A5082348781- RNA and protein synthesis mechanisms
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Microbial infections and disease research
- DNA and Nucleic Acid Chemistry
- RNA modifications and cancer
- Biochemical and Structural Characterization
- Inhalation and Respiratory Drug Delivery
- Pneumonia and Respiratory Infections
- Aquaculture disease management and microbiota
- Viral gastroenteritis research and epidemiology
- Vibrio bacteria research studies
- Urban Stormwater Management Solutions
- Bacterial Infections and Vaccines
- Yersinia bacterium, plague, ectoparasites research
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Urban Heat Island Mitigation
- Salmonella and Campylobacter epidemiology
- Cystic Fibrosis Research Advances
- Flood Risk Assessment and Management
Karolinska Institutet
2018-2025
Karolinska University Hospital
2020-2022
Ruhr University Bochum
2013-2020
Technical University of Denmark
2017
Cowi (Norway)
2017
University of Milan
2012
Significance The RNA structure is critical for function in all domains of life. We determined the transcriptome-wide structurome Yersinia pseudotuberculosis , a food-borne pathogen, at three physiologically relevant temperatures. Our analysis shows that mRNAs tend to have poorly structured ribosome binding site. Transcripts deviate from this general principle are very good candidates as translational repressor elements, and we identified 16 thermometers able control gene expression...
Abstract Precise secondary and tertiary structure formation is critically important for the cellular functionality of ribonucleic acids (RNAs). RNA folding studies were mainly conducted in vitro, without possibility validating these experiments inside cells. Here, we directly resolve stability a hairpin‐structured live mammalian We find that cell comparable to dilute physiological buffer. On contrary, addition vitro artificial crowding agents, with exception high‐molecular‐weight PEG, leads...
The initiation, progression and transmission of most bacterial infections is dependent upon the ability invading pathogen to acquire iron from each varied environments encountered during course a natural infection. In total, 95% within human body complexed heme, making heme potentially rich source host-associated nutrient for bacteria. As only host, pathogenic bacteria often regulate synthesis utilization factors such that production maximal under environmental conditions. This study...
Pseudomonas aeruginosa is a highly adaptable bacterium that thrives in broad range of ecological niches and can infect multiple hosts as diverse plants, nematodes mammals. In humans, it an important opportunistic pathogen. This wide adaptability correlates with its genetic diversity. this study, we used deep-sequencing approach to explore the complement small RNAs (sRNAs) P. number such regulatory molecules previously identified organism relatively low, considering genome size, phenotypic...
Abstract The dynamic conformation of RNA molecules within living cells is key to their function. Recent advances in probing the structurome vivo, including use SHAPE (Selective 2′-Hydroxyl Acylation analyzed by Primer Extension) or kethoxal reagents DMS (dimethyl sulfate), provided unprecedented insights into architecture cell. Here, we report establishment lead a global structuromics approach. In order elucidate transcriptome-wide landscape enteric pathogen Yersinia pseudotuberculosis,...
Pneumococcal conjugate vaccines (PCVs) used in childhood vaccination programs have resulted replacement of vaccine-type with nonvaccine-type pneumococci carriage and invasive pneumococcal disease (IPD). A vaccine based on highly conserved protective antigens is urgently needed. Here, we performed intranasal immunization mice membrane particles (MPs) to mimic natural nasopharyngeal immunization. MP gave excellent serotype-independent protection against IPD that was antibody dependent but...
Molecular mechanisms by which sex steroids interact with P. aeruginosa to modulate its virulence have yet be reported. Our work provides the first characterization of a steroid-induced membrane stress mechanism promoting virulence, includes release proinflammatory outer vesicles, resulting in inflammation, host tissue damage, and reduced bacterial clearance. We further demonstrate that at nanomolar (physiological) concentrations, male female promote clinical strains based on their dynamic...
The city of Copenhagen currently pursues a very ambitious plan to make the ‘cloudburst proof’ within next 30 years. cloudburst management has potential support city’s aim become more green, liveable, and sustainable. In this study, we assessed stormwater system designs using Three Point Approach (3PA) as framework, where an indicator value for each domain was calculated state-of-the-art modelling techniques. We demonstrated methodology on scenarios representing sequential enhancements...
Staphylococcus aureus is a Gram-positive bacterial pathogen of global concern and leading cause infections worldwide. Asymptomatic carriage S. on the skin in anterior nares common recognized as predisposing factor to invasive infection. Transition from state that infection often accompanied by temperature upshift approximately 33°C 37°C. Such shift known other pathogens influence gene expression, resulting increased production factors promote survival or virulence within host. One mechanism...
Abstract Eine korrekte und präzise Faltung der Sekundär‐ Tertiärstruktur ist von entscheidender Bedeutung für die zelluläre Funktionalität Ribonukleinsäuren (RNA). Faltungsstudien RNA wurden allerdings überwiegend in vitro durchgeführt, ohne Möglichkeit solche Experimente vivo zu validieren. In dieser Arbeit konnten wir Faltungsstabilität einer RNA‐Haarnadel direkt lebenden Säugerzellen auflösen. Die Ergebnisse zeigen, dass innerhalb Zelle mit verdünnten physiologischen Pufferlösung...
Increasing evidence has demonstrated that regulatory RNA elements such as riboswitches (RS) play a pivotal role in the fine-tuning of bacterial gene expression. In this study, we investigated and characterized novel transcriptional thiamine pyrophosphate (TPP) RS obligate human pathogen N. meningitidis MC58 (serogroup B). This is located 5´ untranslated region upstream thiC gene, encoding protein involved TPP biosynthesis, an essential cofactor for all living beings. Primer extension...
After this article [1] was published, concerns were raised about the reported western blot results.Specifically:• There appear to be vertical discontinuities between lanes in following panels: Figs 1 (after lane 3), 4A 2), 7C 1) and 7D 2).For each figure panel, discontinuity reflects where nonadjacent image fragments from same exposure spliced together preparing figure.Figs 1, 4 7 below have been updated clarify that panels represent blots, original images are provided S1-S4 Files.• appears...