A5046003393- Neural dynamics and brain function
- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Neuroscience and Neuropharmacology Research
- Genetics and Neurodevelopmental Disorders
- Trypanosoma species research and implications
- Photoreceptor and optogenetics research
- Marine animal studies overview
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Calcium signaling and nucleotide metabolism
- Neuroendocrine regulation and behavior
- Neuroscience of respiration and sleep
- Retinal Development and Disorders
- Child Development and Digital Technology
- Autism Spectrum Disorder Research
- Cell Adhesion Molecules Research
- Lysosomal Storage Disorders Research
- Cellular Mechanics and Interactions
- Melanoma and MAPK Pathways
- Developmental Biology and Gene Regulation
- Research on Leishmaniasis Studies
- Connexins and lens biology
- Genomics and Chromatin Dynamics
- Neurobiology and Insect Physiology Research
Aarhus University
2022-2023
The University of Queensland
2016-2022
Institute of Pathology and Genetics
2020
Université Libre de Bruxelles
2005-2017
Enhancer function, from sponges to humans Identifying the function of enhancers, DNA regions that help regulate gene expression and evolve rapidly, has been difficult. This area research hampered by difficultly in identifying functional conservation. Wong et al. now show despite low sequence conservation, enhancer is strongly conserved through animal kingdom (see Perspective Harmston). Transgenic sponge enhancers zebrafish mice demonstrates these sequences can drive cell type–specific across...
Tricky Tryps African trypanosomes, responsible for human sleeping sickness, are known their powerful strategies of immune evasion, in particular antigenic variation. Adding another facet to this adaptive potential, Salmon et al. (p. 463 , published online 14 June; see the cover) now show that early after infection, these parasites subvert first line innate host defense by inhibiting tumor necrosis factor-α synthesis myeloid cells. This occurs through stress-induced and release cyclic...
Abstract Background Loss or disrupted expression of the FMR1 gene causes fragile X syndrome (FXS), most common monogenetic form autism in humans. Although disruptions sensory processing are core traits FXS and autism, neural underpinnings these phenotypes poorly understood. Using calcium imaging to record from entire brain at cellular resolution, we investigated neuronal responses visual auditory stimuli larval zebrafish, using fmr1 mutants model FXS. The purpose this study was alterations...
Abstract Humans resist infection by the African parasite Trypanosoma brucei owing to trypanolytic activity of serum apolipoprotein L1 (APOL1). Following uptake endocytosis in parasite, APOL1 forms pores endolysosomal membranes and triggers lysosome swelling. Here we show that induces both lysosomal mitochondrial membrane permeabilization (LMP MMP). Trypanolysis coincides with MMP consecutive release Tb EndoG endonuclease nucleus. is associated kinesin KIFC1, which motor vesicular trafficking...
Abstract Habituation is a form of learning during which animals stop responding to repetitive stimuli, and deficits in habituation are characteristic several psychiatric disorders. Due technical challenges, the brain-wide networks mediating poorly understood. Here we report calcium imaging larval zebrafish repeated visual looming stimuli. We show that different functional categories loom-sensitive neurons located locations throughout brain, both properties their resulting behavior can be...
Errors in clinical decision-making are disturbingly common. Here, we show that structured information–sharing networks among clinicians significantly reduce diagnostic errors, and improve treatment recommendations, as compared to groups of ...Errors Recent studies have found 10 15% all decisions regarding diagnoses inaccurate. experimentally study the ability ...
The imaging of neuronal activity using calcium indicators has become a staple modern neuroscience. However, without ground truths, there is real risk missing significant portion the responses. Here, we show that common assumption, non-negativity responses as detected by indicators, biases all levels frequently used analytical methods for these data. From extraction meaningful fluorescence changes to spike inference and analysis inferred spikes, each step risks because assumption...
Many features of auditory processing are conserved among vertebrates, but the degree to which these pathways established at early stages is not well explored. In this study, we have observed single cell activity throughout brains larval zebrafish with goal identifying cellular responses, brain regions, and brain-wide through larvae perceive process stimuli. Using GCaMP selective plane illumination microscopy, find strong responses tones ranging from 100 Hz 400 Hz. We also identify different...
Light-sheet microscopy is used extensively in developmental biology and neuroscience. One limitation of this approach that absorption scattering produces shadows the illuminating light sheet, resulting stripe artifacts. Here, we introduce diffuse light-sheet microscopes use a line diffuser to randomize propagation within image plane, allowing sheets reform after obstacles. We incorporate two existing configurations: selective plane illumination which sample illuminated with static sheet...
Information about water flow, detected by lateral line organs, is critical to the behavior and survival of fish amphibians. While certain aspects flow processing have been revealed through electrophysiology, we lack a comprehensive description neurons that respond network they form. Here, use brain-wide calcium imaging in combination with microfluidic stimulation map out, at cellular resolution, neuronal responses involved perceiving information larval zebrafish. We find diverse array...
The optic tectum of larval zebrafish is an important model for understanding visual processing in vertebrates. has been traditionally viewed as dominantly visual, with a majority studies focusing on the processes by which tectal circuits receive and process retinally-derived information. Recently, handful have shown much more complex role zebrafish, anatomical functional data from these suggest that this extends beyond system, exclusively retinal inputs. Consistent evolving view tectum, we...
Abstract Hearing is a crucial sense in underwater environments for communication, hunting, attracting mates, and detecting predators. However, the tools currently used to study hearing are limited, as they cannot controllably stimulate specific parts of auditory system. To date, contributions organs have been identified through lesion experiments that inactivate an organ, making it difficult gauge stimuli which each organ sensitive, or ways inputs from multiple combined during perception....
Abstract Phenotypic heterogeneity of cancer cells plays a critical role in shaping treatment response. This type is organized spatially with specific phenotypes, such as sharply demarcated clusters proliferating and cell cycle-arrested cells, predominating within discrete domains tumor. What determines the occurrence tumor phenotypes distinct microdomains solid cancers poorly understood. Here, we show that melanoma spatial organization phenotypic dictated by expression activity MITF. We...
Animals from insects to humans perform visual escape behavior in response looming stimuli, and these responses habituate if looms are presented repeatedly without consequence. While the basic processing motor pathways involved this have been described, many of nuances predator perception sensorimotor gating not. Here, we performed both behavioral analyses brain-wide cellular-resolution calcium imaging larval zebrafish while presenting them with loom stimuli or that selectively deliver either...
Abstract Zebrafish have a remarkable capacity to regenerate following spinal cord (SC) injury but the responsible cellular events are not well understood. We used in vivo imaging and genetics pin-point specific processes controlling SC regeneration zebrafish. identified two temporally mechanistically distinct phases of SC. The initial phase relies on migration precursor neurons injury, enabling rapid functional recovery, activation quiescent neural progenitor cells (NPCs). A second...
This study develops an original co-infection model in mice using Brucella melitensis, the most frequent cause of human brucellosis, and Trypanosoma brucei, agent African trypanosomiasis. Although immunosuppressive effects T. brucei natural hosts models are well established, we observed that injection chronically infected with B. melitensis induces a drastic reduction number spleen, main reservoir infection. Similar results obtained abortus- suis-infected melitensis-infected co-infected...