A5038687992- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Proteoglycans and glycosaminoglycans research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Monoclonal and Polyclonal Antibodies Research
- Infant Nutrition and Health
- Cancer-related Molecular Pathways
- Probiotics and Fermented Foods
- Protease and Inhibitor Mechanisms
- Gut microbiota and health
- Neonatal Respiratory Health Research
- Lung Cancer Research Studies
- Cystic Fibrosis Research Advances
- Lung Cancer Treatments and Mutations
- Peptidase Inhibition and Analysis
- Ocular Surface and Contact Lens
- Inhalation and Respiratory Drug Delivery
- Occupational and environmental lung diseases
- Neuroendocrine Tumor Research Advances
- Antifungal resistance and susceptibility
- RNA modifications and cancer
- Advanced Drug Delivery Systems
- Reproductive tract infections research
- Microbial Metabolites in Food Biotechnology
Inserm
2011-2025
Centre Hospitalier Universitaire de Lille
2008-2025
Université de Lille
2010-2025
Institute for Translational Research in Inflammation
2020-2022
Lille Inflammation Research International Center
1998-2022
Université Lille Nord de France
2011
Centre de Recherche Jean Pierre Aubert
2008
Institut de Biologie de Lille
2005
Hôpital Claude Huriez
2000
Institut pour l'avancée des biosciences
1998
We have previously reported that 1-benzyl-2-acetamido-2-deoxy-α-d-galactopyranoside (GalNAcα-O-bn), an inhibitor of glycosylation, perturbed apical biosynthetic trafficking in polarized HT-29 cells suggesting involvement a lectin-based mechanism. Here, we identified galectin-4 as one the major components detergent-resistant membranes (DRMs) isolated from 5M12 cells. Galectin-4 was also found post-Golgi carrier vesicles. The functional role studied by using retrovirus-mediated RNA...
Some interstitial lung diseases involve pulmonary fibrosis, which is a process that characterized by the excessive and abnormal accumulation of extracellular matrix in interalveolar space. Although current anti-fibrotic therapy aims at slowing down progression it does not reverse it, many drugs were identified basic-research studies failed clinical phases, mainly because lack model can recapitulate pathophysiological mechanisms human fibrosis. We developed novel experimental fibrosis induced...
Abstract BACKGROUND Matrix metalloproteinases (MMPs) and tissue inhibitors of (TIMPs) play a role in the processes extracellular matrix degradation. Changes their expression levels have been observed various tumor types, including lung carcinoma. However, clinical significance prognostic importance progression nonsmall cell carcinoma (NSCLC) remain to be specified. In this study, mRNA MMP‐1, MMP‐9, TIMP‐1, TIMP‐2 were evaluated patients with resected NSCLC, associations disease prognosis...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
A current challenge is to define the biological characteristics of colon tumor cells resistant chemotherapy. Distinct sub-populations mucus-secreting were previously obtained from cancer cell line HT-29 after long-term treatment with anti-cancer drugs, 5-fluorouracil (5-FU) and methotrexate (MTX). Since mucins are increasingly implicated as playing a role in carcinogenesis, we studied pattern mucin expression two clones enterocyte-like phenotype which differ their capacity resist 5-FU and/or...
A weakening of the gut mucous barrier permits an increase in access intestinal luminal contents to epithelial cells, which will trigger inflammatory response. In bowel diseases, there is inappropriate and ongoing activation immune system, possibly because mucus less protective against endogenous microflora. General strategies aimed at improving protection epithelium are still missing. We generated a transgenic mouse that secreted molecule consisting 12 consecutive copies mucin domain into...
Candida albicans is part of the human gastrointestinal (GI) microbiota. To better understand how C. efficiently establishes GI colonisation, we competitively challenged growth 572 signature-tagged strains (~10% genome coverage), each conditionally overexpressing a single gene, in murine gut. We identified CRZ2, transcription factor whose overexpression and deletion respectively increased decreased early colonisation. Using clues from genome-wide expression gene-set enrichment analyses, found...
Abstract p 53 mutations and myc gene amplification expression were studied in 119 lung carcinomas of all histological types. A mutant p53 immunophenotype was previously found 47% these tumors by immunohistochemical analysis. Seven cases exhibited genomic rearrangements on Southern blots. Elevated levels transcript 12 (10%) decreased 27 (23%) Northern In most the cases, low associated with negative immunostaining, whereas elevated mRNA related to positive immunostaining (mutant...
Abstract RB protein expression and loss of heterozygosity (LOH) in the gene were studied 77 primary lung carcinomas all histological types. was by immunohistochemistry with 3 anti‐RB antibodies, found altered 23/29 (79%) neuro‐endocrine (NE) 18/48 (37%) non‐NE carcinomas. allele status probes detecting RFLP locus. Fifty‐five patients informative, detected 29 (52%) corresponding tumors 1 used; 89% informative NE carcinomas, excluding careinoids, only 13% exhibited LOH RB‐protein expression....
The mucin MUC5B has a critical protective function in the normal lung, salivary glands, esophagus, and gallbladder, been reported to be aberrantly expressed breast cancer, second leading cause of cancer-related deaths among women worldwide. To understand better implication cancer pathogenesis, luminal human cell line MCF7 was transfected with vector encoding recombinant mini-mucin then infected virus deliver short hairpin RNA knock down mini-mucin. proliferative invasive properties Matrigel...
The mucus that coats the gastrointestinal tract of all mammals is a dynamic and sticky gel layer represents first protective barrier between host hostile environment. There is, however, lack detailed knowledge about organization because high water content complexity MUC2, main gel-forming molecule in intestine. Histological staining multilabel immunofluorescence method were used to examine blankets Muc2 mouse colon ileum samples fixed Carnoy's solution, unveiling an extraordinarily complex...
Endothelial cells (ECs) are highly plastic, capable of differentiating into various cell types. Endothelial-to-mesenchymal transition (EndMT) is crucial during embryonic development and contributes substantially to vascular dysfunction in many cardiovascular diseases (CVDs). While targeting EndMT holds therapeutic promise, understanding its mechanisms modulating pathways remain challenging. Using single-cell RNA sequencing on three vitro models, we identified conserved gene signatures. We...
Abstract Gel-forming mucins are macromolecules produced by goblet cells and responsible for the mucus gel formation. Changes in cell density gel-forming mucin production have emerged as sensitive indicators mucosal diseases. A Muc5b-GFP tagged reporter mouse was used to assess Muc5b tissues immunofluorescence microscopy fluorescent activity using stereromicroscopy probe-based confocal laser endomicroscopy. followed longitudinally recording vagina embryonic lung explants under stimulation...
Abstract Mucus is the first biological barrier encountered by particles and pathogenic bacteria at surface of secretory epithelia. The viscoelasticity mucus governed in part low energy interactions that are difficult to assess. CYS domain a good candidate support between GFMs and/or constituents. Our aim was stiffen from HT29-MTX cell cocultures colon mice through delivery recombinant protein made hydrophobic domains found multiple copies polymeric mucins. ability poly-CYS molecule gels...