A5032502171- Listeria monocytogenes in Food Safety
- Enterobacteriaceae and Cronobacter Research
- Bacterial biofilms and quorum sensing
- Microbial Inactivation Methods
- Essential Oils and Antimicrobial Activity
- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Food Safety and Hygiene
- Vibrio bacteria research studies
- Antimicrobial Resistance in Staphylococcus
- Viral gastroenteritis research and epidemiology
- Bacterial Identification and Susceptibility Testing
- Mycotoxins in Agriculture and Food
- Probiotics and Fermented Foods
- Plant Pathogenic Bacteria Studies
- Biofuel production and bioconversion
- Bee Products Chemical Analysis
- Bacteriophages and microbial interactions
- Parasitic Infections and Diagnostics
- Biosensors and Analytical Detection
- Insect and Pesticide Research
- Microbial bioremediation and biosurfactants
- Protein purification and stability
- Polysaccharides and Plant Cell Walls
Jordan University of Science and Technology
2016-2025
Houston Methodist
2023
Indiana University – Purdue University Indianapolis
2023
Indiana University School of Medicine
2023
Purdue University West Lafayette
2001-2006
University of Manitoba
1996-2000
Genome Prairie
1998
Cronobacter spp. (formerly Enterobacter sakazakii), are a group of Gram-negative pathogens that have been implicated as causative agents meningitis and necrotizing enterocolitis in infants. The linked to infant formula; however, they also isolated from wide range foods environmental samples. In this study, 233 samples food, formula environment were screened for the presence an attempt find its source. Twenty nine strains spices, herbs, foods, dust obtained household vacuum cleaners. Among 76...
Heat stress has a serious impact on nutrient digestion and absorption in broiler chickens. This study aimed to investigate the effects of chronic heat (CHS) mRNA expression digestive enzymes transporter genes thermally manipulated (TM) The evaluated encompassed pancreatic lipase, trypsin, amylase, maltase, alkaline phosphatase as well certain glucose (GLUT2, SGLT1), amino acid (y+LAT1, CAT1), fatty (FABP1, CD36) jejunal mucosa. Thermal manipulation was carried out at 39°C 65% relative...
The 104-kDa Listeria adhesion protein (LAP) in monocytogenes is involved binding to various mammalian cell lines. However, the receptor that interacts with LAP eukaryotic cells unknown. In this study, scanning immunoelectron microscopy qualitatively demonstrated greater capacity of wild-type (WT) L. strain (F4244) than a LAP-deficient mutant (KB208) Caco-2 cells. goal study was identification host for LAP. Using Western blot ligand overlay assay, we identified 58 kDa be putative from...
Background and Aim The global emergence of Acinetobacter baumannii is great concern, especially inside intensive care units (ICUs). This study investigated the prevalence, antibiotic resistance, biofilm formation genetic relatedness A. recovered from ICU patients in three major hospitals Jordan. Methods isolates included this were identified by detection bla OXA-51 gene, a multiplex PCR assay. Antibiotic susceptibility testing was performed using disk diffusion broth microdilution methods,...
Many microbial infections are caused by multiple species. However, little is known about the detailed interactions and mechanisms underlying competition between coexisting pathogens. Acinetobacter baumannii a major nosocomial pathogen that often involved in mixed infections. To date, few studies have focused on A . other common coinfectious microbes. In this study, we aimed to investigate interspecies among clinical A. isolates with Klebsiella pneumoniae , Staphylococcus aureus Pseudomonas...
ABSTRACT Adhesion is a crucial first step in Listeria monocytogenes pathogenesis. In this study, we examined how the adhesion properties of serotypes correlate with their invasion efficiencies cell culture model (Caco-2) and mouse model. characteristics all 13 L. (25 strains) were analyzed, which yielded three distinct groups ( P < 0.05) high-, medium-, low-level-adhesion profiles. The efficiency these strains invading Caco-2 line was produced two groups; however, overall correlation R 2...
Listeria adhesion protein (LAP) is an important factor in monocytogenes and interacts with its cognate receptor, mammalian heat shock 60 (Hsp60). The genetic identity of LAP was determined to be alcohol acetaldehyde dehydrogenase (Aad). A recombinant Escherichia coli strain expressing aad confirmed the involvement Aad Caco-2 cells. Binding kinetics (ka) (rLAP) Hsp60 examined a surface plasmon resonance sensor 5.35 × 108 M−1 s−1 it equivalent binding anti-Hsp60 antibody (ka=2.15 109 s−1)...
The purpose of this study was to determine the influence growth conditions and medium composition on cellulase enzyme production by Streptomyces sp. Production a strain (J2) detected cellulose agar (CA) after 4 days incubation at 28 °C that exhibited clear zone 22 mm around colony. Cellulase assayed measuring amount glucose liberated in μmol/ml/min using dinitrosalicylic acid assay method. highest crude activity (432 U/l) observed 3 pH 7 60 supplemented with 0.5% glucose, 0.2% starch, NH4CL....
A conductometric immunosensor was developed for the detection of one most common foodborne pathogens, Escherichia coli O157:H7 (E. O157:H7), by sensing. The sensor built based on a polyaniline/zinc oxide (PANI/ZnO) nanocomposite film spin-coated gold electrode. Then, it modified with monoclonal anti-E. antibody as biorecognition element. fabricated nanostructured able to quantify pathogens under optimal conditions, within 30 min, and showed good range from 101 104 CFU/mL E. minimum limit 4.8...
Abstract Acinetobacter baumannii is a Gram-negative bacterium that has gained stronghold inside healthcare settings. Due to the ability of A. acquire antibiotic resistance easily, its presence in food products could pose major threat public health. The aim this study therefore, was investigate prevalence fresh produce and their genetic diversity. A total 234 samples vegetables fruits were collected. isolates identified using CHROMagar two different PCR assays. Also, tested for resist...
Growth media and environmental conditions influence the expression of adhesion invasion proteins in Listeria monocytogenes. Here, 104-kDa protein (LAP) was studied nutrient-rich (Trypticase soy broth [TSB] brain heart infusion [BHI]), minimal medium (Luria-Bertani [LB]), or nutrient-deficient (peptone water [PW]) by immunoblotting, enzyme-linked immunosorbent assay (ELISA), immunoelectron microscopy. Also, effect incorporating different concentrations glucose on LAP studied. Immunoblotting...