A5014999462- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Heparin-Induced Thrombocytopenia and Thrombosis
- Platelet Disorders and Treatments
- Carbohydrate Chemistry and Synthesis
- Antiplatelet Therapy and Cardiovascular Diseases
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Blood properties and coagulation
- COVID-19 Clinical Research Studies
- Blood Coagulation and Thrombosis Mechanisms
- Hemostasis and retained surgical items
- Monoclonal and Polyclonal Antibodies Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Fibroblast Growth Factor Research
- Protease and Inhibitor Mechanisms
- Venous Thromboembolism Diagnosis and Management
- interferon and immune responses
- Phytochemical Studies and Bioactivities
- Virus-based gene therapy research
- COVID-19 Impact on Reproduction
- Polysaccharides Composition and Applications
- Marine Sponges and Natural Products
- Connective tissue disorders research
- Neonatal Respiratory Health Research
Istituto di Ricerche Chimiche e Biochimiche G. Ronzoni
2011-2024
Politecnico di Milano
2009
University of Pavia
1985-1989
Istituti di Ricovero e Cura a Carattere Scientifico
1988
The dependence of development and homeostasis in animals on the interaction hundreds extracellular regulatory proteins with peri- glycosaminoglycan heparan sulfate (HS) is exploited by many microbial pathogens as a means adherence invasion. Heparin, widely used anticoagulant drug, structurally similar to HS common experimental proxy. Exogenous heparin prevents infection range viruses, including S-associated coronavirus isolate HSR1. Here, we show that inhibits severe acute respiratory...
Abstract The dependence of the host on interaction hundreds extracellular proteins with cell surface glycosaminoglycan heparan sulphate (HS) for regulation homeostasis is exploited by many microbial pathogens as a means adherence and invasion. closely related polysaccharide heparin, widely used anticoagulant drug, which structurally similar to HS common experimental proxy, can be expected mimic properties HS. Heparin prevents infection range viruses if added exogenously, including...
The determination of molecular weight (M) and distribution (MD) heparins by a novel approach, consisting high performance size exclusion chromatography (HP-SEC) combined with triple detector array (TDA) is described. HP-SEC/TDA permits the evaluation MD polymeric samples through simultaneous action three on-line detectors, right-angle laser light scattering (RALLS), refractometer (RI), viscometer. method does not require any chromatographic column calibration, thus overcoming also difficulty...
As part of a more extensive investigation on structural features different low-molecular-weight heparins (LMWHs) that can affect their biological activities, Enoxaparin, Tinzaparin and Dalteparin were characterised with regards to the distribution chain length oligosaccharides as determined by size-exclusion (SE) chromatography, well structure defined 2D-NMR spectra (HSQC). The three LMWHs also fractionated into high affinity (HA) no (NA) pools ability bind antithrombin (AT). HA fractions...
The interaction, in aqueous solution, of the synthetic pentasaccharide AGA∗IAM (GlcN,6-SO3α1–4GlcAβ1–4GlcN,3,6-SO3α1–4IdoA,2-SO3α1–4GlcN,6-SO3αOMe; where GlcN,6-SO3 is 2-deoxy-2-sulphamino-α-d-glucopyranosyl 6-sulphate, IdoA l-iduronic acid and IdoA2-SO3 2-sulphate), which exactly reproduces structure specific binding sequence heparin heparan sulphate for antithrombin III, has been studied by NMR. In presence there were marked changes chemical shifts nuclear Overhauser effects (NOEs),...
The interaction, in aqueous solution, of the synthetic pentasaccharide AGA∗IAM (GlcN,6-SO3α1–4GlcAβ1–4GlcN,3,6-SO3α1–4IdoA,2-SO3α1–4GlcN,6-SO3αOMe; where GlcN,6-SO3 is 2-deoxy-2-sulphamino-α-d-glucopyranosyl 6-sulphate, IdoA l-iduronic acid and IdoA2-SO3 2-sulphate), which exactly reproduces structure specific binding sequence heparin heparan sulphate for antithrombin III, has been studied by NMR. In presence there were marked changes chemical shifts nuclear Overhauser effects (NOEs),...
