A5045463860- Neuroscience and Neuropharmacology Research
- Stress Responses and Cortisol
- Renal and related cancers
- Bipolar Disorder and Treatment
- Tryptophan and brain disorders
- Pluripotent Stem Cells Research
- Birth, Development, and Health
- MicroRNA in disease regulation
- Adipose Tissue and Metabolism
- Hereditary Neurological Disorders
- RNA Research and Splicing
- Tissue Engineering and Regenerative Medicine
- Fatty Acid Research and Health
- RNA regulation and disease
- Mesenchymal stem cell research
- Genetic Neurodegenerative Diseases
- Renal cell carcinoma treatment
- Genetic and Kidney Cyst Diseases
- Diet and metabolism studies
- Botulinum Toxin and Related Neurological Disorders
- Cardiac electrophysiology and arrhythmias
- Adipokines, Inflammation, and Metabolic Diseases
- Neuroscience and Neural Engineering
- Regulation of Appetite and Obesity
- Anesthesia and Neurotoxicity Research
Hospital Italiano La Plata
2024
Universidad de la República
2024
Centro San Giovanni di Dio Fatebenefratelli
2018-2022
Istituti di Ricovero e Cura a Carattere Scientifico
2018-2022
University of Castilla-La Mancha
2017-2020
University of Brescia
2010-2019
Instituto Murciano de Investigación Biosanitaria
2018
New York University
2015-2016
Dysautonomia Foundation
2015
Columbia University Irving Medical Center
2015
The antiparkinsonian ropinirole and pramipexole are D3 receptor- (D3R-) preferring dopaminergic (DA) agonists used as adjunctive therapeutics for the treatment resistant depression (TRD). While exact antidepressant mechanism of action remains uncertain, a role D3R in restoration impaired neuroplasticity occurring TRD has been proposed. Since highly expressed on DA neurons humans, we studied effect structural plasticity using translational model human-inducible pluripotent stem cells...
Abstract Aims Atrial fibrillation (AF) is the most common type of cardiac arrhythmias, whose incidence likely to increase with aging population. It considered a progressive condition, frequently observed as complication other cardiovascular disorders. However, recent genetic studies revealed presence several mutations and variants linked AF, findings that define AF multifactorial disease. Due complex genetics paucity models, molecular mechanisms underlying initiation are still poorly...
1. The behavior of albino rats before and after injections opiates, was studied in the circular maze. 2. It found that morphin produced a depressant effect on animal, both large small doses, but case one animal drug exhibited stimulating effect. 3. Of other opium alkaloids, with exception papaverin, all were to be slightly depressant. 4. Combinations alkaloids are, whole, somewhat less than equivalent doses when given alone. 5. In experiments, even where narcotics administered, marked...
In this study we have shown that the papilla of mouse kidney contains a population Pax2+ cells are detectable from early postnatal period through to adulthood. Lineage analysis suggests some these derived metanephric mesenchyme, progenitor gives rise nephrons during organogenesis. Here describe method for isolating and culturing population, demonstrate within multipotent stem cells, as they clonogenic appear undergo unlimited self-renewal. Further, under appropriate culture conditions, can...
Clinical studies on patients with stress-related neuropsychiatric disorders reported functional and morphological changes in brain areas where glutamatergic transmission is predominant, including frontal prefrontal areas. In line this evidence, several preclinical works suggest that glutamate receptors are targets of both rapid long-lasting effects stress. Here we found acute footshock- (FS-) stress, although inducing no transcriptional RNA editing alterations ionotropic AMPA NMDA receptor...
Ionotropic glutamate α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors are the major mediators of fast synaptic neurotransmission. In this work, we used primary cortical cultures from rats as a model system to study AMPA receptor regulation during in vitro cell maturation and after activity modifications. The levels mRNA protein, along with alternative splicing RNA editing subunit (GluR1-4) mRNAs, were analyzed immature (DIV5) mature (DIV26) rat neuronal cultures. We...
Spinal cord injury (SCI) triggers a complex ischemic and inflammatory reaction, involving activation of neurotransmitter systems, in particular glutamate, culminating cell death. We hypothesized that SCI might lead to alteration the RNA editing alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors govern critical determinants neuronal survival. To this end, we examined molecular changes set motion by affect channel properties AMPA receptors. strongly reduced level receptor...
