A5059088289- Tuberous Sclerosis Complex Research
- Vascular Tumors and Angiosarcomas
- Eosinophilic Disorders and Syndromes
- Axon Guidance and Neuronal Signaling
- Nerve injury and regeneration
- Toxin Mechanisms and Immunotoxins
- Renal and related cancers
- Calcium signaling and nucleotide metabolism
- Histiocytic Disorders and Treatments
- PARP inhibition in cancer therapy
- Multiple Myeloma Research and Treatments
- Lymphatic Disorders and Treatments
- Neuropeptides and Animal Physiology
- Histone Deacetylase Inhibitors Research
- Neurogenesis and neuroplasticity mechanisms
- Cell Adhesion Molecules Research
- Neurogenetic and Muscular Disorders Research
- Extracellular vesicles in disease
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Medical Imaging and Pathology Studies
- Lymphatic System and Diseases
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Protein Degradation and Inhibitors
- Erythrocyte Function and Pathophysiology
- Neuroscience and Neuropharmacology Research
University of Milan
2015-2024
Ospedale San Paolo
2007-2018
National Institutes of Health
1996-2001
National Heart Lung and Blood Institute
1996-2001
Pulmonary and Critical Care Associates
1998
Abstract In females, X chromosome inactivation (XCI) begins with the expression of XIST gene from destined to be inactivated (Xi) and coating RNA in cis. It has recently been reported that this process is supported by product BRCA1 tumor suppressor BRCA1−/− cancers show Xi chromatin structure defects, thus suggesting a role XCI perturbation BRCA1-mediated tumorigenesis. Using combined genetic epigenetic approach, we verified occurrence BRCA1wt breast cancer cell lines. was ascertained lost...
Multiple myeloma (MM) is an incurable hematologic neoplasm, whose poor prognosis deeply affected by the propensity of tumor cells to localize in bone marrow (BM) and induce protumorigenic activity normal BM cells, leading events associated with progression, including angiogenesis, osteoclastogenesis, spread osteolytic lesions. The interplay between MM niche does not only rely on direct cell-cell interaction, but a crucial role also played MM-derived extracellular vesicles (MM-EV). Here, we...
Our world is made of plastic. Plastic waste deeply affects our health entering the food chain. The degradation and/or fragmentation plastics due to weathering processes result in generation nanoplastics (NPs). Only a few studies tested NPs effects on human health. toxic actions are, part, mediated by oxidative stress (OS) that, among its effects, bone remodeling. This study aimed assess if influence skeleton remodeling through OS. Murine cell cultures (MC3T3-E1 preosteoblasts, MLOY-4...
Spinal cord injury (SCI) triggers a complex ischemic and inflammatory reaction, involving activation of neurotransmitter systems, in particular glutamate, culminating cell death. We hypothesized that SCI might lead to alteration the RNA editing alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors govern critical determinants neuronal survival. To this end, we examined molecular changes set motion by affect channel properties AMPA receptors. strongly reduced level receptor...
Multiple myeloma is still incurable due to an intrinsic aggressiveness or, more frequently, the interactions of malignant plasma cells with bone marrow (BM) microenvironment. Myeloma educate BM support neoplastic cell growth, survival, acquisition drug resistance resulting in disease relapse. microenvironment characterized by Notch signaling hyperactivation increased expression Notch1 and 2 ligands Jagged1 tumor cells. activation influences biology promotes reprogramming stromal In this work...
The advent of pharmacological therapies for lymphangioleiomyomatosis (LAM) has made early diagnosis important in women with tuberous sclerosis complex (TSC), although the lifelong cumulative radiation exposure caused by chest computer tomography (CT) should not be underestimated. We retrospectively investigated, a cohort TSC outpatients San Paolo Hospital (Milan, Italy) 1) role pulmonary function tests (PFTs) LAM diagnosis, 2) association between and other features (e.g. demography,...
Background Tuberous sclerosis complex (TSC), a tumor syndrome caused by mutations in TSC1 or TSC2 genes, is characterized the development of hamartomas. We previously isolated, from an angiomyolipoma patient, homogenous population smooth muscle-like cells (TSC2−/− ASM cells) that have mutation gene as well loss heterozygosity (LOH) and consequently, do not produce product, tuberin. TSC2−/− cell proliferation EGF-dependent. Methods Findings Effects EGF on transfected with were determined. In...
Abstract Tuberous sclerosis complex ( TSC ) is caused by mutations in 1 or 2 genes. Lymphangioleiomyomatosis LAM can be sporadic associated with and characterized widespread pulmonary proliferation of abnormal α‐smooth muscle ASM )‐like cells. We investigated the features cells isolated from chylous thorax a patient affected , named / cells, bearing germline mutation an epigenetic defect causing absence tuberin. Proliferation epidermal growth factor EGF )‐dependent blockade receptor causes...
Mesenchymal stem cells (MSCs) are multipotent able to differentiate into multiple cell types, including adipocytes, osteoblasts, and chondrocytes. The role of adipose-derived (ADSCs) in cancers is significantly relevant. They seem be involved the promotion tumour development progression relapse processes. For this reason, investigating effects breast cancer microenvironment on ADSCs high importance order understand relationship between surrounding stromal cells. With current study, we aimed...
Abstract This study shows that treatment of rats with exogenous glycosaminoglycans stimulates peripheral nerve regeneration, increases the abundance mRNAs for myelin proteins and promotes muscle reinnervation. After sciatic had been crushed number regenerating axons in distal stump was markedly highly significantly increased by glycosaminoglycan throughout experimental period. The correlated axon fibre (axon + myelin) diameter. P o protein basic nerves also affected glycosaminoglycans....
This study has shown that glycosaminoglycans added to the culture medium may affect neurite formation in SH-SY5Y neuroblastoma cells. The most effective are heparin and COS 8, a preparation with low anticoagulant activity. Promotion of neuritogenesis was remarkable at concentrations as 10-8 10-10. When 10-4 M both agents inhibitory. Chondroitin-4 sulfate, dermatan heparan sulfate were also effective, doses required were, however, high for promoting inhibiting neuritogenesis. Thereby promote,...
EGFR belongs to the HER/ErbB family of tyrosine kinase receptors and its activation in cancer cells has been linked with increased proliferation, angiogenesis, metastasis. Lymphangioleiomyomatosis (LAM) is a rare, low-grade neoplasm that occurs sporadically or association tuberous sclerosis complex (TSC), genetic, multisystem disorder characterized by hamartomas several organs. From chylous LAM/TSC patient, we previously isolated smooth muscle-like whose proliferation depends on EGF...
Tuberous Sclerosis Complex (TSC) is characterized by the presence of benign tumors in brain, kidneys, heart, eyes, lungs, and skin. The typical brain lesions are cortical tubers, subependimal nodules subependymal giant‐cell astrocytomas. occurrence malignant astrocytomas such as glioblastoma rare. We report on a child with clinical diagnosis TSC rapidly evolving multiforme. Genetic analysis identified de novo mutation TSC2 . Molecular characterization tumor was performed discussed, well...
Tuberous Sclerosis Complex (TSC) is a tumor suppressor gene disorder with mutations of TSC1/TSC2 genes. This leads to the development hamartomas that most frequently affect central nervous system, kidney, and skin. Angiomyolipomas are abdominal masses made up muscle vessels adipose tissues grow mostly in proximity kidneys liver. Bleeding kidney failure major justification for surgery. study shows angiomyolipoma-derived human smooth TSC2−/− cells express apoptosis inhibitor protein survivin...