A5035962915- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Zebrafish Biomedical Research Applications
- Single-cell and spatial transcriptomics
- Digital Imaging for Blood Diseases
- Immune Cell Function and Interaction
- Per- and polyfluoroalkyl substances research
- Cell Image Analysis Techniques
- Immune Response and Inflammation
- Air Quality and Health Impacts
- Effects and risks of endocrine disrupting chemicals
- Diabetes and associated disorders
- Atmospheric chemistry and aerosols
- Hallucinations in medical conditions
- Proteoglycans and glycosaminoglycans research
- Phagocytosis and Immune Regulation
- Neuropeptides and Animal Physiology
- Immune responses and vaccinations
- Receptor Mechanisms and Signaling
- Toxic Organic Pollutants Impact
North Carolina State University
2023-2024
Université Libre de Bruxelles
2017-2024
Neuroscience Institute
2020
Walloon Excellence in Lifesciences and Biotechnology
2018
The liver is the largest solid organ in body, yet it remains incompletely characterized. Here we present a spatial proteogenomic atlas of healthy and obese human murine combining single-cell CITE-seq, single-nuclei sequencing, transcriptomics, proteomics. By integrating these multi-omic datasets, provide validated strategies to reliably discriminate localize all hepatic cells, including population lipid-associated macrophages (LAMs) at bile ducts. We then align this across seven species,...
Microglia, the tissue-resident macrophages of CNS, represent major targets for therapeutic intervention in a wide variety neurological disorders. Efficient reprogramming protocols to generate microglia-like cells vitro using patient-derived induced pluripotent stem will, however, require precise understanding cellular and molecular events that instruct microglial cell fates. This remains challenge since developmental origin microglia during embryogenesis is controversial. Here, genetic...
The mononuclear phagocytic system consists of many cells, in particular macrophages, scattered throughout the body. However, there is increasing evidence for heterogeneity tissue-resident leading to a pressing need new tools discriminate subsets from other hematopoietic lineages. Macrophage-expressed gene (Mpeg)1.1 an evolutionary conserved encoding perforin-2, pore-forming protein associated with host defense against pathogens. Zebrafish mpeg1.1:GFP and mpeg1.1:mCherry reporters were...
Per- and polyfluoroalkyl substances (PFASs) are used in a multitude of processes products, including nonstick coatings, food wrappers, fire-fighting foams. These chemicals environmentally-persistent, ubiquitous, can be detected the serum 98% Americans. Despite evidence that PFASs alter adaptive immunity, few studies have investigated their effects on innate immunity. The report here presents results impact nine environmentally-relevant [e.g. perfluorooctanoic acid (PFOA), perfluorooctane...
Macrophages derive from multiple sources of hematopoietic progenitors. Most macrophages require colony-stimulating factor 1 receptor (CSF1R), but some persist in the absence CSF1R. Here, we analyzed
In vertebrates, the ontogeny of microglia, resident macrophages central nervous system, initiates early during development from primitive macrophages. While murine embryonic microglia then persist through life, in zebrafish these cells are transient, as they fully replaced by an adult population originating larval hematopoietic stem cell (HSC)-derived progenitors. Colony-stimulating factor receptor 1 (csf1r) is a fundamental regulator including which possess two paralogous genes: csf1ra and...
Recent studies have highlighted the heterogeneity of immune cell compartment within steady-state murine and human CNS. However it is not known whether this diversity conserved among non mammalian vertebrates, especially in zebrafish, a model system with increasing translational value. Here, we reveal complexity landscape adult zebrafish brain. Using single-cell transcriptomics, characterized these different subpopulations, including types that been -or poorly-characterized so far. By...
ABSTRACT Recent studies have highlighted the heterogeneity of immune cell compartment within steady-state murine and human CNS. However it is not known whether this diversity conserved among non mammalian vertebrates, especially in zebrafish, a model system with increasing translational value. Here, we reveal complexity landscape adult zebrafish brain. Using single-cell transcriptomics, characterized these different subpopulations, including types that been -or poorly-characterized so far....
Abstract The liver is the largest solid organ in body, yet it remains incompletely characterized. Here, we present a spatial proteogenomic atlas of healthy human and murine combining single-cell CITE-seq, single-nuclei sequencing, transcriptomics proteomics. By integrating these multi-omic datasets, provide validated strategies to reliably discriminate localize all hepatic cells. We then align this across seven species, revealing conserved program bona fide Kupffer cells bile-duct...
ABSTRACT In vertebrates, the ontogeny of microglia, resident macrophages central nervous system, initiates early during development from primitive macrophages. While murine embryonic microglia then persist through life, in zebrafish these cells are transient, as they fully replaced by an adult population originating larval hematopoietic stem cell (HSC)-derived progenitors. Colony-stimulating factor receptor 1 (csf1r) is a fundamental regulator including which possess two paralogous genes:...
Recent studies have highlighted the heterogeneity of immune cell compartment within steady-state murine and human CNS. However it is not known whether this diversity conserved among non mammalian vertebrates, especially in zebrafish, a model system with increasing translational value. Here, we reveal complexity landscape adult zebrafish brain. Using single-cell transcriptomics, characterized these different subpopulations, including types that been -or poorly-characterized so far. By...
Normal development of the immune system is essential for overall health and disease resistance. Bony fish, such as zebrafish (Danio rerio), possess all major cell lineages mammals can be employed to model human host response challenge. Zebrafish neutrophils, example, are present in transparent larvae early 48 hours post fertilization have been examined numerous infection immunotoxicology reports. One significant advantage ability affordably generate high numbers individual that arrayed...
ABSTRACT The mononuclear phagocytic system (MPS) consists of many cells, in particular macrophages, scattered throughout the body. However, there is increasing evidence for heterogeneity tissue-resident leading to a pressing need new tools discriminate MPS subsets from other hematopoietic lineages. Mpeg1.1 an evolutionary conserved gene encoding perforin-2 , pore-forming protein associated with host defense against pathogens. Zebrafish mpeg1.1:GFP and mpeg1.1:mCherry reporters were...
Event Abstract Back to Embryonic microglia derive from primitive macrophages and are replaced by cmyb-dependent definitive in zebrafish Giuliano Ferrero1, 2*, Chris Mahony3, 4, Eleonore Dupuis1, 2, Laurent Yvernogeau5, Elodie Di Ruggiero1, Magali Miserocchi1, Marianne Caron1, Catherine Robin5, David Traver6, Julien Y. BERTRAND4 Valerie Wittamer1, 2* 1 Free University of Brussels, Belgium 2 Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire, 3 Université Genève,...
Event Abstract Back to TGF-ß receptor I promotes early microglia development in zebrafish embryo Giuliano Ferrero1* and Valerie Wittamer1 1 ULB, IRIBHM, Belgium Because neurodegenerative diseases are often associated with neuroinflammation, microglia, the resident macrophages central nervous system, represent a potential target for of novel therapeutic approaches. Our research aims at characterizing ontogeny, an important aspect biology that remains poorly understood. first steps ontogeny...
Normal development of the immune system is essential for overall health and disease resistance. Bony fish, such as zebrafish (
Abstract Macrophages derive from multiple sources of hematopoietic progenitors. Most macrophages require colony-stimulating factor 1 receptor (CSF1R), but some persist in the absence CSF1R. Here, we analyzed mpeg1 :GFP–expressing csf1r -deficient zebrafish and report that embryonic emerge followed by their developmental arrest. In larvae, + cell numbers then increased showing two distinct types skin: branched, putative Langerhans cells, amoeboid cells. contrast, although also -mutants,...