A5052028844- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Smart Agriculture and AI
- Polysaccharides and Plant Cell Walls
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Gene expression and cancer classification
- Scientific Computing and Data Management
- Agriculture, Soil, Plant Science
- Cell Image Analysis Techniques
- Plant Gene Expression Analysis
- Carcinogens and Genotoxicity Assessment
- DNA Repair Mechanisms
- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Research Data Management Practices
- DNA and Nucleic Acid Chemistry
- Remote Sensing in Agriculture
- Bioinformatics and Genomic Networks
- Plant Pathogenic Bacteria Studies
University of Tübingen
2018-2024
Universitätsmedizin Göttingen
2010
University of Göttingen
2010
University of Hildesheim
2010
Significance Cell-fate determination and cellular behavior in plants rely mainly on positional information intercellular communication. A plethora of cues are perceived by surface receptors integrated into an adequate output. Here, we show that the small receptor-like protein RLP44 acts as intermediary to connect for two well-known signaling molecules, brassinosteroid phytosulfokine, control cell fate root vasculature. Furthermore, receptor has functions independent from responses its...
As technical developments in omics and biomedical imaging increase the throughput of data generation life sciences, need for information systems capable managing heterogeneous digital assets is increasing. In particular, supporting findability, accessibility, interoperability, reusability (FAIR) principles scientific management.We propose a Service Oriented Architecture approach integrated management analysis multi-omics data. Our architecture introduces an image system into FAIR-supporting,...
Protein-protein interaction studies provide valuable insights into cellular signaling. Brassinosteroid (BR) signaling is initiated by the hormone-binding receptor Insensitive 1 (BRI1) and its co-receptor BRI1 Associated Kinase (BAK1). BAK1 were shown to interact independently with Receptor-Like Protein 44 (RLP44), which implicated in BRI1/BAK1-dependent cell wall integrity perception. To demonstrate proposed complex formation of BRI1, RLP44, we established three-fluorophore intensity-based...
Brassinosteroids (BR) are key hormonal regulators of plant development. However, whereas the individual components BR perception and signaling well characterized experimentally, question how they can act whether sufficient to carry out critical function cellular elongation remains open. Here, we combined computational modeling with quantitative cell physiology understand dynamics plasma membrane (PM)-localized response pathway during initiation responses in epidermis
Abstract Spatial specificity of cell fate decisions is central for organismal development. The phloem tissue mediates long-distance transport energy metabolites along plant bodies and characterized by an exceptional degree cellular specialization. How a phloem-specific developmental program implemented is, however, unknown. Here we reveal that the ubiquitously expressed PHD-finger protein OBE3 forms module with SMXL5 establishing in Arabidopsis thaliana . By interaction studies ATAC-seq...
Plants depend on various cell surface receptors to integrate extracellular signals with developmental programs. One of the best-studied is BRASSINOSTEROID INSENSITIVE 1 (BRI1) in Arabidopsis (Arabidopsis thaliana). Upon binding its hormone ligands, BRI1 forms a complex shape-complementary coreceptor and initiates signal transduction cascade, which leads variety responses. At macroscopic level, brassinosteroid (BR) biosynthetic receptor mutants have similar growth defects, initially led...
Abstract Brassinosteroids (BR) are involved in the control of several developmental processes ranging from root elongation to senescence and adaptation environmental cues. Thus, BR perception signaling have be precisely regulated. One regulator is BRI1‐associated kinase 1 (BAK1)‐interacting receptor‐like 3 (BIR3). In absence BR, BIR3 forms complexes with insensitive (BRI1) BAK1. However, biophysical energetic requirements for complex formation ligand yet determined. Using computational...
bZIP transcription factors regulate diverse processes in eukaryotic cells. Arabidopsis members of the C and S1 groups form heterodimers synergistically control metabolic reprogramming during stress responses. However, their functional characterization is complicated due to an overlapping heterodimerization network high redundancy. In this study, we develop a simple but powerful approach for generating dominant negative mutants with specificity. By applying vitro DNA-binding, reporter gene...
Abstract Protein-protein interaction studies provide valuable insights into cellular signaling. The well-studied brassinosteroid (BR) signaling is initiated by the hormone-binding receptor Brassinosteroid Insensitive 1 (BRI1) and its co-receptor BRI1 Associated Kinase (BAK1). BAK1 were shown to interact independently with Receptor-Like Protein 44 (RLP44), which implicated in BRI1/BAK1-dependent cell wall integrity perception. To demonstrate proposed complex formation of BRI1, RLP44, we...
Abstract Hormonal mechanisms associated with cell elongation play a vital role in the development and growth of plants. Here, we report Nextflow-root (nf-root), novel best-practice pipeline for deep-learning-based analysis fluorescence microscopy images plant root tissue from A. thaliana. This bioinformatics performs automatic identification developmental zones images. also includes apoplastic pH measurements, which is useful modeling hormone signaling physiological responses. We show that...
Abstract Spatial specificity of cell fate decisions is central for organismal development. The phloem tissue mediates long-distance transport energy metabolites along plant bodies and characterized by an exceptional degree cellular specialization. How a phloem-specific developmental program implemented is, however, unknown. Here we reveal that the ubiquitously expressed PHD-finger protein OBE3 forms module with phloem- specific SMXL5 establishing identity in Arabidopsis thaliana . By...
Abstract As technical developments in omics and biomedical imaging drive the increase quality, modality, throughput of data generation life sciences, need for information systems capable integrative, long-term storage management these heterogeneous digital assets is also increasing. Here, we propose an approach based on principles Service Oriented Architecture design, to allow integrated analysis multi-omics data. The proposed architecture introduces interoperable image system, OMERO server,...
Abstract Brassinosteroids (BR) are key hormonal regulators of plant development. However, whereas the individual components BR perception and signaling well characterized experimentally, question how they can act whether sufficient to carry out critical function cellular elongation remains open. Here, we combined computational modeling with quantitative cell physiology understand dynamics plasma membrane (PM)-localized response pathway during initiation responses in epidermis Arabidopsis...
Abstract Here we report nextflow-root ( nf-root ), a novel best-practice pipeline for deep learning-based analysis of fluorescence microscopy images plant root tissue, aimed at studying hormonal mechanisms associated with cell elongation, given the vital role that hormones play in development and growth plants. This bioinformatics performs automatic identification developmental zones tissue images, apoplastic pH measurements zones, which is useful modeling hormone signaling physiological...