A5078503780- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Studies on Chitinases and Chitosanases
- Invertebrate Immune Response Mechanisms
- Insect behavior and control techniques
- Multiple Myeloma Research and Treatments
- Streptococcal Infections and Treatments
- Insect Resistance and Genetics
- Antifungal resistance and susceptibility
- Antibiotics Pharmacokinetics and Efficacy
- Urinary Tract Infections Management
- Sperm and Testicular Function
- Pneumonia and Respiratory Infections
- Clostridium difficile and Clostridium perfringens research
- Bacterial biofilms and quorum sensing
- Amoebic Infections and Treatments
- Hemoglobinopathies and Related Disorders
- Infective Endocarditis Diagnosis and Management
- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Enzyme Production and Characterization
- Bacterial Infections and Vaccines
- Antimicrobial Resistance in Staphylococcus
- Shoulder Injury and Treatment
- Clinical Laboratory Practices and Quality Control
Azienda Ospedaliera San Gerardo
2019-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2023-2024
University of Milano-Bicocca
2015-2022
Azienda Socio Sanitaria Territoriale Lariana
2019-2021
University of Milan
2006-2018
We retrospectively analyzed the antimicrobial data of Enterobacter spp. strains isolated from hospitalized subjects and outpatients over 20 years (2000-2019). A total 2277 non-duplicate isolates, 1037 (45%) 1240 (55%), were retrieved. Most samples are infections urinary tract. Considering aerogenes, now classified as Klebsiella cloacae, representing more than 90% all except for aminoglycosides fluroquinolones, which showed significant antibiotic decreasing trends (p < 0.01), none other...
Beta-N-acetylhexosaminidases belonging to the glycosyl hydrolase 20 (GH20) family are involved in removal of terminal beta-glycosidacally linked N-acetylhexosamine residues. These enzymes, widely distributed microorganisms, animals and plants, many important physiological pathological processes, such as cell structural integrity, energy storage, pathogen defence, viral penetration, cellular signalling, fertilization, development carcinomas, inflammatory events lysosomal storage diseases....
Sperm surface beta-N-acetylhexosaminidases are among the molecules mediating early gamete interactions in invertebrates and vertebrates, including man. The plasma membrane of Drosophila spermatozoa contains two beta-N-acetylhexosaminidases, DmHEXA DmHEXB, which required for egg fertilization. Here, we demonstrate that three putative melanogaster genes predicted to code Hexo1, Hexo2, fdl, all expressed male germ line. fdl codes a homolog alpha-subunit mammalian lysosomal...
Abstract Drosophila is emerging as a model organism to investigate egg fertilization in insects and the possible conservation of molecular mechanisms gamete interactions demonstrated higher organisms. This study shows that spermatozoa several species belonging melanogaster group have plasma membrane associated α‐ L ‐fucosidase with features common ‐fucosidases from sperm other animals, including mammals. The enzyme has been purified completely characterized D. ananassae , because its...
Previous studies have suggested that iron-deficiency anemia affects glycosylated hemoglobin (HbA1c) measurements, but the results were contradictory. We conducted a retrospective case-control study to determine effects of iron deficiency on HbA1c levels. Starting with large computerized database Italian Hospital Desio, including data from 2000 2016, all non-pregnant individuals older than 12 years age at least one measurement HbA1c, cell blood count, ferritin, and fasting glucose same date...
Antimicrobial resistance is a worldwide problem, and in Enterobacteriaceae, particularly Escherichia coli Klebsiella pneumoniae, critical threat to human health. Inappropriate unnecessary use of antibiotics health care the most common cause for development spread antimicrobial resistance. In this work, we retrospectively analyzed data E. strains isolated from midstream urinary samples over 20-year period (2000-2019). The aim was provide useful information clinicians prescribe more...
Hypersensitivity pneumonitis (HP) represents the third common interstitial lung disease caused by an exaggerated immune response following inhalation of organic and/or chemical environmental antigens. The aim this study was to determine cut-off values specific IgG antibodies (named precipitins) and their association with clinical data in diagnosis HP. In 10-year retrospective study, concentrations against six antigens, Penicillium chrysogenum/notatum, Aspergillus fumigatus, Alternaria...
Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) is becoming a popular technology in clinical microbiology. It fast and highly specific method for the routine identification of micro-organisms. In this study, we evaluated suitability dermatophyte after only 2 days colony growth using MALDI-TOF MS. Two protein extraction protocols were also consisting either formic acid alone or ethanol-formic acid-acetonitrile to achieve complete extraction....
The correlation of clinical, radiological and laboratory findings patients at admission in the Emergency Department (ED) with clinical severity risk mortality was investigated. Adult coronavirus disease 2019 (COVID-19) hospitalized March 2020 Desio Hospital, Lombardy, were retrospectively included study, categorized terms adverse outcome. Out 175 enrolled, 79% presented one or more comorbidities, cardiovascular being most frequent (62%). More than half showed lymphocytopenia 20%...
Abstract We have studied the presence of four sperm glycosidases, α‐mannosidase, α‐ l ‐ fucosidase and two β‐hexosaminidase isoforms, in 11 species genus Drosophila spanning approximately an evolutionary 60 MY period, Scaptodrosophila lebanonensis , belongin g to ancestor . These enzymes had been previously identified melanogaster as putative receptors for glycoconjugates egg surface. Alpha‐mannosidase β‐hexosaminidases are intrinsic proteins plasma membrane closely related D. well divergent...
Modern blood gas analyzers are not able to identify hemolysis, lipemia and icterus; therefore, the aim of this study was assess influence hemolysis on samples. Blood analysis represents an essential part in diagnosis treatment critically ill patients, including those affected by pandemic coronavirus disease 2019 (COVID-19). Hemolysis, lipemia, icterus, causes clinical misinterpretation laboratory tests. A total 1244 specimens were collected over a one-week period from different wards,...
Staphylococcus epidermidis is an opportunistic pathogen and a frequent cause of nosocomial infections. In this work, we show that, among 51 S. isolates from Italian hospital, only minority displayed biofilm formation, regardless their isolation source (peripheral blood, catheter, or skin wounds); however, the biofilm-producing isolates, those catheters were most efficient in formation. Interestingly, including strong producers production levels PIA (polysaccharide intercellular adhesin),...
Immunoglobulin D (IgD) myeloma represents an uncommon subtype of multiple (MM), accounting for 1–2% cases. Subjects affected by IgD MM have been demonstrated to inferior outcome and survival compared those with other subtypes. A retrospective study was conducted on 15 patients (9 males 6 females) diagnosed from 2008 2022 MM, in order investigate the clinical biochemical features at moment diagnosis, cytogenetic alterations, times. The median age 69 years, higher frequencies bone lesions,...
Dientamoebiasis is globally distributed and detected in a large number of subjects with diarrhea, abdominal discomfort, flatulence, fatigue loss appetite. The life cycle transmission Dientamoeba fragilis are poorly understood. Microscopic examination permanent stained smears traditionally employed to diagnose the infection. However, this approach time-consuming success detecting D. depends on microscopist's experience. Hence, only few laboratories routinely carry out tests for fragilis....