A5035648726- Renal Transplantation Outcomes and Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Organ Transplantation Techniques and Outcomes
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cancer, Hypoxia, and Metabolism
- Microbial infections and disease research
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- MicroRNA in disease regulation
- Bacteriophages and microbial interactions
- Liver Disease and Transplantation
- Cancer Research and Treatments
- Amino Acid Enzymes and Metabolism
- Neurological Complications and Syndromes
- Pancreatic and Hepatic Oncology Research
- Plant Virus Research Studies
- Telomeres, Telomerase, and Senescence
- Hedgehog Signaling Pathway Studies
- Orthopaedic implants and arthroplasty
- Genomics and Chromatin Dynamics
- Bacterial Genetics and Biotechnology
- Adenosine and Purinergic Signaling
- Hematopoietic Stem Cell Transplantation
- Bone and Joint Diseases
Instituto Murciano de Investigación Biosanitaria
2016-2025
Hospital Universitario Virgen de la Arrixaca
2008-2020
Universidad de Murcia
2016-2019
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2008-2017
Estación Experimental de Aula Dei
2006
Universidad de León
1998-2005
Consejo Superior de Investigaciones Científicas
2005
Background. Human liver allografts do sometimes survive in a recipient after withdrawal of immunosuppression (IS), commonly referred to as “operational tolerance.” Preliminary clinical data have suggested an increase the frequency regulatory T cells (Treg) CD4+CD25high and FoxP3 expression operationally tolerant transplant recipients (Gr-T). In context human transplantation, dynamics Treg not been studied. We designed prospective study ascertain profile population during IS withdrawal....
Immune cells are equipped with a number of receptors that recognize sterile injury and pathogens. We find host immune release ATP as an inflammatory signal in response to allogeneic transplantation. then acts via feedback mechanism on the P2X7 channel activate NLRP3 inflammasome subsequently process interleukin (IL)-18. This is necessary stage deleterious Th1 against allotransplantation interferon-γ production. Lack IL-18 resulted decrease graft-infiltrating CD8 but increase regulatory T...
The maintenance of T-cell homeostasis must be tightly regulated. Here, we have identified a coordinated role Poly(ADP-ribose) polymerase-1 (PARP-1) and PARP-2 in maintaining T-lymphocyte number function. Mice bearing specific deficiency PARP-1-deficient background showed defective thymocyte maturation diminished numbers peripheral CD4+ CD8+ T-cells. Meanwhile, was not affected single PARP-1 or PARP-2-deficient mice. lymphopenia associated with dampened vivo immune responses to synthetic...
The aim of this study is to identify a set microRNAs (miRNAs) as prognostic molecular biomarkers for the progression Barrett esophagus (BE) esophageal adenocarcinoma (EAC) rationalize surveillance programs in patients with BE.Histological dysplasia currently used main biomarker BE at high risk developing EAC. Although miRNA expression profiles and EAC have been reported, it has not established which miRNAs could constitute robust diagnostic test predict EAC.miRNAs associated were identified...
Introduction. In medullary thyroid carcinoma (MTC), calcitonin levels and TNM staging are established prognostic factors. However, the diagnostic accuracy of cytology in MTC is poor. this context, microRNAs (miRNAs) may be useful as valuable biomarkers, although evidence remains scarce. The aim study was to determine expression several miRNAs patients with investigate their association clinical aggressiveness. Methods. Twenty-five undergoing surgery for were included. miRNA profiles...
After liver transplantation, long-term immunosuppression (IS) administration is commonly complicated by renal dysfunction and cardiovascular complications. Twenty transplant patients on cyclosporine (CyA)-based IS were followed up prospectively after withdrawal. They consisted of 10 electively weaned either forcibly or incidentally patients. Liver biochemical tests, blood pressure, serum creatinine, urea, uric acid, triglycerides, cholesterol glucose monitored the start weaning. Eight 20...
