A5062067515- HIV Research and Treatment
- Immune Cell Function and Interaction
- HIV/AIDS drug development and treatment
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- interferon and immune responses
- HIV/AIDS Research and Interventions
- HIV-related health complications and treatments
- COVID-19 Clinical Research Studies
- Influenza Virus Research Studies
- Mosquito-borne diseases and control
- T-cell and B-cell Immunology
- Viral Infections and Vectors
- Respiratory viral infections research
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Immune responses and vaccinations
- Monoclonal and Polyclonal Antibodies Research
- Virology and Viral Diseases
- Immune cells in cancer
- Inflammatory mediators and NSAID effects
- COVID-19 Impact on Reproduction
- vaccines and immunoinformatics approaches
- Pneumocystis jirovecii pneumonia detection and treatment
- Viral-associated cancers and disorders
Vita-Salute San Raffaele University
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2025
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2015-2025
IRCCS Ospedale San Raffaele
1999-2023
The San Raffaele Telethon Institute for Gene Therapy
1999-2023
San Raffaele University of Rome
2005-2022
Global Viral
2008-2022
Hudson Institute
2018
John Wiley & Sons (United States)
2018
Milano University Press
2014
The dependence of development and homeostasis in animals on the interaction hundreds extracellular regulatory proteins with peri- glycosaminoglycan heparan sulfate (HS) is exploited by many microbial pathogens as a means adherence invasion. Heparin, widely used anticoagulant drug, structurally similar to HS common experimental proxy. Exogenous heparin prevents infection range viruses, including S-associated coronavirus isolate HSR1. Here, we show that inhibits severe acute respiratory...
ABSTRACT Tripartite motif (TRIM) protein superfamily members are emerging as important effectors of the innate immune response against viral infections. In particular, TRIM22 was reported to exert antiviral activity RNA viruses, such hepatitis B virus (HBV), encephalomyocarditis (ECMV), and human immunodeficiency type 1 (HIV-1). We demonstrate here, for first time, that is upregulated by influenza A (IAV) infection at both mRNA levels in alveolar epithelial A549 cells. Conversely, potently...
Objective: To investigate whether the CC-chemokine monocyte chemotactic protein (MCP)-1 could play a role in pathogenesis of HIV infection central nervous system. This hypothesis was suggested by previous observations, including our finding elevated cerebrospinal fluid (CSF) levels this chemokine patients with cytomegalovirus (CMV) encephalitis. Design and methods: CSF MCP-1 were determined 37 HIV-infected neurological symptoms, compared both presence severity HIV-1 encephalitis at...
The immunosuppressive and antiinflammatory cytokine interleukin (IL) 10 selectively upregulates the expression of CC chemokine receptors CCR5, 2, 1 in human monocytes by prolonging their mRNA half-life. IL-10–stimulated display an increased number cell surface for, better chemotactic responsiveness to, relevant agonists than do control cells. In addition, are more efficiently infected HIV BaL. This effect was associated to enhancement viral entry through CCR5. These data add support emerging...
HIV-1 insertions targeting BACH2 or MLK2 are enriched and persist for decades in hematopoietic cells from patients under combination antiretroviral therapy. However, it is unclear how these provide such selective advantage to infected cell clones. Here, we show that 30/87 (34%) therapy, BACH2, STAT5B activated by triggering the formation of mRNAs contain viral sequences fused splicing their first protein-coding exon. These chimeric mRNAs, predicted express full-length proteins, T regulatory...
Abstract The dependence of the host on interaction hundreds extracellular proteins with cell surface glycosaminoglycan heparan sulphate (HS) for regulation homeostasis is exploited by many microbial pathogens as a means adherence and invasion. closely related polysaccharide heparin, widely used anticoagulant drug, which structurally similar to HS common experimental proxy, can be expected mimic properties HS. Heparin prevents infection range viruses if added exogenously, including...
During the recent severe acute respiratory (SARS) outbreak, etiologic agent was identified as a new coronavirus (CoV). We have isolated SARS-associated CoV (SARS-CoV) strain by injecting Vero cells with sputum specimen from an Italian patient affected pneumonia; traveled Vietnam to Italy in March 2003. Ultrastructural analysis of infected showed virions within cell vesicles and around membrane. The full-length viral genome sequence similar those derived Hong-Kong Hotel M isolate. By using...
Previous studies identified clones of the U937 promonocytic cell line that were either permissive or nonpermissive for human immunodeficiency virus type 1 (HIV-1) replication. These investigated further in search host restriction factors could explain their differential capacity to support HIV-1 Among known screened, tripartite motif-containing protein 22 (TRIM22) was only factor constitutively expressed and absent cells. Stable TRIM22 knockdown (KD) rescued long-terminal-repeat (LTR)-driven...
Gene therapy with ex vivo-transduced hematopoietic stem/progenitor cells may represent a valid therapeutic option for monogenic immunohematological disorders such as Wiskott-Aldrich syndrome (WAS), primary immunodeficiency associated thrombocytopenia. We evaluated the preclinical safety and efficacy of human CD34+ transduced lentiviral vectors (LV) encoding WAS protein (WASp). first set up validated transduction protocol derived from bone marrow (BM) or mobilized peripheral blood (MPB) using...
Intracellular defense proteins, also referred to as restriction factors, are capable of interfering with different steps the viral life cycle. Among these, we have shown that Tripartite motif 22 (TRIM22) suppresses basal well phorbol ester-induced HIV-1 long terminal repeat (LTR)-mediated transcription, independently its E3 ubiquitin ligase activity, nuclear factor kappa-light-chain-enhancer activated B cells (NF-kB) binding U3 region and Tat interaction TAR LTR. As transcription is driven...
Biobanks are imperative infrastructures, particularly during outbreaks, when there is an obligation to acquire and share knowledge as quick possible allow for implementation of science-based preventive, diagnostic, prognostic, therapeutic strategies.We established a COVID-19 biobank with the aim collecting high-quality well-annotated human biospecimens, in effort understand pathogenic mechanisms underlying identify targets (COVID-BioB, NCT04318366). Here we describe our experience briefly...
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic has caused a significant number of fatalities and worldwide disruption. To identify drugs to repurpose treat SARS-CoV-2 infections, we established screen measure the dimerization angiotensin-converting enzyme 2 (ACE2), primary receptor for virus. This identified fenofibric acid, active metabolite fenofibrate. Fenofibric acid also destabilized receptor-binding domain (RBD) viral spike protein inhibited RBD binding ACE2...
The CC chemokine monocyte chemotactic protein-1 (MCP-1) was markedly elevated in the cerebrospinal fluid (CSF) of human immunodeficiency virus (HIV)-infected patients with cytomegalovirus (CMV) encephalitis. MCP-l CSF levelsin CMVencephalitis weremarkedly higher than those HIV-infected or without unrelated neurologic diseases, including progressivemultifocal leukoencephalopathy, cryptococcalmeningitis, toxoplasmic encephalitis, and primary lymphoma. Interleukin-8, RANTES, macrophage...
Fusion between the viral and target cell membranes is an obligatory step for infectivity of all enveloped virus, blocking this process a clinically validated therapeutic strategy. Viral fusion driven by specialized proteins which, although specific to each act through common mechanism, formation complex two heptad repeat (HR) regions. The HR regions are initially separated in intermediate termed "prehairpin", which bridges membranes, then fold onto other form 6-helical bundle (6HB), driving...