A5073784461- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Bacterial biofilms and quorum sensing
- Aquaculture disease management and microbiota
- Advanced biosensing and bioanalysis techniques
- Vibrio bacteria research studies
- Escherichia coli research studies
- Enzyme Structure and Function
- Antibiotic Resistance in Bacteria
- DNA Repair Mechanisms
- RNA Research and Splicing
- Biochemical and Molecular Research
- Viral gastroenteritis research and epidemiology
- Biopolymer Synthesis and Applications
- CRISPR and Genetic Engineering
- Studies on Chitinases and Chitosanases
- Microbial infections and disease research
- Trypanosoma species research and implications
- Synthesis and biological activity
- Zebrafish Biomedical Research Applications
- Click Chemistry and Applications
- Microbial Community Ecology and Physiology
- Protein Structure and Dynamics
University of Milan
2016-2025
University of Verona
2023-2025
Bridge University
2024
Weatherford College
2024
University of Alabama at Birmingham
2024
Azienda Ospedaliera Universitaria Integrata Verona
2023
Centro Nacional de Biotecnología
2011
ABSTRACT The alarming diffusion of multidrug-resistant (MDR) bacterial strains requires investigations on nonantibiotic therapies. Among such therapies, the use bacteriophages (phages) as antimicrobial agents, namely, phage therapy, is a promising treatment strategy supported by findings recent successful compassionate treatments in Europe and United States. In this work, we combined host range genomic information to design 6-phage cocktail killing several clinical Pseudomonas aeruginosa ,...
Abstract Cystic fibrosis (CF) is a hereditary disease due to mutations in the CFTR gene and causes mortality humans mainly respiratory infections caused by Pseudomonas aeruginosa . In previous work we used phage therapy, which treatment with mix of phages, actively counteract acute P mice Galleria mellonella larvae. this apply therapy PAO1 CF zebrafish model. The structure channel evolutionary conserved between fish mammals cftr -loss-of-function embryos show phenotype that recapitulates...
Abstract E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated aeruginosa . Here, we describe the structure of whole virion before and after DNA ejection at 3.1 Å 4.5 resolution, respectively, determined using cryogenic electron microscopy (cryo-EM). We identify build de novo structures for 19 unique gene products, resolve tail genome-ejection machine both extended contracted states, decipher complete architecture baseplate formed by 66...
S1 is an 'atypical' ribosomal protein weakly associated with the 30S subunit that has been implicated in translation, transcription and control of RNA stability. thought to participate translation initiation complex formation by assisting positioning region, but little known about its role other transactions. In this work, we have analysed vivo effects different intracellular concentrations, from depletion overexpression, on decay distribution leadered leaderless messenger RNAs (mRNAs). We...
Elevated low-density lipoprotein cholesterol (LDL-C) levels are associated with atherosclerotic cardiovascular diseases. Inclisiran, a small interfering RNA, has been observed to effectively and sustainably reduce LDL-C in large randomized controlled trials (RCTs); however, real-world data on its short-term efficacy use limited. This study aims assess the safety of inclisiran real-life population within one month from first administration. observational, single-center, retrospective cohort...
Polynucleotide phosphorylase (PNPase, polyribonucleotide nucleotidyltransferase, EC 2.7.7.8) is one of the cold shock‐induced proteins in Escherichia coli and pnp , gene encoding it, essential for growth at low temperatures. We have analysed expression upon shock found a dramatic transient variation transcription profile: within first hour after temperature downshift amount transcripts detectable by Northern blotting increased more than 10‐fold new mRNA species that cover downstream region,...
Pseudomonas aeruginosa is a highly adaptable bacterium that thrives in broad range of ecological niches and can infect multiple hosts as diverse plants, nematodes mammals. In humans, it an important opportunistic pathogen. This wide adaptability correlates with its genetic diversity. this study, we used deep-sequencing approach to explore the complement small RNAs (sRNAs) P. number such regulatory molecules previously identified organism relatively low, considering genome size, phenotypic...
Sperm surface beta-N-acetylhexosaminidases are among the molecules mediating early gamete interactions in invertebrates and vertebrates, including man. The plasma membrane of Drosophila spermatozoa contains two beta-N-acetylhexosaminidases, DmHEXA DmHEXB, which required for egg fertilization. Here, we demonstrate that three putative melanogaster genes predicted to code Hexo1, Hexo2, fdl, all expressed male germ line. fdl codes a homolog alpha-subunit mammalian lysosomal...
Bacillus subtilis pnpA gene product, polynucleotide phosphorylase (PNPase), is involved in double-strand break (DSB) repair via homologous recombination (HR) or non-homologous end-joining (NHEJ). RecN among the first responders to localize at DNA DSBs, with PNPase facilitating formation of a discrete focus per nucleoid. PNPase, which co-purifies RecA and RecN, was able degrade single-stranded (ss) 3′ → 5′ polarity presence Mn2+ low inorganic phosphate (Pi) concentration, extend 3′-OH end...
The exoribonuclease polynucleotide phosphorylase (PNPase, encoded by pnp ) is a major player in bacterial RNA decay. In Escherichia coli , PNPase expression post-transcriptionally regulated at the level of mRNA stability. primary transcript very efficiently processed endonuclease RNase III specific site and rapidly degraded PNPase-dependent manner. While investigating autoregulation mechanism we found, UV-cross-linking experiments, that ribosomal protein S1 crude extracts binds to -mRNA...
The Escherichia coli polynucleotide phosphorylase (PNPase; encoded by pnp), a phosphorolytic exoribonuclease, posttranscriptionally regulates its own expression at the level of mRNA stability and translation. Its primary transcript is very efficiently processed RNase III, an endonuclease that makes staggered double-strand cleavage about in middle long stem-loop 5'-untranslated region. pnp then rapidly degraded PNPase-dependent manner. Two non-mutually exclusive models have been proposed to...
Transition from planktonic cells to biofilm is mediated by production of adhesion factors, such as extracellular polysaccharides (EPS), and modulated complex regulatory networks that, in addition controlling redirect bacterial cell metabolism the mode.Deletion pnp gene, encoding polynucleotide phosphorylase, an RNA processing enzyme a component degradosome, results increased formation Escherichia coli. This effect particularly pronounced E. coli strain C-1a, which deletion gene leads strong...
Modulation of mRNA translatability either by trans -acting factors (proteins or sRNAs) in cis riboregulators is widespread bacteria and controls relevant phenotypic traits. Unfortunately, global identification post-transcriptionally regulated genes complicated poor structural functional conservation regulatory elements the limitations proteomic approaches protein quantification. We devised a genetic system for we applied this to search Pseudomonas aeruginosa RNA thermometers, class that...
Pseudomonas spp. are endowed with a complex pathway for glucose uptake that relies on multiple transporters. In this work we report the construction and characterization of aeruginosa single mutants unmarked deletions genes encoding outer membrane (OM) inner (IM) proteins involved in uptake. We found triple ΔgltKGF ΔgntP ΔkguT mutant lacking all known IM transporters (named GUN Glucose Uptake Null) is unable to grow as unique carbon source. More than 500 controlling both metabolic functions...
Multi drug resistant (MDR) bacteria are insensitive to the most common antibiotics currently in use. The spread of antibiotic-resistant bacteria, if not contained, will represent main cause death for humanity 2050. situation is even more worrying when considering patients with chronic bacterial infections, such as those Cystic Fibrosis (CF). development alternative approaches essential and novel therapies that combine exogenous host-mediated antimicrobial action promising. In this work, we...