A5016518414- Ion channel regulation and function
- Muscle Physiology and Disorders
- Cardiac electrophysiology and arrhythmias
- Adipose Tissue and Metabolism
- Neuroscience and Neuropharmacology Research
- Muscle metabolism and nutrition
- Exercise and Physiological Responses
- Genetic Neurodegenerative Diseases
- Nicotinic Acetylcholine Receptors Study
- Analytical Chemistry and Chromatography
- Silk-based biomaterials and applications
- Cholinesterase and Neurodegenerative Diseases
- Cardiomyopathy and Myosin Studies
- Neuroscience and Neural Engineering
- Analytical Chemistry and Sensors
- Ion Transport and Channel Regulation
- Metabolism, Diabetes, and Cancer
- Insect and Pesticide Research
- Spaceflight effects on biology
- Growth Hormone and Insulin-like Growth Factors
- Histone Deacetylase Inhibitors Research
- Sirtuins and Resveratrol in Medicine
- Nutrition and Health in Aging
- Analytical Methods in Pharmaceuticals
- Chemical synthesis and alkaloids
University of Bari Aldo Moro
2014-2024
Instituto di Biofisica
2010
Abstract Background Cachexia is a wasting condition associated with cancer types and, at the same time, serious and dose‐limiting side effect of chemotherapy. Skeletal muscle loss one main characteristics cachexia that significantly contributes to functional impairment. Calcium‐dependent signaling pathways are believed play an important role in skeletal decline observed cachexia, but whether intracellular calcium homeostasis affected this situation remains uncertain. Growth hormone...
Progressive weakness is a typical feature of Duchenne muscular dystrophy (DMD) patients and exacerbated in the benign mdx mouse model by vivo treadmill exercise. We hypothesized different threshold for functional adaptation muscles response to duration exercise protocol. In was confirmed grip strength after 4, 8, 12 wk mice. Torque measurements revealed that exercise-related mice correlated with protocol, while wild-type (WT) were stronger. Twitch tetanic forces isolated diaphragm extensor...
Muscle fibers lacking dystrophin undergo a long-term alteration of Ca
In age-related sarcopenia, the gradual loss of skeletal muscle mass, function and strength is underpinned by an imbalanced rate protein synthesis/breakdown. Hence, adequate intake considered a valuable strategy to mitigate sarcopenia. Here, we investigated effects 12-week oral supplementation with branched-chain amino acids (BCAAs: leucine, isoleucine, valine) recognized anabolic properties, in 17-month-old (AGED) C57BL/6J male mice. BCAAs (2:1:1) were formulated drinking water, alone or...
Disuse of postural slow‐twitch muscles, as it occurs in hypogravity, induces a slow‐to‐fast myofibre type transition. Nothing is known about the effects weightlessness on resting membrane chloride conductance (gCl), which controls sarcolemma excitability and influences fibre transition during development adult life. Using current–clamp method, we observed that rat hindlimb unloading (HU) for 1–3 weeks increased gCl fibres soleus (Sol) muscle toward values found fast muscle. Northern blot...
Orphenadrine is a drug acting on multiple targets, including muscarinic, histaminic, and NMDA receptors. It used in the treatment of Parkinson's disease musculoskeletal disorders. also as an analgesic, although its mechanism action still unknown. Both physiological pharmacological results have demonstrated critical role for voltage-gated sodium channels many types chronic pain syndromes. We tested hypothesis that orphenadrine may block channels. By using patch-clamp experiments, we evaluated...
Abstract The discovery of pathogenetic mechanisms is essential to identify new therapeutic approaches in Amyotrophic Lateral Sclerosis (ALS). Here we investigated the role most important ion channels skeletal muscle an ALS animal model (MLC/SOD1 G93A ) carrying a mutated SOD1 exclusively this tissue, avoiding motor-neuron involvement. Ion are fundamental proteins for function, and also sustain neuromuscular junction nerve integrity. By multivariate statistical analysis, using machine...
Although the sodium channel blocker, mexiletine, is first choice drug in myotonia, some myotonic patients remain unsatisfied due to contraindications, lack of tolerability, or incomplete response. More therapeutic options are thus needed for patients, which require clinical trials based on solid preclinical data. In previous structure-activity relationship studies, we identified two newly-synthesized derivatives tocainide, To040 and To042, with greatly enhanced potency use-dependent behavior...
Introduction Growth hormone secretagogues (GHSs) exert multiple actions, being able to activate GHS-receptor 1a, control inflammation and metabolism, enhance GH/insulin-like growth factor-1 (IGF-1)-mediated myogenesis, inhibit angiotensin-converting enzyme. These mechanisms are of interest for potentially targeting steps pathogenic cascade in Duchenne muscular dystrophy (DMD). Methods Here, we aimed provide preclinical evidence potential benefits GHSs DMD, via a multidisciplinary vivo ex...
Pleiotrophin (PTN) is a widespread cytokine involved in bone formation, neurite outgrowth, and angiogenesis. In skeletal muscle, PTN upregulated during myogenesis, post-synaptic induction, regeneration after crushing, but little known regarding its effects on muscle function. Here, we describe the of slow-twitch soleus fast-twitch extensor digitorum longus (EDL) muscles mice over-expressing under control promoter. The were maintained normal loading or disuse condition, induced by hindlimb...
Abstract Metaglidasen is a fibrate‐like drug reported as selective modulator of peroxisome proliferator‐activated receptor γ (PPARγ), able to lower plasma glucose levels in the absence side effects typically observed with thiazolidinedione antidiabetic agents current use. Herein we report an improved synthesis metaglidasen′s metabolically active form halofenic acid ( R )‐ 2 and that its enantiomer S . The activity two stereoisomers was carefully examined on PPARα PPARγ subtypes. As expected,...
Muscle disuse produces severe atrophy and a slow-to-fast phenotype transition in the postural Soleus (Sol) muscle of rodents. Antioxidants, amino-acids growth factors were ineffective to ameliorate atrophy. Here we evaluate effects nandrolone (ND), an anabolic steroid, on mouse skeletal induced by hindlimb unloading (HU). Mice pre-treated for 2-weeks before HU during HU. weight total protein content reduced mice restoration these parameters was found ND-treated mice. The analysis gene...
Slow-twitch muscles, devoted to postural maintenance, experience atrophy and weakness during muscle disuse due bed-rest, aging or spaceflight. These conditions impair motion activities can have survival implications. Human animal studies demonstrate the anabolic role of IGF-1 on skeletal suggesting its interest as a countermeasure. Thus, we tested overexpression alteration hindlimb unloading (HU) by using MLC/mIgf-1 transgenic mice expressing under transcriptional control MLC promoter,...