A5102748150- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Lymphoma Diagnosis and Treatment
- Melanoma and MAPK Pathways
- CAR-T cell therapy research
- Anodic Oxide Films and Nanostructures
- Cancer-related molecular mechanisms research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer Immunotherapy and Biomarkers
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Chemokine receptors and signaling
- Immune cells in cancer
- Ferroptosis and cancer prognosis
- Immunotherapy and Immune Responses
- Chronic Lymphocytic Leukemia Research
- Peptidase Inhibition and Analysis
- Axon Guidance and Neuronal Signaling
- Cancer, Lipids, and Metabolism
- Bladder and Urothelial Cancer Treatments
- Genetic factors in colorectal cancer
- Computational Drug Discovery Methods
- Biochemical effects in animals
- Innovative Microfluidic and Catalytic Techniques Innovation
- RNA modifications and cancer
University of Brescia
2017-2025
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
2019-2025
Brescia University
2024
Mitogen-activated protein kinase (MAPK) pathway antagonists induce profound clinical responses in advanced cutaneous melanoma, but complete remissions are frustrated by the development of acquired resistance. Before resistance emerges, adaptive establish a mutation-independent drug tolerance. Antagonizing these could improve effects, thereby thwarting emergence In this study, we reveal that inflammatory niches consisting tumor-associated macrophages and fibroblasts contribute to treatment...
Urothelial bladder cancer (UBC) are classified into luminal and basal subtypes showing distinct molecular features clinical behaviour. Recent in silico data have proposed the activation on Signal Transducer Activator of Transcription 3 (STAT3) as relevant transcription factor UBC. To answer this question, we combined retrospective analysis samples, functional assays cell lines, interrogation public UBC datasets a murine model basal-type Immunohistochemistry cohort uncovered that STAT3 Y705...
Prostate cancer (PCa) is a leading cause of death in the male population commonly treated with androgen deprivation therapy that often relapses as androgen-independent and aggressive castration-resistant prostate (CRPC). Ferroptosis recently described form cell requires abundant cytosolic labile iron to promote membrane lipid peroxidation which can be induced by agents inhibit glutathione peroxidase-4 activity such RSL3. Exploiting vitro vivo human murine PCa models multistage transgenic...
Cerebral organoids (cORGs) obtained from induced pluripotent stem cells (iPSCs) have become significant instruments for investigating human neurophysiology, with the possibility of simulating diseases and enhancing drug discovery. The current approaches require a strict process manual inclusion in animal-derived matrix Matrigel® are challenged by unpredictability, operators' skill expertise, elevated costs, restricted scalability, impeding their extensive applicability translational...
Abstract The Netrin-1 receptor UNC5B is an axon guidance regulator that also expressed in endothelial cells (ECs), where it finely controls developmental and tumor angiogenesis. In the absence of Netrin-1, induces apoptosis blocked upon binding. Here, we identify splicing isoform (called UNC5B-Δ8) exclusively by ECs generated through exon skipping NOVA2, alternative factor regulating vascular development. We show UNC5B-Δ8 a constitutively pro-apoptotic insensitive to required for specific...
Methylglyoxal (MGO), a highly reactive precursor of advanced glycation end products (AGEs), is endogenously produced and prevalent in various ultra-processed foods. MGO has emerged as significant implicated the pathogenesis type 2 diabetes neurodegenerative diseases. To date, effects dietary on intestine have been limited explored. Thus, this study investigates impact prolonged oral administration MGOs gut health aged mice. Aged mice received chronically (100 mg/kg/day) for 4 weeks....
Angiogenesis is crucial for cancer progression. While several anti-angiogenic drugs are in use treatment, their clinical benefits unsatisfactory. Thus, a deeper understanding of the mechanisms sustaining vessel growth fundamental to identify novel biomarkers and therapeutic targets. Alternative splicing (AS) an essential modifier human proteome diversity. Nevertheless, AS contribution tumor vasculature development poorly known. The Neuro-Oncological Ventral Antigen 2 (NOVA2) critical...
The endothelin-1 (ET-1) receptors were recently found to mediate pro-survival functions in multiple myeloma (MM) cells response autocrine ET-1. This study investigated the effectiveness of macitentan, a dual ET-1 receptor antagonist, MM treatment, and mechanisms underlying its activities. Macitentan affected significantly cell (RPMI-8226, U266, KMS-12-PE) survival pro-angiogenic cytokine release by down-modulating ET-1-activated MAPK/ERK HIF-1α pathways, respectively. silencing abrogated...
CRCMSS/pMMR contain a significantly increased fraction of TREM2+ macrophages (TAMs) and CD66+ neutrophils (TANs) together with decrease CD4-CD8-CD3+ double negative T lymphocytes (DNTs); no differences were revealed by the analysis myeloid plasmacytoid dendritic cell populations. A tumor-infiltrating T-cells display an exhausted phenotype, co-expressing PD-1 TIM-3. Remarkably, expression PD-L1 on fresh tumor cells TAMs was undetectable even after in vitro stimulation interferon-γ. These...
BRAF is one of the most common mutated kinases detected in human cancer, particularly cases primary cutaneous melanomas (PCM). Mutations proto-oncogene, at p.V600 codon, has been more than 50% and metastatic melanoma cells clinical samples. These mutations lead to constitutive signal transduction MAPK (mitogen activating proteins kinase) pathway. This signaling pathway can be modified reversed by introduction a combination highly selective MEK inhibitors BRAFV600 melanomas. In addition...
Abstract Tumor-associated macrophages (TAMs) are the predominant immune population within tumor microenvironment (TME), playing a key role in promoting growth and establishing an immunosuppressive environment that facilitate evasion. Here we report receptor CD300e is highly expressed by TAM colorectal (CRC) drives their pro-tumorigenic, correlating with reduced expression of MHC-II molecules, essential for antigen presentation. In vitro, CD300e-deficient exhibit enhanced pro-inflammatory...
Abstract The B cell receptor (BCR) is essential for mature lymphomas, serving as therapeutic target. Here, we show that high-grade lymphomas with MYC and BCL2 rearrangements (HGBCL-DH- ) predominantly exhibit immunoglobulin heavy (IGH) chain silencing, leading to BCR shutdown. HGBCL-DH- undetectable IGH (IGH UND differ from IGH-expressing counterparts germinal center-zone gene programs, expression T infiltration. While + prefer IGM/IG-Kappa expression, have completed class-switching,...
Neutrophils (PMNs) are key players of innate immune responses through the release cytoplasmic granule content and formation neutrophil extracellular traps (NETs). RNASET2 is an acidic ribonuclease, recently proposed as alarmin signal associated with inflammatory responses. Here we show that, along maturation cascade, expressed in segmented mature PMNs. In human PMNs, colocalized primary tertiary granules was found to be NETs following PMA or Nigericin stimulation. Similarly, activation PMNs...
<p>Supplementary Materials and Methods</p>
<p>Supplementary Materials and Methods</p>
Abstract Crohn’s disease (CD) is a chronic transmural inflammation of intestinal segments caused by dysregulated interaction between microbiome and gut immune system. Recurrent/relapsing CD resistance to medical treatments result in complications requiring surgery. High-dimensional single-cell profiling approaches, such as scRNA-seq mass cytometry, have been recently performed on specimens from patients with IBD controls. However, most these studies analyzed whole mucosal biopsies or the...