A5091591327- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- T-cell and B-cell Immunology
- Renal Transplantation Outcomes and Treatments
- Protein Degradation and Inhibitors
- Histone Deacetylase Inhibitors Research
- Complement system in diseases
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Renal Diseases and Glomerulopathies
- Immune responses and vaccinations
- Multiple Myeloma Research and Treatments
- Genomics and Chromatin Dynamics
- Connective Tissue Growth Factor Research
- Immune cells in cancer
- Chronic Kidney Disease and Diabetes
- Adenosine and Purinergic Signaling
- Hematopoietic Stem Cell Transplantation
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Phagocytosis and Immune Regulation
- Diet and metabolism studies
- CRISPR and Genetic Engineering
- Cytomegalovirus and herpesvirus research
- Biomedical Research and Pathophysiology
Instituto de Salud Carlos III
2018-2024
Instituto de Investigación Sanitaria del Principado de Asturias
2018-2024
Spanish Clinical Research Network
2015-2024
Gobierno del Principado de Asturias
2024
RELX Group (United States)
2023
Central University Hospital of Asturias
2011-2022
Instituto de Investigación Biosanitaria de Granada
2020-2021
Ludwig-Maximilians-Universität München
2016-2017
LMU Klinikum
2017
Hospital Universitario Fundación Jiménez Díaz
2013-2016
Proinflammatory cytokines contribute to renal injury, but the downstream effectors within kidney cells are not well understood. One candidate effector is Klotho, a protein expressed by that has antiaging properties; Klotho-deficient mice have an accelerated aging-like phenotype, including vascular injury and injury. Whether proinflammatory cytokines, such as TNF TNF-like weak inducer of apoptosis (TWEAK), modulate Klotho unknown. In mice, exogenous administration TWEAK decreased expression...
Primary/secondary hyperoxalurias involve nephrocalcinosis-related chronic kidney disease (CKD) leading to end-stage disease. Mechanistically, intrarenal calcium oxalate crystal deposition is thought elicit inflammation, tubular injury and atrophy, involving the NLRP3 inflammasome. Here, we found that mice deficient in ASC adaptor protein failed develop nephrocalcinosis, compromising conclusions on CKD. In contrast, hyperoxaluric wild-type developed profound nephrocalcinosis. inhibition using...
The longevity-promoting NAD + –dependent class III histone deacetylase Sirtuin 1 (SIRT1) is involved in stem cell function by controlling fate decision and/or regulating the p53-dependent expression of NANOG. We show that SIRT1 down-regulated precisely during human embryonic differentiation at both mRNA and protein levels decrease Sirt1 mediated a molecular pathway involves RNA-binding HuR arginine methyltransferase coactivator-associated (CARM1). down-regulation leads to reactivation key...
Background Human embryonic stem cells (hESCs) are an attractive resource for new therapeutic approaches that involve tissue regeneration. hESCs have exhibited low immunogenicity due to levels of Mayor Histocompatibility Complex (MHC) class-I and absence MHC class-II expression. Nevertheless, the mechanisms regulating expression in had not been explored. Methodology/Principal Findings We analyzed classical non-classical class-I, molecules, antigen-processing machinery (APM) components NKG2D...
The role of MICA antibodies in acute heart allograft rejection was examined utilizing 190 pre- and post-transplant serum samples from 44 patients collected during the first year after transplantation. were detected by CDC test on recombinant cell lines newly developed Luminex antibody detection assay. Additionally, expression analyzed 'real time' RT-PCR immunohistochemistry 10 endomyocardial biopsies. Only two subjects had HLA post-transplant. Nevertheless, found a significant number...
Global mechanisms defining the gene expression programs specific for hematopoiesis are still not fully understood. Here, we show that promoter DNA demethylation is associated with activation of hematopoietic-specific genes. Using genome-wide methylation arrays, identified 694 genes repressed by in human embryonic stem cells and whose loss hematopoietic can be expression. The association between was studied many including CD45, CD34, CD28, CD19, T cell receptor (TCR), MHC class II HLA-DR,...
Renal inflammation has a key role in the onset and progression of immune- nonimmune-mediated renal diseases. Therefore, search for novel anti-inflammatory pharmacologic targets is great interest pathology. JQ1, small molecule inhibitor bromodomain extraterminal (BET) proteins, was previously found to preserve function experimental polycystic kidney disease. We report here that JQ1-induced BET inhibition modulated vitro expression genes involved several biologic processes, including immune...
Developmental genes are silenced in embryonic stem cells by a bivalent histone-based chromatin mark. It has been proposed that this mark also confers predisposition to aberrant DNA promoter hypermethylation of tumor suppressor (TSGs) cancer. We report here silencing significant proportion these TSGs human and adult is associated with hypermethylation. Our results indicate role for methylation the control gene expression suggest that, repressed vivo, process cancer could be understood as...
The human activating receptor NKG2D is mainly expressed by NK, NKT, γδ T and CD8(+) cells and, under certain conditions, CD4(+) cells. This recognizes a diverse family of ligands (MICA, MICB ULBPs 1-6) leading to the activation effector triggering lysis target receptor-ligand system plays an important role in immune response infections, tumors, transplanted graft autoantigens. Elucidation regulatory mechanisms therefore essential for therapeutic purposes. In this study, we speculate whether...
Abstract Epigenetic mechanisms play a critical role during differentiation of T cells by contributing to the formation stable and heritable transcriptional patterns. To better understand memory maintenance in CD8+ cells, we performed genome-wide analysis DNA methylation, histone marking (acetylated lysine 9 H3 trimethylated histone), gene-expression profiles naive, effector (EM), terminally differentiated EM (TEMRA) cells. Our results indicate that demethylation acetylation are coordinated...
SARS-CoV-2 uses the angiotensin-converting enzyme 2 (ACE2) and neuropilin-1 (NRP1) receptors for entry into cells, serine protease TMPRSS2 S protein priming. Inhibition of activity or engagement with ACE2 NRP1 has been shown to be an effective strategy blocking infectivity viral spreading. Valproic acid (VPA; 2-propylpentanoic acid) is epigenetic drug approved clinical use. It produces potent antiviral anti-inflammatory effects through its function as a histone deacetylase (HDAC) inhibitor....
Increased expression of AP-1 transcription factor components has been reported in acute kidney injury (AKI). However, the role specific components, such as Fosl1, tubular cells or AKI is unknown. Upstream regulator analysis murine nephrotoxic transcriptomics identified highly upregulated. Among canonical Fosl1 was found to be upregulated two datasets from induced by folic acid cisplatin and proximal exposed TWEAK, a cytokine mediator AKI. minimally expressed kidneys control uninjured mice....
Short-chain fatty acids (SCFAs), mainly acetate, propionate and butyrate, are produced by gut microbiota through fermentation of complex carbohydrates that cannot be digested the human host. They affect health can contribute at distal level to pathophysiology several diseases, including renal pathologies.