Two years since the outbreak of novel coronavirus SARS-CoV-2 (severe acute respiratory syndrome 2) pandemic, there remain few clinically effective drugs to complement vaccines. One is anticoagulant, heparin, which in 2004 was found able inhibit invasion SARS-CoV (CoV-1) and has been employed during current pandemic prevent thromboembolic complications moderate potentially damaging inflammation. Heparin also shown experimentally attachment infection susceptible cells. At high therapeutic...
Dermatan sulphate (DS) obtained from bovine and pig mucosa skin, charge-enriched fractions of a selected DS preparation, were characterized in terms charge density, M(r) disaccharide composition chondroitin ABC lyase digests, by 13C-n.m.r. spectroscopy. Besides the major IdoA-GalNAc4SO3 sequences, all preparations contain about 10% disulphated sequences (mostly IdoA2SO3-GalNAc4SO3, with minor amounts IdoA-GalNAc4,6SO3). fragments (prepared radical-catalysed depolymerization retaining...
The evaluation of weight average molecular (Mw) and distribution represents one the most controversial aspects concerning characterization low heparins (LMWHs). As commonly used method for measurement such parameters is high performance size exclusion chromatography (HP-SEC), soundness results mainly depends on appropriate calibration chromatographic columns used. With aim meeting requirement proper Mw standards LMWHs, in present work determination (Mw Mn) by HP-SEC combined with a triple...
Abstract The dependence of the host on interaction hundreds extracellular proteins with cell surface glycosaminoglycan heparan sulphate (HS) for regulation homeostasis is exploited by many microbial pathogens as a means adherence and invasion. closely related polysaccharide heparin, widely used anticoagulant drug, which structurally similar to HS common experimental proxy, can be expected mimic properties HS. Heparin prevents infection range viruses when added exogenously, including...
Recently, certain batches of heparin have been associated with an acute, rapid onset serious side-effects indicative allergic-type reactions. These reports generated significant concern regarding the possible presence a dangerous contaminant within and highlighted need to re-assess purity criteria preparations for clinical use. Given nature array all contaminants, traditional screening tests cannot safely differentiate between contaminated uncontaminated preparations. Mono- bi-dimensional...
A young women affected by Hodgkin’s disease developed chronic autoimmune thrombocytopenic purpura. Splenectomy induced normalization of her platelet count, but hemorrhagic symptoms did not disappear. The patient’s platelets aggregate in response to collagen and ADP the IgG fraction plasma same defect normal platelets. women’s recognized glycoproteins IIb IIIa membranes. Prednisone therapy disappearance bleeding aggregation
A complex of the synthetic tetrasaccharide AGA*I M [GlcN,6‐SO 3 ‐α(1–4)‐GlcA‐β(1–4)‐GlcN,3,6‐SO ‐α(1–4)‐IdoA‐αOMe] and plasma protein antithrombin has been studied by NMR spectroscopy. 1 H 13 C chemical shifts, three‐bond proton–proton ( J H‐H ) one‐bond proton–carbon coupling constants C‐H as well transferred NOEs rotating frame Overhauser effects (ROEs) were monitored a function : ligand molar ratio temperature. Considerable changes observed at both 20 10 ratios (AGA*I antithrombin) in...
A number of low molecular weight heparin (LMWH) products are available for clinical use and although all share a similar mechanism action, they classified as distinct drugs because the different depolymerisation processes native resulting in substantial pharmacokinetic pharmacodynamics differences. While enoxaparin has been extensively investigated, little information is regarding LMWH dalteparin. The present study focused on detailed structural characterization Fragmin® by LC-MS NMR applied...
Abstract The glycosaminoglycan (GAG) class of polysaccharides are utilised by a plethora microbial pathogens as receptors for adherence and invasion. GAG heparin prevents infection range viruses when added exogenously, including the S-associated coronavirus strain HSR1 more recently we have demonstrated that can block cellular invasion SARS-CoV-2. Heparin has found widespread clinical use anticoagulant drug this molecule is routinely used proxy GAG, heparan sulphate (HS), structural analogue...