Anna Di Matteo
A5088394304- Cancer, Lipids, and Metabolism
- Diet and metabolism studies
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Cell Adhesion Molecules Research
- Cancer-related molecular mechanisms research
- Metabolomics and Mass Spectrometry Studies
- Molecular Biology Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Pharmaceutical Practices and Patient Outcomes
- Mesenchymal stem cell research
- Anesthesia and Neurotoxicity Research
- Microbial Metabolism and Applications
- bioluminescence and chemiluminescence research
- Sarcoma Diagnosis and Treatment
- Opioid Use Disorder Treatment
- Protein Hydrolysis and Bioactive Peptides
- Protein Kinase Regulation and GTPase Signaling
- Liver physiology and pathology
- Alzheimer's disease research and treatments
- ATP Synthase and ATPases Research
- Neuropeptides and Animal Physiology
- Neuroinflammation and Neurodegeneration Mechanisms
National Research Council
2015-2024
Istituto di Genetica Molecolare
2015-2023
Angelini Pharma (Italy)
2013-2021
Abstract Cancer cells exhibit metabolic plasticity to meet oncogene-driven dependencies while coping with nutrient availability. A better understanding of how systemic metabolism impacts the accumulation metabolites that reprogram tumor microenvironment (TME) and drive cancer could facilitate development precision nutrition approaches. Using Hi-MYC prostate mouse model, we demonstrated an obesogenic high-fat diet (HFD) rich in saturated fats accelerates c-MYC–driven invasive through...
Abstract Vascular lumen formation is a fundamental step during angiogenesis; yet, the molecular mechanisms underlying this process are poorly understood. Recent studies have shown that neural and vascular systems share common anatomical, functional similarities. Here we show organization of endothelial controlled at post-transcriptional level by alternative splicing (AS) regulator Nova2, which was previously considered to be cell-specific. Nova2 expressed angiogenesis its depletion disrupts...
The biological players involved in angiogenesis are only partially defined. Here, we report that endothelial cells (ECs) express a novel isoform of the cell-surface adhesion molecule L1CAM, termed L1-ΔTM. splicing factor NOVA2, which binds directly to L1CAM pre-mRNA, is necessary and sufficient for skipping transmembrane domain ECs, leading release soluble latter exerts high angiogenic function through both autocrine paracrine activities. Mechanistically, L1-ΔTM-induced requires fibroblast...
Abstract The Netrin-1 receptor UNC5B is an axon guidance regulator that also expressed in endothelial cells (ECs), where it finely controls developmental and tumor angiogenesis. In the absence of Netrin-1, induces apoptosis blocked upon binding. Here, we identify splicing isoform (called UNC5B-Δ8) exclusively by ECs generated through exon skipping NOVA2, alternative factor regulating vascular development. We show UNC5B-Δ8 a constitutively pro-apoptotic insensitive to required for specific...
One of the hallmarks Alzheimer's disease (AD), most common age-related neurodegenerative pathology, is abnormal extracellular deposition neurotoxic amyloid-β (Aβ) peptides that accumulate in senile plaques. Aβ aggregates are toxic to neurons and thought contribute neuronal loss. Evidence indicates inflammation involved pathophysiology AD, activation glial cells by a variety factors, including Aβ, appears be central event. Among molecules produced during associated with death, CCL2, also...
Angiogenesis is crucial for cancer progression. While several anti-angiogenic drugs are in use treatment, their clinical benefits unsatisfactory. Thus, a deeper understanding of the mechanisms sustaining vessel growth fundamental to identify novel biomarkers and therapeutic targets. Alternative splicing (AS) an essential modifier human proteome diversity. Nevertheless, AS contribution tumor vasculature development poorly known. The Neuro-Oncological Ventral Antigen 2 (NOVA2) critical...
Bipolar disorders still represent a global unmet medical need and pose requirement for novel effective treatments. In this respect, glycogen synthase kinase 3β (GSK-3β) aberrant activity has been linked to the pathophysiology of several disease conditions, including mood disorders. Therefore, development GSK-3β inhibitors with good in vivo efficacy safety profile associated high brain exposure is required. Accordingly, we have previously reported selective indazole-based GSK-3 inhibitor 1,...
Alternative splicing (AS) plays an important role in expanding the complexity of human genome through production specialized proteins regulating organ development and physiological functions, as well contributing to several pathological conditions. How AS programs impact on signaling pathways controlling endothelial cell (EC) functions vascular is largely unknown. Here we identified, RNA-seq, changes mRNA steady-state levels ECs caused by neuro-oncological ventral antigen 2 (Nova2), a key...
Neuropathic pain is a chronic debilitating condition caused by injury or disease of the nerves somatosensory system. Although several therapeutic approaches are recommended, none has emerged as an optimal treatment leaving need for developing more effective therapies. Given small number approved drugs and their limited clinical efficacy, combining with different mechanisms action frequently used to yield greater efficacy. We demonstrate that combination trazodone, multifunctional drug major...
Breast cancer is the most frequently occurred type and second cause of death in women worldwide. Alternative splicing (AS) process that generates more than one mRNA isoform from a single gene, it plays major role expanding human protein diversity. Aberrant AS contributes to breast metastasis resistance chemotherapeutic interventions. Therefore, identifying cancer-specific isoforms prerequisite for therapeutic interventions intended correct aberrantly expressed events. Here, we performed...
<p>Supplementary Figure S7 shows the output of mouse-based deconvolution models ImmuCC and mMCP.</p>
<p>Supplementary Figure S2 shows the effect of obesogenic HFD on AP and VP lobes, cell proliferation rate in MYC-transformed DLP, WT prostate lobes.</p>
<p>Supplementary Figure S1 shows the systemic effects of HFD.</p>
<p>Supplementary Figure S2 shows the effect of obesogenic HFD on AP and VP lobes, cell proliferation rate in MYC-transformed DLP, WT prostate lobes.</p>
<p>Supplementary Figure S6 includes scRNA-seq data showing that Luminal (high Ly6d) cells in MYC-transformed DLP display glycolytic features.</p>
<p>Supplementary Figure S5 shows the output of ESTIMATE and PUREE methods to assess tumor purity.</p>
<p>Supplementary Figure S4 shows the effect of obesogenic high-fat diet on glycolytic enzymes and HIF-1 alpha in vivo.</p>
<div>Abstract<p>Cancer cells exhibit metabolic plasticity to meet oncogene-driven dependencies while coping with nutrient availability. A better understanding of how systemic metabolism impacts the accumulation metabolites that reprogram tumor microenvironment (TME) and drive cancer could facilitate development precision nutrition approaches. Using Hi-MYC prostate mouse model, we demonstrated an obesogenic high-fat diet (HFD) rich in saturated fats accelerates c-MYC–driven...
<p>Supplementary Figure S3 shows a graphical representation of key metabolic pathways altered by MYC alone or obesogenic HFD in WT and MYC-transformed DLP.</p>