Plants must rapidly adapt to changes in nutrient conditions. Especially adaptations changing nitrogen environments are very complex involving also major adjustments on the protein level. Here, we used a size-exclusion chromatography-coupled mass spectrometry approach study dynamics of protein-protein interactions induced by transition from full nutrition starvation. Comparison interaction networks established for each condition revealed large overlap proteins which were part network, but...

Background Hypermethylation of CpG islands in the promoter regions tumor suppressor genes is one mechanism tumorigenesis. Caveolin-1 (Cav-1), a gene coding for structural component cellular caveolae, involved cell signaling and has been proposed to be several malignancies. This maps 7q31.1, site known deleted some prostate tumors. We chose examine methylation status region Cav-1 determine whether this could function as cancer Methods Genomic DNA from both normal epithelial cells...

Hypermethylation of CpG islands in the promoter regions tumor suppressor genes is one mechanism tumorigenesis. Caveolin-1 (Cav-1), a gene coding for structural component cellular caveolae, involved cell signaling and has been proposed to be several malignancies. This maps 7q31.1, site known deleted some prostate tumors. We chose examine methylation status region Cav-1 determine whether this could function as cancerGenomic DNA from both normal epithelial cells was obtained paraffin-embedded...

One of the most common chromosomal abnormalities in prostate cancer involves loss 10q22-qter. Rarely, a smaller deletion, involving 10q24-q25, has been observed, suggesting presence tumor suppressor gene at this site. We previously demonstrated that MXI1 maps to 10q24-q25 and is mutated some tumors with cytogenetically detectable deletions locus. encodes basic-helix-loop-helix protein suppresses transcriptional activity MYC oncoprotein by competing for dimerization partner, MAX, binding...

Protein-protein interaction studies provide valuable insights into cellular signaling. Brassinosteroid (BR) signaling is initiated by the hormone-binding receptor Insensitive 1 (BRI1) and its co-receptor BRI1 Associated Kinase (BAK1). BAK1 were shown to interact independently with Receptor-Like Protein 44 (RLP44), which implicated in BRI1/BAK1-dependent cell wall integrity perception. To demonstrate proposed complex formation of BRI1, RLP44, we established three-fluorophore intensity-based...

Brassinosteroids (BR) are key hormonal regulators of plant development. However, whereas the individual components BR perception and signaling well characterized experimentally, question how they can act whether sufficient to carry out critical function cellular elongation remains open. Here, we combined computational modeling with quantitative cell physiology understand dynamics plasma membrane (PM)-localized response pathway during initiation responses in epidermis

Super-resolution microscopy (SRM) approaches revolutionize cell biology by providing insights into the nanoscale organization and dynamics of macromolecular assemblies single molecules in living cells. A major hurdle limiting SRM democratization is post-acquisition data analysis which often complex time-consuming. Here, we present OneFlowTraX, a user-friendly open-source software dedicated to single-molecule localization (SMLM) such as single-particle tracking photoactivated (sptPALM)....

Transurethral resection of prostate (TURP) for clinically benign prostatic enlargement is a common surgical procedure which often provides voluminous material microscopic pathology examination. In about 15% cases, adenocarcinoma will be discovered incidentally in TURP tissue. Many these incidental carcinomas are stage A! and require no further clinical intervention, but significant number them A2. The question this study was to ascertain whether all or only portion tissue from routine...

Plant receptors constitute a large protein family that regulates various aspects of development and responses to external cues. Functional characterization this the identification their ligands remain major challenges in plant biology. Previously, we identified plasma membrane-intrinsic sucrose-induced receptor kinase 1 (SIRK1) Qian Shou (QSK1) as receptor/co-receptor pair involved regulation aquaporins response osmotic conditions induced by sucrose. In study, member elicitor peptide (PEP)...

Rapid processing of histopathologic material is becoming increasingly desirable to fulfill the needs clinicians treating acutely ill patients. Traditional techniques for rapid paraffin-embedded tissues require 4 5 hours, delaying treatment some critically patients and requiring additional shifts technologists in laboratory. Microwave further shortens this time, allowing even more diagnosis. Few data exist comparing quality microwave-processed tissue with that processed by traditional...

No AccessJournal of UrologyInvestigative Urology1 Feb 1996Evidence for a Tumor Suppressor Gene Distal to BRCA1 in Prostate Cancer Briana J. Williams, Emma Jones, Xiao Lin Zhu, Michael R. Steele, Robert A. Stephenson, L. Ralph Rohr, and Arthur Brothman WilliamsBriana Williams More articles by this author , JonesEmma Jones ZhuXiao Zhu SteeleMichael Steele StephensonRobert Stephenson RohrL. Rohr BrothmanArthur View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)66509-1AboutFull...

Same-day turnaround of pathology specimens is desirable in this era managed care, and rapid microwave tissue processing produces histologic features a quality equivalent to overnight processing. We studied whether microwave-assisted adversely affects the immunohistochemical staining. selected 30 (20 neoplastic 10 nonneoplastic) from our routine surgical workload. Paired large blocks were made each specimen type, one for conventional Two microarrays 60 punches donor blocks. The microarray...

The quality of gynecologic cytology has been questioned in the last two years. This author's hospital laboratory a sizable outpatient and biopsy practice from which data have obtained several different assurance projects. In this article author analyzes those with respect to detection false negative screening errors, specimen sampling precision interpretation, productivity methods. Sampling errors obtaining specimens are major problem be addressed by assurance. most sensitive efficient...

Abstract Twenty prostate tumor specimens, obtained from radical prostatectomies, and two lymph node metastases were examined by classical molecular cytogenetic methods. A sample each was analyzed histologically used for touch preparations. Adjacent samples preparation of single‐cell suspensions before cell culture (DirFISH) establishing cultures, which subsequently harvested G‐banding analysis. Fluorescence in situ hybridization (FISH) performed on preparations, DirFISH, cells tissue...

Abstract Using a polymerase chain reaction/microsatellite marker system, we demonstrated that 6 of 22 (27%) clinical stage B (early) primary prostate tumors showed loss heterozygosity at one or more five loci on chromosome 17. The sensitivity this study was increased by use Phosphorlmager and statistical analysis replicate tumor‐normal DNA pairs. Two patients tumor‐specific interstitial locus in close proximity to the familial breast cancer gene BRCAI. These findings suggest genes proximal...

The bipolar mitotic spindle is a highly conserved structure among eukaryotes that mediates chromosome alignment and segregation. Spindle assembly size control are facilitated by force-generating microtubule-dependent motor proteins known as kinesins. In animals, kinesin-12 cooperates with kinesin-5 to produce outward-directed forces necessary for assembly. plants, the relevant molecular mechanisms formation poorly defined. While an Arabidopsis thaliana ortholog has been identified, in plants...

Journal Article Spurious Automated Leukopenia Due to In Vitro Granulocyte Aggregation Get access L. Ralph Rohr, M.D, M.D Department of Pathology, Tucson Medical Center, Tucson, Arizona Address reprint requests Dr. Rohr: P.O. Box 42195. 85733. Search for other works by this author on: Oxford Academic Google Scholar Florence M. Rivers, MT(ASCP)SH American Clinical Volume 93, Issue 4, 1 April 1990, Pages 572–574, https://doi.org/10.1093/ajcp/93.4.572 Published: 01 1990 history Received: 12 June...