A5047790411- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Renal Transplantation Outcomes and Treatments
- Reproductive System and Pregnancy
- Cytomegalovirus and herpesvirus research
- Organ Transplantation Techniques and Outcomes
- Diabetes and associated disorders
- Transplantation: Methods and Outcomes
- Immune Response and Inflammation
- CAR-T cell therapy research
- Streptococcal Infections and Treatments
- Influenza Virus Research Studies
- Neuroblastoma Research and Treatments
- Sarcoma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Cancer, Hypoxia, and Metabolism
- Asthma and respiratory diseases
- Microbial infections and disease research
- Pancreatic function and diabetes
- Blood groups and transfusion
- IL-33, ST2, and ILC Pathways
- Liver Disease Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- Antimicrobial Resistance in Staphylococcus
Hospital Universitario Virgen de la Arrixaca
2007-2023
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2009-2022
Animal Health Trust
2016-2020
Instituto Murciano de Investigación Biosanitaria
2019-2020
Universidad Católica San Antonio de Murcia
2020
University of Cambridge
2013-2016
Wellcome Trust
2016
Hospital Universitario Son Dureta
2008
Hospital General Universitario Gregorio Marañón
2008
Pediatric Oncology Group
2008
The role of MICA antibodies in acute heart allograft rejection was examined utilizing 190 pre- and post-transplant serum samples from 44 patients collected during the first year after transplantation. were detected by CDC test on recombinant cell lines newly developed Luminex antibody detection assay. Additionally, expression analyzed 'real time' RT-PCR immunohistochemistry 10 endomyocardial biopsies. Only two subjects had HLA post-transplant. Nevertheless, found a significant number...
Summary Tumour necrosis factor alpha ( TNF ‐α) has an important role in inflammatory response. Alterations the regulation of ‐α have been implicated a variety disorders, including Inflammatory bowel disease IBD ). Indeed, common treatment for is use inhibitors. Polymorphisms promoter region are known to affect level gene expression. Our aim was investigate influence these single nucleotide polymorphisms SNP s) play risk Spanish population and their individual response anti‐ treatment. DNA...
In Brief Background Killer immunoglobulin-like receptors (KIRs) bind human leukocyte antigen (HLA) class-I (HLA-I) ligands and regulate functions of natural killer cells subsets T cells. KIR/HLA-I interactions allow predicting cell alloreactivity in hematopoietic stem transplantation HLA-compatible kidney transplants, but its meaning liver remains controversial. Methods KIR HLA genotypes were studied 402 using sequence-specific oligonucleotides primer methods. Recipients donor KIRs, HLA-C...
Allergic bronchopulmonary aspergillosis (ABPA) is a hypersensitivity pulmonary disease that affects both patients with cystic fibrosis (CF) and those asthma. HLA-DRB1 alleles have previously been associated ABPA-CF susceptibility; however, HLA-DQB1 allele associations not clearly established. The aim of the present study was to investigate HLA class II in determine their roles susceptibility or protection. Patients ABPA-CF, CF without ABPA, asthma ABPA (AST), healthy controls were included...
Abstract Natural killer cell (NKc)-based therapies offer promising outcomes in patients with tumors, but they could improve appropriate selection of donors and optimization methods to expand NKcs vitro. Education through licensing interactions inhibitory immunoglobulin-like receptors (iKIR) NKG2A their cognate HLA class-I ligands optimizes NKc functional competence. This work has evaluated the role differentiation survival cancer patients. We have analyzed KIR KIR-ligand genes, expression...
The LILRs are a family of receptors that regulate the activities myelomonocytic cells. We found specific allelic variants two related members LILR family, LILRB3 and LILRA6, interact with ligand exposed on necrotic glandular epithelial extracellular domains LILRA6 very similar their genes highly polymorphic. A commonly occurring allele, LILRB3*12, displayed particularly strong binding these cells further screening products alleles identified motifs correlated binding. Immunoprecipitation...
Killer cell immunoglobulin-like receptors (KIRs), expressed on natural killer cells and T cells, have considerable biomedical relevance playing significant roles in immunity, pregnancy transplantation. The KIR locus is one of the most complex polymorphic regions human genome. Extensive sequence homology copy number variation makes KIRs technically laborious expensive to type. To aid investigation disease we developed a high-throughput, multiplex real-time polymerase chain reaction method...
The immune response against melanoma can be influenced by cytokines with potentially opposite effects on tumour cell growth, such as interleukin-10 (IL10), interleukin-6 (IL6) and interferon-γ (IFNγ). Our objective in this study was to investigate whether polymorphisms the regulatory regions of IL10, IL6 IFNγ genes are associated development primary cutaneous and/or prognosis tumour. We studied genotypic variations at positions −1082, −819 −592 IL10 promoter, −174 promoter +874 intron 1 42...
Leukocyte immunoglobulin-like receptors (LILR) are cell surface molecules that regulate the activities of myelomonocytic cells through balance inhibitory and activation signals. LILR genes located within leukocyte receptor complex (LRC) on chromosome 19q13.4 adjacent to KIR genes, which subject allelic copy number variation (CNV). LILRB3 (ILT5) LILRA6 (ILT8) highly polymorphic with similar extracellular domains. contains ITIM motifs is coupled an adaptor activating ITAM motifs. We analysed...
Missing self recognition makes cancer sensitive to natural killer cell (NKc) reactivity. However, this model disregards the NKc licensing effect, which highly increases reactivity through interactions of inhibitory immunoglobulin-like receptors (iKIR) with their cognate HLA-I ligands. The influence iKIR/HLA-ligand (HLA-C1/C2) on susceptibility and progression plasma (PC) dyscrasias was evaluated in 164 Caucasian patients 286 controls. Compared controls, myeloma accumulates KIR2DL1-L2+L3-...
The promyelocytic leukemia zinc finger ( PLZF ) gene, involved in rare cases of acute leukemia, encodes a Krüppel-type transcription factor. It has been reported that affects myeloid cell growth, differentiation, and apoptosis. However, the function lymphoid compartment, where is also expressed, remains largely unknown. To investigate potential relationship between expression lymphocytes programmed death, an inducible model stable clones Jurkat line was created by using tet-off system....
Fully human leukocyte antigens (HLA)-mismatched liver grafts are well accepted, but the HLA influence on acceptance or rejection is unclear and much less so impact of HLA-C, which may be conditioned by fact that HLA-C-encode molecules major ligands for killer cell immunoglobulin-like receptors (KIR).The HLA-C allele compatibility effect donor recipient genotype early graft CD8KIR T-cells recuperation were analyzed in a series 431 primary transplants. Standard polymerase chain reaction...