A5103276257- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Metabolism, Diabetes, and Cancer
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- Cell Adhesion Molecules Research
- Genomics and Chromatin Dynamics
- Gut microbiota and health
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Cancer, Stress, Anesthesia, and Immune Response
- Diet and metabolism studies
- Health, Environment, Cognitive Aging
- Cancer-related molecular mechanisms research
- Nuclear Structure and Function
- Head and Neck Cancer Studies
- Cancer Genomics and Diagnostics
- Peptidase Inhibition and Analysis
- Cell death mechanisms and regulation
- Cancer Cells and Metastasis
- Protease and Inhibitor Mechanisms
- 3D Printing in Biomedical Research
- RNA Research and Splicing
Tethys Research Institute
2023
European Institute of Oncology
2007-2021
Institute for Experimental Endocrinology and Oncology
2012-2015
IFOM
2006-2014
Institute of Experimental Endocrinology of the Slovak Academy of Sciences
2012
University of Naples Federico II
2012
Center for Genomic Science
2011
Background Deubiquitinating enzymes (DUBs) are proteases that process ubiquitin (Ub) or ubiquitin-like gene products, remodel polyubiquitin(-like) chains on target proteins, and counteract protein ubiquitination exerted by E3 ubiquitin-ligases. A wealth of studies has established the relevance DUBs to control physiological processes whose subversion is known cause cellular transformation, including cell cycle progression, DNA repair, endocytosis signal transduction. Altered expression might,...
Abstract USP6NL, also named RN-tre, is a GTPase-activating protein involved in control of endocytosis and signal transduction. Here we report that USP6NL overexpressed breast cancer, mainly the basal-like/integrative cluster 10 subtype. Increased levels were accompanied by gene amplification associated with worse prognosis METABRIC dataset, retaining prognostic value multivariable analysis. High cancer cells delayed degradation EGFR, causing chronic AKT (protein kinase B) activation. In...
Tumor initiation and progression provide a multitude of occasions for the generation DNA damage consequent activation response (DDR) pathway. DDR signaling involves engagement key factors such as ATM, CHK2, 53BP1 phosphorylation histone H2AX (γ-H2AX). The systematic study in human tumors normal tissues by high-throughput tissue microarrays revealed that ATM γ-H2AX were engaged cancer but extent their was strongly affected organ cell type involved, whereas loss most consistent feature among...
The biological players involved in angiogenesis are only partially defined. Here, we report that endothelial cells (ECs) express a novel isoform of the cell-surface adhesion molecule L1CAM, termed L1-ΔTM. splicing factor NOVA2, which binds directly to L1CAM pre-mRNA, is necessary and sufficient for skipping transmembrane domain ECs, leading release soluble latter exerts high angiogenic function through both autocrine paracrine activities. Mechanistically, L1-ΔTM-induced requires fibroblast...
Non‐small cell lung cancer (NSCLC) is the main cause of cancer‐related death worldwide and new therapeutic strategies are urgently needed. In this study, we have characterized a panel NSC lines for expression coiled‐coil‐domain containing 6 (CCDC6), tumor suppressor gene involved in apoptosis DNA damage response. We show that low CCDC6 protein levels associated with weak response to number Rad51 positive foci. Moreover, deficient cells defects repair via homologous recombination. accordance...
CCDC6 gene product is a pro-apoptotic protein substrate of ATM, whose loss or inactivation enhances tumour progression. In primary tumours, the impaired function has been ascribed to rearrangements and somatic mutations in several neoplasia. Recently, low levels protein, NSCLC, have correlated with tumor prognosis. However, mechanisms responsible for variable tumors not described yet.We show that turnover regulated cell cycle dependent manner. undergoes cyclic variation phosphorylated status...
The natural history of oral squamous cell carcinomas (OSCCs) is variable and difficult to predict. This study aimed assess the value expression poly(ADP-ribose) polymerase 1 (PARP-1), chromatin assembly factor-1 (CAF-1)/p60 stem markers CD133, CD166, CD44, CD44v6 nestin as outcome progression-free survival in OSCC patients.Clinical data were collected from 66 patients (41 male 25 female, aged 29-92 years) who underwent surgery for tongue, floor, lips, palate. During follow-up (range: 12-131...
