A5036873809- Glioma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Single-cell and spatial transcriptomics
- interferon and immune responses
- DNA Repair Mechanisms
- PARP inhibition in cancer therapy
- MicroRNA in disease regulation
- Immune cells in cancer
- Hedgehog Signaling Pathway Studies
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- Circular RNAs in diseases
- Ferroptosis and cancer prognosis
- RNA Research and Splicing
- Optimism, Hope, and Well-being
- Blood properties and coagulation
- Virus-based gene therapy research
- Cancer Cells and Metastasis
- Genomics and Chromatin Dynamics
- Erythropoietin and Anemia Treatment
- Lipid metabolism and disorders
Spanish National Cancer Research Centre
2015-2025
AstraZeneca (United Kingdom)
2022-2024
University of Liège
2024
Centro de Investigación del Cáncer
2017-2023
Memorial Sloan Kettering Cancer Center
2007-2016
The University of Texas MD Anderson Cancer Center
2016
Universidade Federal do Rio Grande do Sul
2016
Jackson Laboratory
2016
University of Copenhagen
2013
Fujita Health University Hospital
2013
The miR-17∼92 cluster is frequently amplified or overexpressed in human cancers and has emerged as the prototypical oncogenic polycistron microRNA (miRNA). a direct transcriptional target of c-Myc, experiments mouse model B-cell lymphomas have shown cooperation between these two oncogenes. However, both molecular mechanism underlying this individual miRNAs that are responsible for it unknown. By using conditional knockout allele , we show here sustained expression endogenous required to...
Abstract Cancer response to immunotherapy depends on the infiltration of CD8 + T cells and presence tumor-associated macrophages within tumors. Still, little is known about determinants these factors. We show that LIF assumes a crucial role in regulation cell tumor infiltration, while promoting protumoral macrophages. observe blockade tumors expressing high levels decreases CD206, CD163 CCL2 induces CXCL9 expression The releases epigenetic silencing triggering infiltration. combination...
Abstract Temozolomide (TMZ) is an oral alkylating agent used for the treatment of glioblastoma and now becoming a chemotherapeutic option in patients diagnosed with high-risk low-grade gliomas. The O-6-methylguanine-DNA methyltransferase (MGMT) responsible direct repair main TMZ-induced toxic DNA adduct, O6-Methylguanine lesion. MGMT promoter hypermethylation currently only known biomarker TMZ response patients. Here we show that subset recurrent gliomas carries genomic rearrangements lead...
Gene rearrangement in the form of an intragenic deletion is primary mechanism oncogenic mutation epidermal growth factor receptor ( EGFR ) gene gliomas. However, incidence platelet-derived receptor-α PDGFRA these tumors unknown. We investigated locus -amplified gliomas and identified two rearrangements, including first case a fusion between kinase insert domain KDR VEGFRII gene, six cases Δ8, 9 , rearrangement. The mutant was common, being present 40% glioblastoma multiformes (GBMs) with...
The Hedgehog (Hh) signaling pathway plays an important role in embryonic patterning and development of many tissues organs as well maintaining repairing mature adults. Uncontrolled activation the Hh-Gli has been implicated developmental abnormalities several cancers, including brain tumors like medulloblastoma glioblastoma. Inhibition aberrant has, thus, emerged attractive approach for anticancer therapy; however, mechanisms that mediate vertebrates remain poorly understood. Here, we show...
The molecular basis underlying glioblastoma (GBM) heterogeneity and plasticity is not fully understood. Using transcriptomic data of human patient-derived brain tumor stem cell lines (BTSCs), classified based on GBM-intrinsic signatures, we identify the AP-1 transcription factor
Genetically engineered mouse models (GEMMs) are instrumental for modelling local and systemic features of complex diseases such as cancer. Non-invasive, longitudinal cell detection monitoring in tumors, metastases and/or the micro-environment is paramount to achieve a better spatiotemporal understanding cancer progression evaluate therapies preclinical studies. Bioluminescent fluorescent reporters marking tumor cells or their microenvironment valuable non-invasive vivo. Here we report...
Background The tumor microenvironment contains normal, non-neoplastic cells that may contribute to growth and maintenance. Within PDGF-driven murine gliomas, tumor-associated astrocytes (TAAs) are a large component of the microenvironment. function in glioma has not been fully elucidated; moreover, differences between these normal unknown. We therefore sought identify genes pathways increased TAAs relative also determine whether expression correlates with behavior. Methodology/Principal...
Glioblastoma is a lethal brain tumor that exhibits heterogeneity and resistance to therapy. Our understanding of homeostasis limited by lack genetic tools selectively identify states fate transitions. Here, we use glioblastoma subtype signatures construct synthetic tracing cassettes investigate at cellular molecular levels, in vitro vivo. Through locus control regions, demonstrate proneural hardwired identity, whereas mesenchymal an adaptive metastable cell state driven proinflammatory...
To accurately recapitulate the heterogeneity of human diseases, animal models require to recreate multiple complex genetic alterations. Here, we combine RCAS-TVA system with CRISPR-Cas9 genome editing tools for precise modeling tumors. We show that somatic deletion in neural stem cells a variety known tumor suppressor genes (Trp53, Cdkn2a, and Pten) leads high-grade glioma formation. Moreover, by simultaneous delivery pairs guide RNAs generate different gene fusions oncogenic potential,...