A5102857347- Salmonella and Campylobacter epidemiology
- Bacterial Identification and Susceptibility Testing
- Gut microbiota and health
- Veterinary medicine and infectious diseases
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Animal Nutrition and Physiology
- Probiotics and Fermented Foods
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Mycobacterium research and diagnosis
- Dermatology and Skin Diseases
- Antibiotics Pharmacokinetics and Efficacy
- Prion Diseases and Protein Misfolding
- Bird parasitology and diseases
- Yersinia bacterium, plague, ectoparasites research
- Poxvirus research and outbreaks
- Helminth infection and control
- Endoplasmic Reticulum Stress and Disease
- Bacillus and Francisella bacterial research
- Drug Transport and Resistance Mechanisms
- Antimicrobial Resistance in Staphylococcus
- Autophagy in Disease and Therapy
- Urinary Tract Infections Management
University of Illinois Urbana-Champaign
2021-2025
Urbana University
2025
New York State College of Veterinary Medicine
2013-2019
Cornell University
2012-2019
To cause disease, Salmonella must invade the intestinal epithelium employing genes encoded within Pathogenicity Island 1 (SPI1). We show here that propionate, a fatty acid abundant in intestine of animals, repressed SPI1 at physiologically relevant concentration and pH, reducing expression transcriptional regulators consequently decreasing secretion effector proteins, leading to reduced bacterial penetration cultured epithelial cells. Essential repression was hilD, which occupies apex...
The emergence of antimicrobial resistance (AMR) in dogs constitutes a threat to animal and human health. There is lack studies Illinois that evaluated the prevalence AMR among urinary bacterial pathogens. In study, we included 803 isolates (299 Gram-positive 504 Gram-negative) were isolated from 2,583 canine urine samples submitted Veterinary Diagnostic Laboratory, University between 2019 2020 suspected tract infections (UTI). most common Staphylococcus pseudintermedius (17.93%),...
Abstract Objective To develop and validate a quantitative PCR (qPCR) assay for detecting Macrorhabdus ornithogaster (MO) reproducibly culture MO from infected budgerigars ( Melopsittacus undulatus ). Methods A TaqMan qPCR targeting 94-bp segment of 18S rRNA was evaluated limit detection, dynamic range, intra-assay variability, interassay efficiency. Proventricular-ventricular samples feces deceased positive on cytology were plated with Basal Medium Eagle or chicken serum media, 20% fetal...
ABSTRACT Salmonella spp. are carried by and can acutely infect agricultural animals humans. After ingestion, salmonellae traverse the upper digestive tract initiate tissue invasion of distal ileum, a virulence process out type III secretion system encoded within pathogenicity island 1 (SPI-1). Salmonellae coordinate SPI-1 expression with anatomical location via environmental cues, one which is bile, complex fluid that causes potent repression genes. The individual components bile responsible...
This report documents the exposure of passengers and crew a commercial international flight to zoonotic pathogen Brucella canis after an infected dog aborted in passenger cabin aircraft. case demonstrates challenges associated with brucellosis screening risks that airline personnel, airport employees travellers face when animals unrecognized infections are transported.
Abstract Frequent incidence of postweaning enterotoxigenic Escherichia coli (ETEC) diarrhea in the swine industry contributes to high mortality rates and associated economic losses. In this study, a combination butyric, caprylic, capric fatty acid monoglycerides was investigated promote intestinal integrity host defenses weanling pigs infected with ETEC. A total 160 were allotted treatment groups based on weight sex. Throughout 17-d three maintained: sham-inoculated fed control diet...
Virulence functions of bacterial pathogens are often energetically costly and thus subjected to intricate regulatory mechanisms. In Salmonella, invasion the intestinal epithelium, an essential early step in virulence, requires production a multi-protein type III secretion apparatus. The pathogen mitigates overall cost by inducing it only fraction its population. This constitutes successful virulence strategy as small number is sufficient promote proliferation non-invading majority. Such...
