA hybridization...

Soft rot Pectobacteriacea (SRP) gathers more than 30 bacterial species that collectively a wide range of plants by producing and secreting large set plant cell wall degrading enzymes (PCWDEs). Worldwide potato field surveys identified 15 different SRP on symptomatic tubers. The abundance each observed during outbreaks varies over space time the mechanisms driving shift outbreak are unknown. Furthermore, multi-species infections frequently dynamics these coinfections not well understood.

Dickeya solani is an emerging pathogen that causes soft rot and blackleg diseases in several crops including Solanum tuberosum, but little known about its genomic diversity evolution. We combined Illumina PacBio technologies to complete the genome sequence of D. strain 3337 was used as a reference compare with 19 other genomes (including type IPO2222T) which were generated by technology. This population analysis highlighted unexpected variability among isolates since it led characterization...

Bacterial collections are invaluable tools for microbiologists. However, their practical use is compromised by imprecise taxonomical assignation of bacterial strains. This particularly true soft rotting plant pathogens the Pectobacterium genus. We analysed taxonomic status 265 strains deposited at CIRM-CFBP collection from 1944 to 2020. gathered isolated in 27 countries 32 species representing 17 botanical families or nonhost environments. The MLSA approach completed genomic analysis 15 was...

Through this study, we established the taxonomic status of seven strains belonging to genus Pectobacterium (A477-S1-J17T, A398-S21-F17, A535-S3-A17, A411-S4-F17, A113-S21-F16, FL63-S17 and FL60-S17) collected from four different river streams an artificial lake in south-east France between 2016 2017. Ecological surveys rivers lakes pointed out repartition clade compared closest species, aquaticum. The main phenotypic difference observed these P. aquaticum type strain was strongly impaired...

Little is known about the role of antibiotics and associated resistance in microbial ecosystems absence clinical antibiotic pressure. The Soft Rot Pectobacteriaceae (SRP) species complex, which comprises 37 bacterial that are collectively responsible for severe rotting many crops, an interesting model to analyse β-lactam β-lactamases natural ecosystems. In particular, within this most Pectobacterium versatile strains harbour a β-lactamase called BlaPEC-1. aim our work was BlaPEC-1 during...

The pectinolytic enterobacteria of the Pectobacterium and Dickeya genera are causative agents maceration-associated diseases affecting a wide variety crops ornamentals. For past decade, emergence novel species D. solani was observed in potato fields Europe Mediterranean basin. purpose this study is to search by comparative genomics genetic traits that could be distinctive other involved adaptation plant host. 3337 exhibits 4.9 Mb circular genome characterized low content mobile elements with...

The genus Dickeya includes plant pathogenic bacteria attacking a wide range of crops and ornamentals as well few environmental isolates from water. Defined on the basis six species in 2005, this now 12 recognized species. Despite description several new recent years, diversity is not yet fully explored. Many strains have been analyzed for causing diseases economically important crops, such potato pathogens D. dianthicola solani . In contrast, only characterized origin or isolated plants...

ABSTRACT Bacteria carry phage‐derived elements within their genomes, some of which can produce phage‐like particles (tailocins) used as weapons to kill kin strains in response environmental conditions. This study investigates the production and activity tailocins by plant‐pathogenic bacteria: Pectobacterium , Dickeya, Musicola genera, compete for niche, providing an attractive model ecological role tailocins. Microscopy revealed that most analysed (88%) produced Tailocin‐mediated killing...

Transcriptome analysis of bacterial pathogens is a powerful approach to identify and study the expression patterns genes during host infection. However, early stages virulence at genome scale lacking with respect understanding plant-pathogen interactions diseases, especially foliar This mainly due both low ratio cells plant material beginning infection, high contamination by chloroplastic material. Here we describe reliable straightforward method for cell purification from infected leaf...

PecS is one of the major global regulators controlling virulence Dickeya dadantii, a broad-host-range phytopathogenic bacterium causing soft rot on several plant families. To define regulon during colonization, we analysed transcriptome profiles in wild-type and pecS mutant strains early colonization leaf surfaces tissue just before onset symptoms, found that consists more than 600 genes. About one-half these genes are down-regulated mutant; therefore, has both positive negative regulatory...

