A5022163912- Ion Transport and Channel Regulation
- Electrolyte and hormonal disorders
- Ion channel regulation and function
- Apelin-related biomedical research
- Magnesium in Health and Disease
- Phagocytosis and Immune Regulation
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Pancreatic function and diabetes
- Muscle Physiology and Disorders
- Liver Disease Diagnosis and Treatment
- Hormonal Regulation and Hypertension
- Nuclear Structure and Function
- Renal and related cancers
- Lipid Membrane Structure and Behavior
- Endoplasmic Reticulum Stress and Disease
- Diet, Metabolism, and Disease
- Adipose Tissue and Metabolism
- Neonatal Respiratory Health Research
- Muscle metabolism and nutrition
- Receptor Mechanisms and Signaling
- Dialysis and Renal Disease Management
- Renal function and acid-base balance
- Multiple Sclerosis Research Studies
University of Bari Aldo Moro
2016-2025
Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies
2016-2024
University of Verona
2024
Casa Sollievo della Sofferenza
2024
Weatherford College
2024
National Research Council
2016-2023
Istituto Nazionale Biostrutture e Biosistemi
2014-2023
Telethon Institute Of Genetics And Medicine
2015-2020
The University of Texas MD Anderson Cancer Center
2017
Consorzio Roma Ricerche
2015-2016
Mitochondria are remarkably plastic organelles constantly changing their shape to fulfil various functional activities. Although the osmotic movement of water into and out mitochondrion is central for its morphology activity, molecular mechanisms pathways transport across inner mitochondrial membrane (IMM), main barrier molecules moving organelle, completely unknown. Here, we show presence a member aquaporin family channels, AQP8, demonstrate strikingly high permeability (Pf) characterizing...
Abstract Neuromyelitis optica (NMO) is an inflammatory autoimmune demyelinating disease of the central nervous system (CNS) which in autoantibodies produced by patients with NMO (NMO‐IgG) recognize a glial water channel protein, Aquaporin‐4 (AQP4) expressed as two major isoforms, M1‐ and M23‐AQP4, plasma membrane form orthogonal arrays particles (OAPs). AQP4‐M23 OAP‐forming isoform, whereas AQP4‐M1 alone unable to OAPs. The function AQP4 organization into OAPs normal physiology unknown;...
Regulatory volume decrease (RVD) is a process by which cells restore their original in response to swelling. In this study, we have focused on the role played two different Aquaporins (AQPs), Aquaporin‐4 (AQP4), and Aquaporin‐1 (AQP1), triggering RVD mediating calcium signaling astrocytes under hypotonic stimulus. Using biophysical techniques measure water flux through plasma membrane of wild‐type (WT) AQP4 knockout (KO) an astrocyte cell line (DI TNC1) transfected with or AQP1, here show...
ALS is a devastating and debilitating human disease characterized by the progressive death of upper lower motor neurons. Although much effort has been made to elucidate molecular determinants underlying onset progression disorder, causes remain largely unknown. In present work, we have deeply sequenced whole transcriptome from spinal cord ventral horns post-mortem donors affected sporadic form (which comprises ~90% cases but which less investigated than inherited disease). We observe 1160...
Family-Centred Care (FCC) is recognized as an important component of all paediatric care, including neonatal although practical clinical guidelines to support this care model are still needed in Italy. The characteristics and services for families Italian NICUs show a lack organization participation. first aim was compare satisfaction stress levels two groups parents: FCC group non-FCC (NFCC). second evaluate body weight gain the newborns enrolled. This non-randomized, prospective cohort...
Lysosomal degradation of the endoplasmic reticulum (ER) via autophagy (ER-phagy) is emerging as a critical regulator cell homeostasis and function. The recent identification ER-phagy receptors has shed light on molecular mechanisms underlining this process. However, signaling pathways regulating in response to cellular needs are still largely unknown. We found that nutrient responsive transcription factors TFEB TFE3-master regulators lysosomal biogenesis autophagy-control by inducing...
We report a detailed study of AQP4 expression in the neuromuscular system mdx mice. Immunocytochemical analysis performed by double immunostaining revealed that mice manifest progressive reduction at sarcolemmal level skeletal muscle fast fibers and type IIB are first to this expression. No labeling was observed cytoplasm fibers, indicating sarcolemma staining is not associated with an intracellular compartmentalization mistargeted protein. By Western blot RT-PCR analysis, we found whereas...
