A5007294959- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Epigenetics and DNA Methylation
- Advanced Proteomics Techniques and Applications
- Plant Stress Responses and Tolerance
- Plant Pathogens and Fungal Diseases
- Algal biology and biofuel production
- Plant tissue culture and regeneration
- Metabolomics and Mass Spectrometry Studies
- Plant Reproductive Biology
- Forest ecology and management
- Cancer Genomics and Diagnostics
- Plant Water Relations and Carbon Dynamics
- Ferroptosis and cancer prognosis
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Seed Germination and Physiology
- Biocrusts and Microbial Ecology
- Light effects on plants
- Microbial Community Ecology and Physiology
- Plant-Microbe Interactions and Immunity
- Plant responses to elevated CO2
- Mass Spectrometry Techniques and Applications
Universidad de Oviedo
2015-2024
University of Aveiro
2014-2019
University of Vienna
2010-2016
Czech Academy of Sciences, Global Change Research Institute
2013-2016
Czech Academy of Sciences
2013-2016
University of Córdoba
2008-2010
Consejo Superior de Investigaciones Científicas
2007-2010
Instituto de Investigaciones Agrobiológicas de Galicia
2007
Instituto de Biotecnología de León
2007
Significance Ammonia-oxidizing archaea (AOA), key players in global biogeochemical cycles, represent a heterogeneous group with broad environmental distribution. Understanding their activity and physiology is of great importance due to the impact overuse agricultural fertilizers on N cycle production greenhouse gas 2 O during nitrification. Despite prominent ecological role, little known about fundamental metabolic processes AOA. Here, we show that AOA marine terrestrial environments share...
Chlamydomonas reinhardtii is one of the most important model organisms nowadays phylogenetically situated between higher plants and animals (Merchant et al. 2007). Stress adaptation this unicellular algae in focus because its relevance to biomass biofuel production. Here, we have studied cold stress C. hitherto not described for whereas intensively plants. Toward goal, high throughput mass spectrometry was employed integrate proteome, metabolome, physiological cell-morphological changes...
Aiming to mimic a more realistic field condition and determine convergent divergent responses of individual stresses in relation their combination, we explored physiological, biochemical metabolomic alterations after drought heat stress imposition (alone combined) recovery, using drought-tolerant Eucalyptus globulus clone. When plants were exposed alone, the main included reduced pre-dawn water potential (Ψpd) gas exchange. This was accompanied by increases malondialdehyde (MDA) total...
Summary Here, we describe a method for the combined metabolomic, proteomic, transcriptomic and genomic analysis from one single sample as major step multilevel data integration strategies in systems biology. While extracting proteins DNA , this protocol also allows separation of metabolites into polar lipid fractions, well RNA fractionation long small s, thus allowing broad range transcriptional studies. The isolated biomolecules are suitable with different methods that electrophoresis...
Nitrogen starvation is known to cause drastic alterations in physiology and metabolism leading the accumulation of lipid bodies many microalgae, it thus presents an important alternative for biofuel production. However, despite importance this process, molecular mechanisms that mediate metabolic remodeling induced by N especially stress recovery are still poorly understood, new candidates bioengineering needed make process useful We have studied changes involved adaptive full vegetative...
Presently, pearl millet and wheat are belonging to highly important cereal crops. Pearl millet, however, is an under-utilized crop, despite its superior resilience drought heat stress in contrast wheat. To investigate this more detail, we performed comparative physiological screening large scale proteomics of responses drought-tolerant susceptible genotypes These chosen widely used breeding farming practices. The demonstrated differences the regulation root morphology photosynthetic...
Microplastics are one of the major pollutants in aquatic environments. Among their components, Bisphenol A (BPA) is most abundant and dangerous, leading to endocrine disorders deriving even different types cancer mammals. However, despite this evidence, xenobiotic effects BPA over plantae microalgae still need be better understood at molecular level. To fill gap, we characterized physiological proteomic response Chlamydomonas reinhardtii during long-term exposure by analyzing biochemical...
Trees necessarily experience changes in temperature, requiring efficient short-term strategies that become crucial environmental change adaptability. DNA methylation and histone posttranslational modifications have been shown to play a key role both epigenetic control plant functional status under stress by controlling the state of chromatin gene expression. Cork oak (Quercus suber L.) is stone Mediterranean region, growing at temperatures 45°C. This species was subjected cumulative...
Natural variation of the metabolome Pinus pinaster was studied to improve understanding its role in adaptation process and phenotypic diversity. The metabolomes needles apical basal section buds were analysed ten provenances P. pinaster, selected from France, Spain Morocco, grown a common garden for 5 years. employment complementary mass spectrometry techniques (GC-MS LC-Orbitrap-MS) together with bioinformatics tools allowed reliable quantification 2403 molecular masses. analysis showed...
Abstract The elucidation of plant health status requires quantifying multiple molecular metabolism markers. Until now, the extraction these biomarkers is performed independently, with different extractions and protocols. This approach inefficient, since it increases laboratory time, amount sample, could introduce biases or difficulties when comparing data. To limit drawbacks, we a versatile protocol for seven most commonly analysed (photosynthetic pigments, free amino acids, soluble sugars,...
Globally expected changes in environmental conditions, especially the increase of UV irradiation, necessitate extending our knowledge mechanisms mediating tree species adaptation to this stress. This is crucial for designing new strategies maintain future forest productivity. Studies focused on environmentally realistic dosages irradiation are scarce. Pinus spp. commercially relevant trees and not much known about their UV. In work, treatment recovery radiata plants with mimicking scenarios,...
Pollen development in angiosperms is one of the most important processes controlling plant reproduction and thus productivity. At same time, pollen highly sensitive to environmental fluctuations, including temperature, drought, nutrition. Therefore, biology a major focus applied studies breeding approaches for improving productivity globally changing climate. The accessible developmental stages are mature tubes, these frequently analyzed. To reveal complete quantitative proteome map, we...