A5005628118- Veterinary Equine Medical Research
- Asthma and respiratory diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Veterinary Practice and Education Studies
- Cell Adhesion Molecules Research
- Inhalation and Respiratory Drug Delivery
- Protease and Inhibitor Mechanisms
- Salmonella and Campylobacter epidemiology
- Cancer Genomics and Diagnostics
- Reproductive Physiology in Livestock
- Allergic Rhinitis and Sensitization
- Innovations in Medical Education
- Cellular Mechanics and Interactions
- Mechanical Circulatory Support Devices
- Human-Animal Interaction Studies
- Inflammasome and immune disorders
- Vibrio bacteria research studies
- Genetic factors in colorectal cancer
- Veterinary medicine and infectious diseases
- Sepsis Diagnosis and Treatment
- Extracellular vesicles in disease
- Effects of Environmental Stressors on Livestock
- Health Literacy and Information Accessibility
- Thermal Expansion and Ionic Conductivity
North Carolina State University
2014-2025
Friedrich-Alexander-Universität Erlangen-Nürnberg
2016
Per- and polyfluoroalkyl substances (PFASs) are used in a multitude of processes products, including nonstick coatings, food wrappers, fire-fighting foams. These chemicals environmentally-persistent, ubiquitous, can be detected the serum 98% Americans. Despite evidence that PFASs alter adaptive immunity, few studies have investigated their effects on innate immunity. The report here presents results impact nine environmentally-relevant [e.g. perfluorooctanoic acid (PFOA), perfluorooctane...
Growing evidence supports the importance of extracellular vesicle (EV) as mediators communication in pathological processes, including those underlying respiratory disease. However, establishing methods for isolating and characterizing EVs remains challenging, particularly samples. This study set out to address this challenge by comparing different EV isolation evaluating their impacts on yield, markers purity, proteomic signatures, utilizing equine/horse bronchoalveolar lavage Horses can...
Neutrophil extracellular traps (NETs) play a significant role in response to variety of infectious and inflammatory stimuli human veterinary medicine. Although entrapment bacteria can be an important function NETs, the exuberant release DNA other intracellular molecules has also been negatively implicated pathogenesis different diseases. Thus, NET formation must tightly controlled represents opportunity for therapeutic interventions. Horses are particularly sensitive bacterial that have...
Equine asthma syndrome (EAS) is a common problem that affects horses of any age. Severe EAS reported to affect 10-20% adult in the northern hemisphere, while mild/moderate 60-100% horses, depending on population and geographic region. For both severe EAS, presence lower airway inflammation attributed airborne "triggers" such as dust, mold bacterial components encounter hay stable-environments; treatment recommendations for with often include full-time pasture turnout. The caveat this...
Plasma cytokine adsorption has shown benefit as an adjunctive therapy in human sepsis but yet to be investigated horses. We hypothesized that ex vivo filtration of equine plasma with a novel device would significantly reduce concentrations lipopolysaccharide-stimulated cytokines. also the adsorb medications commonly used treat sepsis.
Develop, implement, and monitor for adverse effects of, a novel hemoperfusion therapy in adult horses.
Cell-free DNA (cfDNA) is fragmented extracellular that released from cells into various body fluids. Previously published data adult horses supports cfDNA as a potential disease biomarker, but also shows direct measurement in plasma inaccurate due to matrix effect. It currently unknown whether similar effect exists foal plasma. Given this, the objectives of current study were investigate for during fluorescence using Qubit fluorometer, and determine neat and/or extracted concentrations are...
The post operative response of the large colon wall after a surgically corrected volvulus (LCV) has not been investigated.To use transabdominal ultrasound to monitor change in thickness following surgical correction LCV.A prolonged period involution is correlated with an increased rate morbidity and mortality.A prospective clinical study including horses that presented North Carolina State University Veterinary Teaching Hospital for colic between September 2006 March, 2008, had diagnosed LCV...
In many equine inflammatory disease states, neutrophil activities, such as adhesion, migration, and reactive oxygen species (ROS) production become dysregulated. Dysregulated activation causes tissue damage in horses with asthma, colitis, laminitis, gastric glandular disease. Non-steroidal anti-inflammatory drugs do not adequately inhibit functions can lead to dangerous adverse effects. Therefore, novel therapies that target mechanisms of neutrophil-mediated are needed. One potential...
Equine asthma is a naturally occurring lung disease characterized by chronic, partially reversible airway obstruction, pulmonary remodeling, and lower inflammation. Asthma currently divided into two major groups, mild to moderate (mEA) severe (sEA), but further subtyping phenotype (i.e., clinical presentation) and/or endotype cellular mechanisms) may be warranted. For this study, we were interested in investigation of inflammatory characteristics EA, including mast cells. The purpose study...
An 8-year-old American Quarter Horse gelding was treated with extracorporeal hemoperfusion (HP) therapy for treatment of Clostridioides difficile (C. difficile) colitis-induced systemic inflammatory response syndrome (SIRS). The developed C. associated peracute colitis and severe SIRS as evidenced by a positive fecal PCR tachypnea, tachycardia, fever, neutropenia, altered mucous membrane color, hyperlactatemia. Concurrent acute kidney injury in the horse limited use routine anti-inflammatory...
Myristoylated alanine-rich C-kinase substrate (MARCKS) is a ubiquitously expressed of protein kinase C (PKC) that involved in reorganization the actin cytoskeleton. We hypothesized MARCKS regulation fibroblast migration and addressed this hypothesis by utilizing unique reagent developed laboratory, MANS peptide. The peptide myristoylated cell permeable corresponding to first 24-amino acids inhibits function. Treatment NIH-3T3 fibroblasts with attenuated scratch wounding assays, while...
Neutrophils are innate immune response cells designed to kill invading microorganisms. One of the mechanisms neutrophils use bacteria is generation damaging reactive oxygen species (ROS) via respiratory burst. However, during enteric salmonellosis, neutrophil-derived ROS actually facilitates Salmonella expansion and survival in gut. This seeming paradox led us hypothesize that may possess influence neutrophil In this work, we used an vitro Salmonella-neutrophil co-culture model examine...
Pro-inflammatory cytokines including tumor necrosis factor α (TNFα), IL-1β, IL-6, and IL-8 are potent immune mediators that exacerbate multiple equine diseases such as sepsis laminitis. Unfortunately, safe effective cytokine-targeting therapies lacking in horses; therefore, novel mechanisms of inhibiting cytokine production critically needed. One potential mechanism for synthesis is elevation intracellular cyclic AMP (cAMP). In human leukocytes, cAMP induced by activation E-prostanoid (EP)...