CCDC6 was originally identified in chimeric genes caused by chromosomal translocation involving the RET proto-oncogene some thryoid tumors mostly upon ionizing radiation exposure. Recognised as a pro-apoptotic phosphoprotein that negatively regulates CREB1-dependent transcription, is an ATM substrate responsive to genotoxic stress. Here we report following stress, loss or inactivation of cancers carry fusion, accelerates dephosphorylation pH2AX S139, resulting defective G2 arrest and...
Cellular senescence has been widely recognized as a tumor suppressing mechanism that acts barrier to cancer development after oncogenic stimuli. A prominent in vivo model of the is represented by nevi, which are composed melanocytes that, an initial phase proliferation induced activated oncogenes (most commonly BRAF), blocked state cellular senescence. Transformation melanoma occurs when genes involved controlling mutated or silenced and cells reacquire capacity proliferate. Pirin (PIR)...
Abstract How cells move chemotactically remains a major unmet challenge in cell biology. Emerging evidence indicates that for interpreting noisy, shallow gradients of soluble cues system must behave as an excitable process. Here, through RNAi-based, high-content screening approach, we identify RAB35 necessary the formation growth factors (GFs)-induced waves circular dorsal ruffles (CDRs), apically restricted actin-rich migratory protrusions. is sufficient to induce recurrent and polarized...
Abstract Background Pirin (PIR) is a highly conserved nuclear protein originally isolated as an interactor of NFI/CTF1 transcription/replication factor. It member the functionally diverse cupin superfamily and its activity has been linked to different biological molecular processes, such regulation transcription, apoptosis, stress response enzymatic processes. Although precise role in these functions not yet defined, PIR expression known be deregulated several human malignancies. Results We...
Abstract Background DNA damage response has been clearly described as an anti-cancer barrier in early human tumorigenesis. Moreover, interestingly, testicular germ cell tumors (TGCTs) have reported to lack the Damage Response (DDR) pathway activation. CCDC6 is a pro-apoptotic phosphoprotein substrate of kinase ataxia telangectasia mutated (ATM) able sustain checkpoint genotoxic stress and commonly rearranged malignancies upon fusion with different partners. In our study we sought determine...
Lung cancer is still the leading cause of cancer-related death worldwide. Interest growing towards early detection and advances in liquid biopsy to isolate circulating tumor cells (CTCs). This pilot study aimed detect epithelial CTCs peripheral blood early-stage non-small cell lung (NSCLC) patients. We used Smart BioSurface® (SBS) slide, a nanoparticle-coated slide able immobilize viable nucleated cellular fraction without pre-selection preserve integrity. Forty patients undergoing resection...
CCDC6 was originally identified in chimeric genes as caused by chromosomal translocation involving the RET protooncogene some thyroid tumors. Recognised a 65 kDa pro-apoptotic phosphoprotein, has been enrolled an ATM substrate that contribute to protect genome integrity modulating PP4c activity response genotoxic stress. Recently, repressor of CREB1-dependent transcription. Sumoylation emerged important mechanism transcriptional control. Here, we report identification and characterization...
Abstract Background: Maspin is a member of the serpin family protease inhibitors that thought to act as tumor suppressor in several cancer types, including melanoma. Recent studies have shown maspin sub-cellular distribution may impact its effect on disease outcome. Similar are missing Methods: Using 3 different tissue microarrays (TMA), expression was evaluated by two pathologists blinded manner 150 primary lesions and 106 metastasis obtained melanoma patients. nuclear cytoplasmic...
<p>USP6NL overexpression increases AKT phosphorylation</p>
<div>Abstract<p>USP6NL, also named RN-tre, is a GTPase-activating protein involved in control of endocytosis and signal transduction. Here we report that USP6NL overexpressed breast cancer, mainly the basal-like/integrative cluster 10 subtype. Increased levels were accompanied by gene amplification associated with worse prognosis METABRIC dataset, retaining prognostic value multivariable analysis. High cancer cells delayed degradation EGFR, causing chronic AKT (protein kinase B)...
<p>USP6NL silencing does not affect the USP6NL-low H1299 cell line</p>
<div>Abstract<p>USP6NL, also named RN-tre, is a GTPase-activating protein involved in control of endocytosis and signal transduction. Here we report that USP6NL overexpressed breast cancer, mainly the basal-like/integrative cluster 10 subtype. Increased levels were accompanied by gene amplification associated with worse prognosis METABRIC dataset, retaining prognostic value multivariable analysis. High cancer cells delayed degradation EGFR, causing chronic AKT (protein kinase B)...