A5079642778- Genetic Mapping and Diversity in Plants and Animals
- Genetic and phenotypic traits in livestock
- Biochemical Analysis and Sensing Techniques
- RNA and protein synthesis mechanisms
- Adipose Tissue and Metabolism
- Genomic variations and chromosomal abnormalities
- Peroxisome Proliferator-Activated Receptors
- Animal Nutrition and Physiology
- Genomics and Phylogenetic Studies
- Cancer-related molecular mechanisms research
- Meat and Animal Product Quality
- Molecular Biology Techniques and Applications
- Lipid metabolism and biosynthesis
- Genetic diversity and population structure
- Wildlife Ecology and Conservation
- Chromosomal and Genetic Variations
- Genetic Associations and Epidemiology
- Microbial Metabolism and Applications
- Regulation of Appetite and Obesity
- Species Distribution and Climate Change
- Animal Ecology and Behavior Studies
- Cardiovascular Disease and Adiposity
- RNA Research and Splicing
- Animal Behavior and Welfare Studies
- Genetics and Plant Breeding
Universitat Autònoma de Barcelona
2012-2025
Center for Research in Agricultural Genomics
2012-2016
The leptin receptor gene ( LEPR ) is a candidate for traits related to growth and body composition, located on SSC6 in region where fatness meat composition quantitative trait loci (QTL) have previously been detected several F 2 experimental designs. aims of this work were: (i) fine map these QTL larger sample animals generations (F 3 backcross) an Iberian×Landrace intercross (ii) examine the effects alleles traits. Eleven single nucleotide polymorphisms (SNPs) were by sequencing coding...
Recent studies in pigs have detected copy number variants (CNVs) using the Comparative Genomic Hybridization technique arrays designed to cover specific porcine chromosomes. The goal of this study was identify CNV regions (CNVRs) swine species based on whole genome SNP genotyping chips. We used predictions from three different programs (cnvPartition, PennCNV and GADA) analyze data Porcine SNP60 BeadChip. A total 49 CNVRs were identified 55 animals an Iberian x Landrace cross (IBMAP)...
The lipid content and fatty acid (FA) profile have an important impact in human health as well the technological transformation nutritional organoleptic quality of meat. A genome-wide association study (GWAS) on 144 backcross pigs (25% Iberian × 75% Landrace) was performed for 32 traits associated with intramuscular FA composition indices metabolism. GWAS carried out using Qxpak 5.0 genotyping information obtained from Porcine SNP60K BeadChip (Illumina Inc., San Diego, CA). Signals...
Abstract Non-invasive genetic analyses enable monitoring and understanding of population dynamics without disturbing wild animals. We present a non-invasive method to identify characterize the brown bear populations Cantabrian Pyrenean (of Slovenian origin). selected an efficient 61-SNP panel genotype more than 2,000 samples from both populations. Results showed successful genotyping 1,639 samples, revealing 400 distinct individuals. Genetic diversity was similar in populations,...
Estimating breast cancer risk involves quantifying genetic and non-genetic factors. This supports health interventions communication to ensure adherence screening recommendations. study evaluated the change in estimation when incorporating density polygenic score (PRS) into baseline assessment compared efficacy of two risk-assessment delivery models. two-step included 663 healthy women with a family history which no pathogenic variants were identified. First, PRS added for all participants....
The IGF2-intron3-G3072A substitution has been recently described as the causal factor of imprinted QTL for fat deposition and muscle growth detected within porcine IGF2 region. objective this study was to investigate effect in a Large White outbred population an Iberian x Landrace F2 cross. results showed that significant effects on fatness, growth, shape traits with estimated expected direction. These agree those obtained cross, where is segregating only small family. In addition, scan...
The lack of a Near Eastern genetic signature in modern European porcine breeds indicates that, although domestic pigs from the Fertile Crescent entered Europe during Neolithic, they were completely replaced by their counterparts short window time. Whilst absence such has been convincingly demonstrated at mitochondrial level, variation autosomal genomes and Sus scrofa not compared yet. Herewith, we have explored relationships among 43 wild boar (N = 21), East 19) Korea 3), 40 Iberian 16),...
The genetic determinism of blood lipid concentrations, the main risk factor for atherosclerosis, is practically unknown in species other than human and mouse. Even model organisms, little known about how determinants traits are modulated by age-specific factors. To gain new insights into this issue, we have carried out a genome-wide association study (GWAS) cholesterol (CHOL), triglyceride (TRIG) low (LDL) high (HDL) density lipoprotein concentrations measured Duroc pigs at two time points...
Intramuscular fat (IMF) content and composition have a strong impact on the nutritional organoleptic properties of porcine meat. The goal current work was to compare patterns gene expression genetic determinism IMF traits in gluteus medius (GM) longissimus dorsi (LD) muscles.
Summary We have characterized and mapped the porcine fatty acid binding protein 5, epidermal ( FABP5 ) gene. According to linkage RH mapping, this gene is located close FABP4 4, adipocyte on swine chromosome 4. resequenced 4.7 kb of in parental population an Iberian × Landrace cross (IBMAP), identifying seven SNPs arranged two distinct haplotypes. QTL association analyses IBMAP showed that strongly associated with fat deposition. haplotype analysis revealed both (clustered mammals) are major...
Summary Long-chain acyl-CoA synthetase (ACSL) catalyses the formation of long-chain from fatty acid, ATP and CoA, activating acids for subsequent reactions. thus plays an essential role in both lipid biosynthesis acid degradation. The ACSL4 gene was evaluated as a positional candidate quantitative trait loci (QTL) located between SW2456 SW1943 on chromosome X. We have sequenced 4906 bp pig mRNA. Sequence analysis allowed us to identify 10 polymorphisms 3'-UTR region elucidate two haplotypes....