The role of central leptin in regulating the heart from lipid accumulation lean leptin-sensitive animals has not been fully elucidated. Herein, we investigated effects infusion on expression genes involved cardiac metabolism and its control myocardial triacylglyceride (TAG) adult Wistar rats. Intracerebroventricular (icv) (0.2 µg/day) for 7 days markedly decreased TAG levels tissue. Remarkably, anti-steatotic were associated with selective upregulation gene protein peroxisome...
We have recently shown that kidney-derived stem cells (KSCs) isolated from the mouse newborn kidney differentiate into a range of kidney-specific cell types. However, functionality and integration capacity these KSCs remain unknown. Therefore, main objectives this study were (1) to determine if proximal tubule-like cells, generated in vitro KSCs, displayed absorptive function typical tubule vivo, (2) establish whether ability integrate developing nephrons was comparable with metanephric...
Kainic acid (KA) binds to the AMPA/KA receptors and induces seizures that result in inflammation, oxidative damage neuronal death. We previously showed cyclooxygenase-2 deficient (COX-2−/−) mice are more vulnerable KA-induced excitotoxicity. Here, we investigated whether increased susceptibility of COX-2−/− KA is associated with altered mRNA expression editing glutamate receptors. The AMPA GluR2, GluR3 GluR6 was vehicle-injected compared wild type (WT) hippocampus cortex, whereas gene NMDA...
Hereditary sensory and autonomic neuropathy type III (HSAN III) features disturbed proprioception a marked ataxic gait. We recently showed that joint angle matching error at the knee is positively correlated with degree of ataxia. Using intraneural microelectrodes, we also documented these patients lack functional muscle spindle afferents but have preserved large-diameter cutaneous afferents, suggesting better may be relying more on proprioceptive cues provided by tactile afferents. tested...
Familial dysautonomia is an inherited autonomic disorder with afferent baroreflex failure. We questioned why despite low blood pressure standing, surprisingly few familial patients complain of symptomatic hypotension or have syncope. Using transcranial Doppler ultrasonography the middle cerebral artery, we measured flow velocity (mean, peak systolic, and diastolic), area under curve, pulsatility index, height dictrotic notch in 25 15 controls. In patients, changing from sitting to a standing...
MicroRNAs (miRNAs), one of the major small non-coding RNA classes, have been proposed as regulatory molecules in neurodevelopment and stress response. Although alterations miRNAs profiles implicated several psychiatric neurodevelopmental disorders, contribution individual brain development function is still unknown. Recent studies identified miR-19 a key regulator trajectories, since it drives differentiation neural stem cells into mature neurons. However, no findings are available on how...
Abstract Background In recent years, the identification of peripheral biomarkers that are associated with psychiatric diseases, such as Major Depressive Disorder (MDD), has become relevant because these may improve efficiency differential diagnosis process and indicate targets for new antidepressant drugs. Two candidate genes, ErbB3 Fgfr1 , growth factors whose mRNA levels have been found to be altered in leukocytes patients affected by bipolar disorder a depressive state. On this basis, aim...
Objectives: Insulin-like growth factor binding protein 2 (IGFBP2) is a member of the family high-affinity proteins (IGFBP1–6) and appears to play governing role in insulin-like (IGF) regulation central nervous system. This study aimed investigate putative involvement IGFBP2 mood disorder pathogenesis by measuring its expression levels patient peripheral tissues.Methods: mRNA were measured serum 93 controls, 41 bipolar (BD) 43 major depressive (MDD) patients skin fibroblasts from 15 12 BD 23...
Objective: The amniotic membrane (AM) is a tissue with low immunogenity and high therapeutic potential due to its anti-inflammatory, anti-fibrotic antimicrobial effects. This paper describes the use of cryopreserved allografts treat diabetic foot ulcers (DFUs) in patients diabetes. Method: In this case series, AM was processed obtain final medicinal product: membrane. applied every 7–10 days until total epithelialisation DFUs. Results: A 14 DFUs (median size: 12.30cm, (range: 0.52–42.5cm 2 )...
Abstract Background Bipolar Disorder (BD) is a complex mental disease characterized by recurrent episodes of mania and depression. Lithium (Li) represents the mainstay BD pharmacotherapy, despite narrow therapeutic index high variability in treatment response. However, although several studies have been conducted, molecular mechanisms underlying Li effects remain unclear. Methods In order to identify signatures biological pathways associated with response, we conducted transcriptome miRNome...