A5068729906- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Fungal and yeast genetics research
- Viral gastroenteritis research and epidemiology
- Bacterial Genetics and Biotechnology
- Microbial Metabolism and Applications
- Enzyme Production and Characterization
- Bacterial biofilms and quorum sensing
- Enzyme Structure and Function
- Mitochondrial Function and Pathology
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Microbial Natural Products and Biosynthesis
- Clostridium difficile and Clostridium perfringens research
- Cancer Research and Treatments
- DNA Repair Mechanisms
- Polyamine Metabolism and Applications
- Microtubule and mitosis dynamics
- Gut microbiota and health
- Enzyme Catalysis and Immobilization
- Genomics and Chromatin Dynamics
- Antimicrobial Peptides and Activities
Marshall University
2020-2024
Texas A&M University
2005-2022
Kazan Federal University
2010-2021
Texas A&M Health Science Center
2015-2020
Texas A&M University System
2008-2019
Bryan College
2014-2018
Institute of Chemical Biology and Fundamental Medicine
2018
National Institute of Diabetes and Digestive and Kidney Diseases
2008
Mitchell Institute
2006
We constructed two collections of targeted single gene deletion (SGD) mutants and multi-gene (MGD) in Salmonella enterica sv Typhimurium 14028s. The SGD mutant contain (1), 3517 which a is replaced by cassette containing kanamycin resistance (KanR) oriented the sense direction (SGD-K), (2), 3376 with chloramphenicol (CamR) antisense (SGD-C). A combined total 3773 individual genes were deleted across these collections. MGD bearing deletions contiguous regions three or more include (3), 198...
Multidrug efflux pumps are integral membrane proteins known to actively excrete antibiotics. The macrolide-specific pump MacAB, the only ABC-type drug in Salmonella, has previously been linked virulence mice. molecular mechanism of this link between macAB and infection is unclear. We demonstrate that plays a role detoxification reactive oxygen species (ROS), compounds salmonellae exposed at various stages infection. induced upon exposure H2O2 critical for survival Salmonella enterica serovar...
Salmonella enterica subspecies I serotypes are responsible for the vast majority of salmonellosis in mammals and birds, yet only a few factors specific to this group that allow them persist niche have been identified. We show STM0557, S. I-specific gene encoding an inner membrane protein, is critical faecal shedding intestinal persistence serotype Typhimurium ATCC14028 Salmonella-resistant mice, but mutations do not diminish short-term colonization or invasion cultured epithelial cells....
Salmonella enterica serotype Typhimurium can move through liquid using swimming motility, and across a surface by swarming motility. We generated library of targeted deletion mutants in strain ATCC14028, primarily genes specific to Salmonella, that we have previously described. In the work presented here, screened each individual mutant from this for ability away site inoculation on motility agar. Mutants described as important such flgF, motA, cheY are do not plates our screens, validating...
Serratia marcescens is an emerging opportunistic pathogen responsible for many hospital-acquired infections including catheter-associated bacteremia and urinary tract respiratory infections. Biofilm formation one of the mechanisms employed by S. to increase its virulence pathogenicity. Here, we have investigated main steps biofilm SR 41-8000. It was found that growth stimulated nutrient-rich environment. The time-course experiments showed cells adhere surface catheter start produce...
Nontyphoidal Salmonella bacteria induce a classic inflammatory diarrhea by eliciting large influx of neutrophils, producing robust oxidative burst. Despite substantial progress understanding the benefits to host response , little is known regarding how can simultaneously resist damaging effects The multidrug efflux pump MacAB important for survival stress both in vitro and during infection. We describe new pathway used Typhimurium detoxify extracellular reactive oxygen species using (MacAB)...
The opportunistic pathogen Serratia marcescens can cause urinary tract infections, respiratory meningitis, and sepsis in immunocompromised individuals. These infections are challenging to treat due the intrinsic resistance of S. an extensive array antibiotics.
Salmonellosis is one of the most important bacterial food borne illnesses worldwide. A major source infection for humans consumption chicken or egg products that have been contaminated with Salmonella enterica serotype Typhimurium, however our knowledge regarding colonization and persistence factors in small.We compared intestinal systemic 1-week-old White Leghorn chicks Salmonella-resistant CBA/J mice during Typhimurium ATCC14028, commonly studied isolates. We also distribution wild type...
Coordination between cellular metabolism and DNA replication determines when cells initiate division. It has been assumed that only plays a permissive role in cell While blocking arrests division, it is not known whether an up-regulation of metabolic reactions accelerates cycle transitions. Here, we show increasing the amount mitochondrial overall proliferation promotes nuclear replication, nutrient-dependent manner. The Sir2p NAD+-dependent de-acetylase antagonizes this role. We found with...