In this study we analyzed the expression of aquaporin-4 (AQP4) in mammalian skeletal muscle. Immunohistochemical experiments revealed that affinity-purified AQP4 antibodies stained selectively sarcolemma fast-twitch fibers. By immunogold electron microscopy, little or no intracellular labeling was detected. Western blot analysis showed presence two immunopositive bands with apparent molecular masses 30 and 32 kD specifically present membrane fraction a rat muscle (extensor digitorum longus,...
Abstract In this study, we investigated the involvement of blood‐brain barrier (BBB) in brain dystrophin‐deficient mdx mouse, an experimental model Duchenne muscular dystrophy (DMD). To purpose, used two tight junction markers, Zonula occludens (ZO‐1) and claudin‐1 proteins, a glial marker, aquaporin‐4 (AQP4) protein, whose expression is correlated with BBB differentiation integrity. Results showed that most microvessels mice were lined by altered endothelial cells open junctions surrounded...
In this study, we have investigated the expression of aquaporin 4 during blood-brain barrier development in optic tectum chick embryos and newly hatched chicks, by means western-blot, reverse transcriptase-polymerase chain reaction, immunohistochemistry, freeze-fracture high-resolution immunogold electron microscopy. tecta day-14 embryos, western blot analysis revealed an approx. 30 kDa band, immunoreactive for aquaporin-4, which was increased day-20 chicks. Semi-quantitative transcriptase...
We have recently demonstrated that inhibition of Rho GTPase with Clostridium difficile toxin B, or botulinum C3 toxin, causes actin depolymerization and translocation aquaporin 2 (AQP2) in renal CD8 cells the absence hormonal stimulation. Here we demonstrate is part signal transduction cascade activated by vasopressin leading to AQP2 insertion into apical membrane. Quantitation active RhoA (GTP-bound) selective pull down experiments amount decreased upon stimulation cAMP-elevating agent...
Recent studies indicate a key role of aquaporin (AQP) 4 in astrocyte swelling and brain edema suggest that AQP4 inhibition may be new therapeutic way for reducing cerebral water accumulation. To understand the physiological AQP4-mediated astroglial swelling, we used 21-nucleotide small interfering RNA duplexes (siRNA) to specifically suppress expression primary cultures. Semiquantitative RT-PCR experiments Western blot analysis showed silencing determined progressive parallel reduction mRNA...
Thyroid cancer is the most common endocrine cancer, but its key oncogenic drivers remain undefined. In this study we identified TYRO3 and AXL receptor tyrosine kinases as transcriptional targets of chemokine CXCL12/SDF-1 in CXCR4-expressing thyroid cells. Both receptors were constitutively expressed cell lines not normal displayed high levels phosphorylation due to constitutive expression ligand GAS6. human carcinoma specimens, tissues, GAS6 often coexpressed. expressing both ligand,...
Lipolytic glycerol, released from adipocytes, flows through the bloodstream to liver, where its utilisation in supplying hepatocyte gluconeogenesis is rate-limited by permeation step. An aquaglyceroporin expressed hepatocytes, aquaporin-9 (AQP9), has been often linked liver uptake of glycerol. However, truthfulness this postulation and potential existence additional pathways glycerol import hepatocytes have never assessed directly. Here, we define identity extent transport evaluate...
Regulation of water homeostasis is a central feature nervous system pathophysiology. In this context, several lines evidence suggest crucial role for the channel aquaporin-4 (AQP4) and its plasma membrane supramolecular organization as key element. Here, we demonstrate expression in tissues additional isoforms AQP4 characterized by C-terminal extension generated programmed translational readthrough. These extended (AQP4ex) display perivascular polarization dystrophin-dependent pools. AQP4ex...
Neuromyelitis Optica (NMO) is an autoimmune demyelinating disease, characterized by the presence of autoantibody (NMO-IgG) to Aquaporin-4 (AQP4). NMO-IgG identification supports NMO diagnosis and several diagnostic tests have been developed, but their sensitivity too variable, some assay show low sensitivity. This impairs correct NMO. By cell based (CBA) we here evaluate efficacy different strategies express AQP4 in mammalian cells terms of: a) translation initiation signals; b) isoforms (M1...