A5028954136- Enterobacteriaceae and Cronobacter Research
- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Probiotics and Fermented Foods
- Identification and Quantification in Food
- Gut microbiota and health
- Bacterial Genetics and Biotechnology
- Pharmaceutical and Antibiotic Environmental Impacts
- Parasitic Infections and Diagnostics
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Antimicrobial agents and applications
- Antimicrobial Peptides and Activities
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Plant Pathogenic Bacteria Studies
- Aquaculture disease management and microbiota
- Folate and B Vitamins Research
- Molecular Biology Techniques and Applications
- Listeria monocytogenes in Food Safety
- bioluminescence and chemiluminescence research
- Peanut Plant Research Studies
- Antibiotic Use and Resistance
- Infant Nutrition and Health
United Arab Emirates University
2020-2025
Auburn University
2025
University College Dublin
2016-2022
Trinity College Dublin
2012-2016
Northwest A&F University
2016
University of Liverpool
2015
University of California, Irvine
2014
Karnataka Veterinary Animal and Fisheries Sciences University
2006-2012
Technical University of Munich
2010-2011
Leibniz-Institute for Food Systems Biology at the Technical University of Munich
2010-2011
Bacterial transcriptional networks consist of hundreds transcription factors and thousands promoters. However, the true complexity in a bacterial pathogen effect environments encountered during infection remain to be established. We present simplified approach for global promoter identification bacteria using RNA-seq-based transcriptomic analyses 22 distinct infection-relevant environmental conditions. Individual RNA samples were combined identify most 3,838 Salmonella enterica serovar...
Salmonella enterica serovar Typhimurium is arguably the world's best-understood bacterial pathogen. However, crucial details about genetic programs used by bacterium to survive and replicate in macrophages have remained obscure because of challenge studying gene expression intracellular pathogens during infection. Here, we report use deep sequencing (RNA-seq) reveal transcriptional architecture activity infection murine macrophages, providing new insights into strategies pathogen a...
We constructed two collections of targeted single gene deletion (SGD) mutants and multi-gene (MGD) in Salmonella enterica sv Typhimurium 14028s. The SGD mutant contain (1), 3517 which a is replaced by cassette containing kanamycin resistance (KanR) oriented the sense direction (SGD-K), (2), 3376 with chloramphenicol (CamR) antisense (SGD-C). A combined total 3773 individual genes were deleted across these collections. MGD bearing deletions contiguous regions three or more include (3), 198...
To study the antibiogram of 40 seafood isolates Salmonella and use PCR to detect presence integrons genes coding for antibiotic resistance.In this study, were used analysis. The multidrug-resistant analyzed integron using integron-specific primers. Twenty-five percentage multidrug resistant while 67·50% at least two antibiotics. Antibiotic resistance catA1 tetA present in 57·52 60%, respectively. Although widespread was observed, only 26·08% catA1-carrying exhibited phenotypic against...
Infections associated with antimicrobial-resistant bacteria now represent a significant threat to human health using conventional therapy, necessitating the development of alternate and more effective antibacterial compounds. Silver nanoparticles (Ag NPs) have been proposed as potential antimicrobial agents combat infections. A complete understanding their activity is required before these molecules can be used in therapy. Lysozyme coated Ag NPs were synthesized characterized by TEM-EDS,...
ABSTRACT This study investigates the stability and shelf life of lemongrass essential oil‐in‐water (LGEO/W) nanoemulsions, a rich source bioactive compounds (e.g., citral citronellal) promising natural preservative. Initially, LGEO/W emulsions were produced through premix membrane emulsification (PME) traditional methods such as high‐pressure homogenization (HPH) ultrasonication (US) to generate micron‐ nano‐sized emulsions. Subsequent investigations focused on assessing nanoemulsions under...
ABSTRACT Only three pathogenic bacterial species, Salmonella enterica , Clostridium perfringens and Listeria monocytogenes are able to utilize both ethanolamine 1,2-propanediol as a sole carbon source. Degradation of these substrates, abundant in food the gut, depends on cobalamin, which is synthesized de novo only under anaerobic conditions. Although eut pdu cob - cbi gene clusters comprise 40 kb, conditions they confer selection advantage food-borne pathogens remain largely unknown. Here...
Cronobacter sakazakii is a xerotolerant neonatal pathogen epidemiologically linked to powdered infant food formula, often resulting in high mortality rates. Here, we used transcriptome sequencing (RNA-seq) provide transcriptional insights into the survival of C. desiccated conditions. Our RNA-seq data show that about 22% total genes were significantly upregulated and 9% downregulated during desiccation survival. When reverse transcription-quantitative PCR (qRT-PCR) was validate data, found...
Abstract Of 150 Escherichia coli strains we cultured from specimens taken cattle in Europe, 3 had elevated MICs against colistin. We assessed all for the presence of plasmid-mediated mcr-1 gene and identified 1 isolate as mcr-1–positive co-resistant to β-lactam, florfenicol, fluoroquinolone antimicrobial compounds.
Antimicrobial efflux is one of the important mechanisms causing multi-drug resistance (MDR) in bacteria. Chemosensitizers like 1-(1-naphthylmethyl)-piperazine (NMP) can inhibit an pump and therefore overcome MDR. However, secondary effects NMP other than inhibition are rarely investigated. Here, using phenotypic assays, microarray transcriptomic assays we show that creates membrane destabilization MDR Klebsiella pneumoniae MGH 78578 strain. The mediated activity was measured β-Lactamase...
Antimicrobial resistance is a global health challenge. Few new antibiotics have been developed for use over the years, and preserving efficacy of existing compounds an important step to protect public health. This paper describes study that examines effects exogenously induced oxidative stress on K. pneumoniae uncovers target could be useful harness as strategy mitigate resistance.
The capability of Salmonella enterica serovar Typhimurium strain 14028 (S. 14028) to utilize myo-inositol (MI) is determined by the genomic island GEI4417/4436 carrying iol genes that encode enzymes, transporters, and a repressor responsible for MI catabolic pathway. In contrast all bacteria investigated thus far, S. growing on as sole carbon source characterized remarkable long lag phase 40 60 h. We report here solid medium with source, this human pathogen exhibits bistable phenotype...
Cronobacter sakazakii is a Gram-negative opportunistic pathogen that causes life- threatening infantile infections, such as meningitis, septicemia, and necrotizing enterocolitis, well pneumonia, urinary tract wound infections in adults. Here, we report 26 draft genome sequences of C. sakazakii, which were obtained from dried spices the USA, Middle East, China, Republic Korea. The average size genomes was 4393 kb, with an 4055 protein coding genes, G + C content 56.9%. contained genes related...
Bioactive peptides derived from milk proteins are widely known to possess antibacterial activities. Even though the effects of milk-derived characterized, not much focus is given their antifungal characterization. Therefore, in this study, we investigated properties camel and cow whey casein hydrolysates against various species pathogenic Candida. The were produced using 2 enzymes (alcalase protease) at differing hydrolysis durations (2, 4, 6 h) tested for properties. results showed that...
The factors influencing the virulence of P. aeruginosa in development invasive infection remain poorly understood. Here, we investigated role host microenvironment shaping pathogen and mechanisms involved. Comparing seven paired genetically indistingushable clinical bloodstream peripheral isolates aeruginosa, demonstrate that from microenvironments are more virulent compared to their counterparts (p=0.025). Bloodstream elicited similar NF-kB responses isolated human monocytes implicating...