Summary Successful infection of a pathogen relies on the coordinated expression numerous virulence factor‐encoding genes. In plant–bacteria interactions, this control is very often achieved through integration several regulatory circuits controlling cell–cell communication or sensing environmental conditions. Dickeya dadantii (formerly Erwinia chrysanthemi ), causal agent soft rot many crops and ornamentals, provokes maceration infected plants mainly by producing secreting battery plant cell...

Members of the genus Dickeya are plant pathogens that mostly cause soft‐rot diseases. Diversity within has led to recent description three novel species (i.e. a mainly clonal D . solani economic consequence in potatoes, D. aquatica from water samples and fangzhongdai isolated necrotic pear trees China). However, multilocus sequence analysis ( MLSA ) highlighted occurrence seven isolates not related defined species. These include previously unclassified group strains monocotyledonous plants,...

The Dickeya genus is part of the Pectobacteriaceae family that included in newly described enterobacterales order. It comprises a group aggressive soft rot pathogens with wide geographic distribution and host range. Among them, new fangzhongdai species groups causative agents maceration-associated diseases impact variety crops ornamentals. affects mainly monocot plants, but D. strains have also been isolated from pear trees water sources. Here, we analysed which genetic novelty exists this...

Summary Blackleg and soft rot are devastating diseases on potato stem tuber caused by Pectobacterium Dickeya pectinolytic enterobacteria. In European cultures, D. dianthicola solani species successively emerged in the past decades. Ecological traits associated to their settlement remain elusive, especially case of recent invader . this work, we combined genomic, metabolic transcriptomic comparisons unravel common distinctive genetic functional characteristics between two isolates. The...

Specific interactions have been highlighted between cyanobacteria and chemotrophic bacteria within the cyanosphere, suggesting that nutrients recycling could be optimized by cyanobacteria/bacteria exchanges. In order to determine respective metabolic roles of cyanobacterial bacterial consortia (microbiome), a day-night metatranscriptomic analysis was performed on Dolichospermum sp. (N2 -fixer) Microcystis (non N2 natural blooms occurring successively French peri-urban lake. The taxonomical...

Although irrigation water is frequently assessed for the presence of plant pathogens, large spatial and temporal surveys that provide clues on diversity circulation pathogens are missing. We evaluate soft rot Pectobacteriaceae (SRP) genera Dickeya Pectobacterium over 2 years in a temperate, mixed-use watershed. The abundance isolated strains correlates with agricultural gradient along watershed positive correlation found temperature, nitrate, dissolved organic carbon concentration....

To compare environmental and culture-derived microbial communities, we performed 16S metabarcoding of uncultured samples their bacterial lawns. Microbial communities were obtained from freshwater river representative an anthropization gradient along a stream. Their lawns by growing aliquots the on broad range medium two different semi-selective media. The V3–V4 rRNA region was amplified sequenced. diversity water decreased upper to lower stream sampling sites and, as expected, these...

Abstract Historically, research on Soft Rot Pectobacteriacea (SRP) has focused economically important crops and ornamentals knowledge of these bacteria outside the plant context remains poorly investigated. Recently, two closely related species Pectobacterium aquaticum quasiaquaticum were isolated from water have not been any yet. To identify distinctive characteristics species, we performed a comparative genomic analysis 80 genomes representing 19 an evolutionary reconstruction. Both...

A family of affinity probes has been generated to detect and purify abscisic-acid (ABA)-binding proteins, by coupling ABA onto carrier proteins (ovalbumin or BSA) through the C1 carboxyl group C4' carbonyl ABA. ELISA detection showed that these ABA-protein conjugates bound efficiently solubilized microsomal protein fraction Arabidopsis thaliana, but not soluble fraction. Heat proteolytic treatments inhibited binding conjugates, indicating nature sites. After membrane purification microsomes,...