Associated liver partition and portal vein ligation for staged hepatectomy (ALPPS) is a two-stage technique which can be associated with hypertrophic stimulus on the future remnant (FLR) stronger than other techniques–such as (PVL). However, reason of such hypertrophy still unclear, but it suggested that transection combined during first stage this may play key role. The aim study to compare FLR clinical changes both ALPPS PVL in rat surgical model. For purpose, three groups SD rats were...
Medication-related osteonecrosis of the jaw (MRONJ) is defined as exposed necrotic bone involving maxillofacial structures in bisphosphonate treated patients, and pathophysiology this disease remains unclear. The aim study was to assess effects allogeneic transplantation marrow-derived mesenchymal stem cells (BM-MSCs) a model Wistar mice with induced MRONJ disease. BM-MSCs from five male rats were characterized cultured on β-tricalcium phosphate (β-TCP) granules. Thirty female injected...
Regulatory T cells (Tregs) play a potential role in operational tolerance liver transplantation (LT) patients, and microRNAs (miRNAs) are known to be involved immunological responses tolerance. Thus, we analyzed the implication of different peripheral blood Treg subsets miRNAs on LT 24 tolerant (Tol) 23 non-tolerant (non-Tol) recipients by cellular, genetic, epigenetic approximation. Non-Tol patients had lower demethylation rate forkhead box P3 (FOXP3) regulatory cell-specific demethylated...
The artificial induction of tolerance in transplantation is gaining strength. In mice, a differential role extracellular adenosine (eADO) for regulatory and effector T cells (Tregs Teffs, respectively) has been proposed: inhibiting Teffs inducing Tregs. aim this study was to analyze the action nucleotides human and, moreover, examine influence CD39 CD73 ectonucleotidases subsequent signaling through 2 receptor (A2R) clinical after liver transplant. analyzed by vitro experiments with isolated...
Numerous studies have emphasized the genetic and phenotypic profiles of tolerant transplant patients. Moreover, different groups defined several biomarkers, trying to distinguish patients who are going be from those reject. However, most these biomarkers not been validated by other or even established for clinical practice.We reanalyzed stratified predictive capacity 20 previously described liver transplantation tolerance in a cohort 17 subjected an independent, nonrandomized, prospective...
N ‐Acetyl‐ D ‐mannosamine (ManNAc) and ‐acetyl‐ ‐glucosamine (GlcNAc) are the essential precursors of ‐acetylneuraminic acid (NeuAc), specific monomer polysialic (PA), a bacterial pathogenic determinant. Escherichia coli K1 uses both amino sugars as carbon sources uptake takes place through mannose phosphotransferase system transporter, phosphoenolpyruvate‐dependent that shows broad range specificity. Glucose, mannose, fructose, glucosamine strongly inhibited transport these amino‐acetylated...
Associated liver partition and portal vein ligation for staged hepatectomy (ALPPS) has been suggested as a potential therapy extensive bilobar tumors, although in some circumstances this technique may induce tumor progression, fact still not well studied. Our aim was to study hepatic progression induced by the first step of ALPPS WAG/Rij rat syngenic model metastatic colorectal carcinoma subcapsular CC531 cell line inoculation. induced: on deportalized lobe metastases; expression...
The capsular polysaccharide of Pasteurella haemolytica A2 consists a linear polymer N ‐acetylneuraminic acid (Neu5Ac) with α(2–8) linkages. production this is strictly regulated by the growth temperature and above 40°C no detected. Analysis enzymatic activities directly involved in its biosynthesis reveals that Neu5Ac lyase, CMP‐Neu5Ac synthetase polysialyltransferase are regulation. Very low were found P. grown at 43°C (at least 25 times lower than those observed when was 37°C). synthesis...
N ‐Acetyl‐ d ‐mannosamine (ManNAc) is a specific substrate for the synthesis of ‐acetylneuraminic acid, essential precursor bacterial capsular polysialic acid (PA). When Escherichia coli K92 used ManNAc as carbon source, we observed dramatic reduction (up to 90%) in vivo PA production. Experiments which source was changed revealed that maximal inhibitory effect occurred when this sugar present medium before logarithmic phase growth had started. Enzymatic analysis high concentrations...