TatC (STM3975) is a highly conserved component of the Twin Arginine Transport (Tat) systems that required for transport folded proteins across inner membrane in gram-negative bacteria. We previously identified ΔtatC mutant as defective competitive infections with wild type ATCC14028 during systemic infection Salmonella-susceptible BALB/c mice. Here we confirm these results and show internalized poorly by cultured J774-A.1 mouse macrophages phenotype may be related to defect. This also...
ABSTRACT Cattle are naturally infected with Salmonella enterica serotype Typhimurium and exhibit pathological features of enteric salmonellosis that closely resemble those in humans. the most relevant model gastrointestinal disease resulting from nontyphoidal infection an animal intact microbiota. We utilized this to screen a library targeted single-gene deletion mutants identify novel genes required for survival during infection. Fifty-four candidate were strongly selected, including...
How proliferating cells maintain the copy number and overall size of their organelles is not clear. We had previously reported that in budding yeast Saccharomyces cerevisiae G1 cyclin Cln3p required for vacuolar (lysosomal) homotypic fusion loss leads to fragmentation. The Cdc42p GTPase also vacuole fusion. Here we show scaffold protein Bem1p, a critical regulator activity, downstream effector cyclin-dependent kinase (Cdk) Cdc28p. Our results suggest Bem1p phosphorylated Cdk-dependent manner...
Multicopy single-stranded DNAs (msDNAs) are hybrid RNA-DNA molecules encoded on retroelements called retrons and produced by the action of retron reverse transcriptases. Retrons widespread in bacteria but natural function msDNA has remained elusive despite 30 years study. The major roadblock to elucidation these unique been lack any identifiable phenotypes for mutants unable make msDNA. We report that zoonotic pathogen Salmonella Typhimurium is necessary colonization intestine. Similarly, we...
Plants synthetize a large spectrum of secondary metabolites with substantial structural and functional diversity, making them rich reservoir new biologically active compounds. Among different plant lineages, the evolutionarily ancient branch non-vascular plants (Bryophytes) is particular interest as these organisms produce many unique compounds highly promising antibacterial properties. Here, we characterized activity produced by ecotypes (strains) model mosses Physcomitrium patens Sphagnum...
Neutrophils are innate immune response cells designed to kill invading microorganisms. One of the mechanisms neutrophils use bacteria is generation damaging reactive oxygen species (ROS) via respiratory burst. However, during enteric salmonellosis, neutrophil-derived ROS actually facilitates Salmonella expansion and survival in gut. This seeming paradox led us hypothesize that may possess influence neutrophil In this work, we used an vitro Salmonella-neutrophil co-culture model examine...
pyrE (STM3733) encodes orotate phosphoribosyltransferase (OPRTase; EC 2.4.2.10), the fifth enzyme of de novo pyrimidine biosynthetic pathway. We identified a ΔpyrE mutant as under selection in screening Salmonella library 4-day old chicks. Here, we confirm that colonizes chicks poorly competitive infection with isogenic wild type, and ability this to colonize could be restored by providing copy trans. further show our grows nutrient poor conditions vitro, grow is restored, both vitro chicks,...
Non-subspecies I salmonellae are commensals of cold-blooded vertebrates and cause sporadic disease in mammals. The reasons why non-subspecies do not circulate populations warm-blooded vertebrates, but instead only occasional this niche, unknown. We examined the ability Salmonella enterica subsp. IIIa (subsp. arizonae) IIIb diarizonae) isolates to grow competitively with subspecies (serovar Typhimurium) ATCC 14028 vitro, colonize Salmonella-sensitive BALB/c mice, persist intestine...
Salmonella Typhimurium gene STM2215 (rtn) is conserved among many enterobacteriaceae. Mutants lacking poorly colonized the liver and spleen in intraperitoneal infection of mice intestine deeper tissues oral infection. These phenotypes were complemented by a wild-type copy provided trans. deletion mutants grew normally J774A.1 murine macrophages but unable to invade Caco-2 colonic epithelial cells. Consistent with this finding, produced lower levels effectors TTSS-1. predicted c-di-GMP...
Salmonella enterica serovar Enteritidis is a common cause of foodborne illness in the United States. The bacterium can be transmitted to humans via contaminated chicken meat and eggs, virulence requires type III secretion system 1 (TTSS-1), encoded on pathogenicity island (SPI-1). Chickens often carry S subclinically, obscuring role SPI-1 facilitating bacterial colonization. To evaluate infection chicks by Salmonella, we created utilized strains harboring stable fluorescent reporter fusion...
Salmonellae are pathogenic bacteria that cause significant morbidity and mortality in humans worldwide. establish infection avoid clearance by the immune system mechanisms not well understood. We previously showed l-asparaginase II produced Salmonella enterica serovar Typhimurium (S Typhimurium) inhibits T cell responses mediates virulence. In addition, we asparagine deprivation such as mediated of S causes suppression activation-induced metabolic reprogramming. Here, report STM3997, which...