Salmonella pathogenicity island 1 (SPI1), comprising a type III section system that translocates effector proteins into host cells, is essential for the enteric pathogen to penetrate intestinal epithelium and subsequently cause disease. Using random transposon mutagenesis, we found Tn10 disruption in flagellar fliDST operon induced SPI1 expression when strain was grown under conditions designed repress SPI1, by mimicking environment of large intestine through use fatty acid butyrate. Our...
Salmonella enterica serovar Enteritidis is a common cause of foodborne illness in the United States. The bacterium can be transmitted to humans via contaminated chicken meat and eggs, virulence requires type III secretion system 1 (TTSS-1), encoded on pathogenicity island (SPI-1). Chickens often carry S subclinically, obscuring role SPI-1 facilitating bacterial colonization. To evaluate infection chicks by Salmonella, we created utilized strains harboring stable fluorescent reporter fusion...
N-Lysine acylation is a post-translational modification important for both prokaryotic and eukaryotic cells to control wide array of cellular functions. Here we demonstrate that the protein acyltransferase Pat regulates genes on Salmonella Pathogenicity Island 1 (SPI1) are required invasion intestinal epithelium. Mutation pat slightly increased spleen colonization by in streptomycin-treated mice, with more mutant reaching than wild type strain. Growth under specific conditions selectively...
Nitrofurantoin, a broad-spectrum nitrofuran class antibiotic, is applied as first-line antibiotic in treating human urinary tract infections (UTIs) due to its great efficacy and high achievable concentration. The interest using this companion animals has increased the growing demand for effective antibiotics treat UTIs caused by multidrug-resistant bacteria. Currently, susceptibility interpretations nitrofurantoin are based on breakpoints set humans, while canine-specific still unavailable....
Campylobacter hyointestinalis was initially isolated from an asymptomatic black howler monkey (Alouatta caraya) in a routine fecal culture examination. Fecal cultures other individuals this group and adjacently housed black-headed spider (Ateles fusciceps robustus) recovered C. all but one of the sampled (1.1 monkeys 2.1 monkeys). Concurrently, presented with acute onset severe rectal prolapse diarrhea. Whole-genome sequencing results isolates were homologous closely related to subsp....
Abstract Aims Judicious antimicrobial use in companion animal practice is critical for maintaining the effectiveness of agents against bacterial infections and reducing selection antimicrobial‐resistant bacteria. This study aimed to provide insights into veterinarians' treatment recommendations common dogs cats describe factors influencing their prescription choices. Methods Results An online survey using QualtricsXM® software was administered between September November 2022 veterinarians...
The objectives of this study were to describe the clinical features cats diagnosed with tularemia, and compare without tularemia identify characteristics increasing likelihood a diagnosis.
Two aborted Chester White pig fetuses were presented to a veterinary diagnostic laboratory in Illinois. Postmortem examination identified no gross abnormalities. Histologic evaluation revealed multifocal necrosis of chorionic epithelial cells, coalescing areas mineralization the placenta, and focal accumulations viable degenerate neutrophils lung. Intra- extracellular acid-fast bacilli lesions both placenta lungs. Bacterial culture stomach contents yielded heavy growth Mycobacterium...
We developed a rapid insulated isothermal PCR (iiPCR) assay for on-site detection of Mannheimia haemolytica using primer and probe set targeting the superoxide dismutase (sodA) gene. Our iiPCR detected M. clinical isolates successfully produced negative results on other bovine or ovine respiratory pathogens, including Histophilus somni, Bibersteinia trehalosi, Trueperella pyogenes, Streptococcus suis, Mycoplasma spp., indicating that reactions were specific. Additionally, our as few 21...
Abstract Frequent incidence of post-weaning enterotoxigenic Escherichia coli (ETEC) diarrhea in the swine industry contributes to high mortality rates and associated economic losses. In this study, effects butyric, caprylic, capric monoglycerides (MG) were investigated as a way promote intestinal integrity host defenses weanling pigs infected with ETEC. A total 160 equal sex allotted treatment groups based on body weight sex. Throughout 17-d three maintained: sham-inoculated fed control diet...