Genetic parameters such as heritability and correlations of fat traits in a Duroc population were dissected using molecular markers. The heritabilities intramuscular 2 muscles, the gluteus medius LM, back 0.54, 0.48, 0.23, respectively. genetic well estimated with standardized SNP effects, being 0.65 between ∼0.37 any trait fat. overestimated when ignoring information. Twelve chromosomes showed additive variance for compared 8 Population structure was accommodated 4 different models. number...
We identified 22 polymorphisms in the adipocyte fatty-acid binding protein (FABP4) gene, a strong positional candidate gene for FAT1 locus porcine chromosome 4. The most informative polymorphism, an insertion/deletion intron 1, together with single nucleotide polymorphism 3, was genotyped cross between Iberian and Landrace pigs. After performing QTL, marker, haplotype analyses, we showed that there were at least 2 quantitative trait genes region FABP4 tightly associated to fatness. A...
In this study, we have characterized the mitochondrial diversity of 81 swine from Uganda. Median-joining network analysis D-loop sequences these individuals and others in previous studies allowed us to determine that Ugandan pigs cluster with populations West (Europe/North Africa), Far East India. addition, partial sequencing Y-chromosome UTY locus 18 domestic revealed segregation a single HY1 lineage has cosmopolitan distribution. A Western Eastern ancestry for African had been already...
Abstract We aimed to analyse the genetic diversity of Romanian wild boars and compare it with that from other boar pig populations Europe Asia. Partial sequencing mitochondrial encoded cytochrome b ( MT-CYB ) gene 36 domestic pigs (Mangalitza, Bazna Vietnamese breeds) showed Mangalitza is fairly reduced most members these two share a common haplotype. Besides, in strong contrast animals, swine did not carry Asian variants at locus. The autosomal genotyping 18 Illumina Porcine SNP60 BeadChip...
A QTL analysis of multibreed experiments (i.e., crossed populations involving more than two founder breeds) offers clear advantages over classical two-breed crosses, among them increased power and a comprehensive coverage the total genetic variability in species. An alternative to designed crosses is reanalyze jointly several different breeds. We report multibreed, multitrait SSCX that involves five six breeds, almost 3,000 genotyped individuals using truly strategy allow for any number...
A C3469T mutation at exon 3 of the pig leptin (Lep) gene has been genotyped in diverse breeds yielding controversial results with regard to its association growth, fatness and carcass traits. similar situation reported for a HpaII restriction fragment length polymorphism (RFLP) receptor (Lepr) gene, where associations were found depending on statistical model employed. The main objective our work was investigate if plasma concentrations differ pigs different Lepr RFLP genotypes. With this...
Reproductive traits, such as prolificacy, are of great interest to the pig industry. Better understanding their genetic architecture should help increase efficiency productivity through implementation marker assisted selection (MAS) programmes. The Mucin 4 (MUC4) gene has been evaluated a candidate for prolificacy QTL described in an Iberian × Meishan (Ib Me) F2 intercross. For association analyses, two previously SNPs (DQ124298:g.243A>G and DQ124298:g.344A>G) were genotyped 347 pigs from Ib...
Summary The phylogeography of the porcine X chromosome has not been studied despite unique characteristics this chromosome. Here, we genotyped 59 single nucleotide polymorphisms ( SNP s) in 312 pigs from around world, representing 39 domestic breeds and wild boars 30 countries. Overall, widespread commercial showed highest heterozygosity values, followed by African American populations. Structuring, as inferred F ST analysis molecular variance, was consistently larger non‐pseudoautosomal...
Summary The objective of this work was to analyse the porcine Fatty acid binding protein 2, intestinal ( FABP2 ) gene as a candidate for fatty composition quantitative trait loci (QTL) previously described on chromosome 8 in an Iberian by Landrace F 2 cross (IBMAP). Re‐sequencing three and eight parental animals resulted identification single‐nucleotide polymorphisms, all them localized intron 1. polymorphism :g.412T>C, 1, two additional microsatellites were genotyped IBMAP population...
Abstract Genotyping individuals using forensic or non-invasive samples such as hair fecal increases the risk of allelic amplification failure (dropout) due to low quality and quantity DNA. One way decrease genotyping errors is increase number replicates per sample. Here, we have developed software SNP+ estimate dropout probability subsequent required obtain reliable genotype with 95%. Moreover, predicts minor allele frequency compares two competing models assuming equal allele-specific...
CDP-diacylglycerol synthase (CDS) catalyzes the conversion of phosphatidic acid to CDP-diacylglycerol, an important precursor for synthesis phosphatidylinositol, phosphatidylglycerol, and cardiolipin. We amplified sequenced 2,053 bp pig CDS1 mRNA. The structure gene was determined, being very similar that human, rat, mouse genes with respect size organization 13 exons. In addition, we identified three polymorphic positions in exons 10 11. One them, A/C1006, genotyped samples belonging...
Table S1 Set of 120 SNPs submitted online for the assay design SNPlexTM genotyping system. Please note: Blackwell Publishing is not responsible content or functionality any supplementary materials supplied by authors. The publisher supporting information Any queries (other than missing content) should be directed to corresponding author article.
Summary DGAT1 is a microsomal enzyme that catalyses the final step in triglycerides synthesis. ‐deficient mice are viable, lean, fertile and resistant to diet induced obesity. We have previously identified quantitative trait loci (QTL) on chromosome 4 affects fatty acid composition an F 2 cross between Iberian × Landrace. The human gene located 8q24.3, this region aligns porcine 4, making pig suggestive positional candidate for QTL. In study, we sequenced 1679 bp of mRNA from animals five...