A5074035213- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Probiotics and Fermented Foods
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- Aquaculture disease management and microbiota
- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Inflammasome and immune disorders
- Genomics, phytochemicals, and oxidative stress
- Molecular Biology Techniques and Applications
- Respiratory viral infections research
- RNA Interference and Gene Delivery
- Milk Quality and Mastitis in Dairy Cows
- Viral Infections and Vectors
- Salmonella and Campylobacter epidemiology
- Blood groups and transfusion
- Antimicrobial Peptides and Activities
- Heme Oxygenase-1 and Carbon Monoxide
- Plant Virus Research Studies
- Microbial Metabolites in Food Biotechnology
- Veterinary Oncology Research
Southwest Minzu University
2008-2025
Sichuan University
2006-2022
Qinghai Tibetan Hospital
2022
Case Western Reserve University
2019
University School
2019
Sichuan Academy Of Social Sciences
2012
Southwest University
2005
West China Medical Center of Sichuan University
2005
Due to huge economic losses the dairy industry worldwide, mastitis can be considered as one of most common diseases in cows. This work aimed study this disease by comparing multiple biological specimens (feces, serum, and urine) from individuals with or without clinical mastitis. was performed a single analytical platform, namely 1H-NMR, through multi-matrix strategy. Thanks high reproducibility we could characterize 120 molecules across cow feces, urine. Among them, 23 were three biofluids....
Abstract Background Bopivirus B is an emerging picornavirus that affects goats in China. This study aimed to establish a TaqMan real-time PCR assay for detecting and conduct preliminary survey of infection six goat farms Sichuan province, Specific primers probe targeting the 3D gene were designed, TaqMan-based was successfully established following optimization reaction conditions components. A total 257 fecal samples collected from tested using newly developed method. Results The...
MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs that regulate target gene expression in many eukaryotes. MiRNAs essential for post-transcriptional regulation, influencing various biological functions, including oocyte growth and maturation, fertilization, early embryo development, implantation. In recent decades, numerous studies have identified substantial number miRNAs associated with mammalian maturation utilizing methods such as RNA sequencing modulating miRNA through...
Ammonia poses a significant challenge in the contemporary intensive breeding industry, resulting substantial economic losses. Despite this, there is dearth of research investigating efficacious strategies to prevent ammonia poisoning poultry. Consequently, objective this study was investigate molecular mechanisms through which Luteolin (Lut) safeguards mitochondria and restores equilibrium energy metabolism disorders, thereby shielding chicken spleen lymphocytes from detrimental effects...
Abstract Background The selection of stably expressed reference genes is a prerequisite when evaluating gene expression, via real-time PCR, in cells response to viral infections. objective our study was identify suitable for mRNA expression analysis chicken embryonic fibroblasts (CEF) after infection with avian leukosis virus subgroup J (ALV-J). Findings levels 11 potential CEF infected ALV-J were determined by PCR. stability these analyzed and ranked using the geNorm tool. Analysis...
Crohn's disease (CD) is a chronic inflammatory bowel (IBD) of the digestive tract in humans. There evidence that Parabacteroides distasonis could contribute to IBD. Here, we present complete genome sequence strain designated CavFT-hAR46, which was isolated from gut intramural cavernous fistulous (CavFT) microlesion CD patient.
Mycoplasma ovipneumoniae is associated with chronic nonprogressive pneumonia in both sheep and goats. Studies concerning its molecular pathogenesis, genetic analysis, vaccine development have been hindered due to limited genomic information. Here, we announce the first complete genome sequence of this organism.
A real-time PCR for detection and quantification of M. ovipneumoniae was developed using 9 recently sequenced genomes primers targeting a putative adhesin gene p113. The assay proved to be specific sensitive (with limit 22 genomic DNA) could quantify DNA over wide linear range, from 2.2 × 102 107 genomes.
The hydrophilic polysaccharides (PS) were isolated and purified from the tuberous roots of Pseudostellaria heterophylla. extraction process PS Pesudostellariae radix was optimized by single–factor experiments orthogonal design. extract DEAE cellulose column to obtain pure polysaccharide PHP. Then PHP treated with different intensities sonication study effect on PHP’s characteristics its biological activity in vitro vivo. results this revealed that ultrasound treatment did not significantly...
Duck enteritis virus (DEV) is a herpesvirus that causes an acute, contagious, and fatal disease. In the present article, we introduce quantitative real-time polymerase chain reaction (PCR) assay for DEV DNA using TaqMan technology two-step protocol. It was confirmed to be rapid, sensitive, specific detection. The primers probe were designed directed gene of DEV. method will provide valuable tool rapid laboratory diagnosis infection. By virtue its high-throughput format ability accurately...
Mastitis is commonly recognized as a localized inflammatory udder disease induced by the infiltration of exogenous pathogens. In present study, our objective was to discern fecal and milk variations in both microbiota composition metabolite profiles among three distinct groups cows: healthy cows, cows with subclinical mastitis clinical mastitis. The microbial community significantly less rich diverse than one harbored cows. parallel, caused strong disturbance microbiota. Metabolomic showed...
Duck hepatitis A virus genotype C (DHAV-C), recognized recently, is one of the pathogens causing fatal duck viral in ducklings, especially Asia. To demonstrate pathogenesis DHAV-C isolate, 3-day-old specific pathogen free ducklings were inoculated subcutaneously with a isolate and clinical signs observed. Virus distribution, histological apoptotic morphological changes various tissues examined at different times post inoculation. The serial, characteristic included haemorrhage swelling...
Enterotoxigenic Escherichia coli (ETEC) can cause intestinal inflammation and diarrhea in yaks, which has a negative impact on their economic value. In recent years, probiotics have gained increasing attention as pure, natural, nontoxic, harmless, residue-free additive. However, the underlying mechanisms by safeguard against ETEC are not completely elucidated. This study aimed to investigate protective effect of Enterococcus faecium ( E. faecium) infection mice through oral gavage....
A metavirome analysis was performed and detected bopivirus in the diarrhoeal fecal samples of goats China. total 136 were collected from yeanlings between dates June 2021 January 2022 Sichuan province, Moreover, “Bopivirus B” strains by a specific RT-PCR targeting 3D gene virus. The results showed that overall detection rate 19.12% (26/136). Additionally, there higher (24.05%, 19/79) with diarrhea compared to those asymptomatic animals (12.28%, 7/57). In these samples, no other common...