A5030123650- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Glioma Diagnosis and Treatment
- Hedgehog Signaling Pathway Studies
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Estrogen and related hormone effects
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Chromatin Remodeling and Cancer
- Bacterial Genetics and Biotechnology
- Cancer-related gene regulation
- Telomeres, Telomerase, and Senescence
- Genetics and Plant Breeding
- RNA and protein synthesis mechanisms
- Rice Cultivation and Yield Improvement
- Bacteriophages and microbial interactions
- Glutathione Transferases and Polymorphisms
- Postharvest Quality and Shelf Life Management
- Circular RNAs in diseases
- Receptor Mechanisms and Signaling
- Transgenic Plants and Applications
Oslo University Hospital
2020-2025
University of Copenhagen
2011-2022
Institute of Genomics and Integrative Biology
2020
University of Geneva
2006-2019
Spanish National Cancer Research Centre
2013
University of Southern Denmark
2005
Prokaryotic chromosomes code for toxin–antitoxin (TA) loci, often in multiple copies. In E.coli, experimental evidence indicates that TA loci are stress-response elements help cells survive unfavorable growth conditions. The first gene a operon codes an antitoxin combines with and neutralizes regulatory ‘toxin’, encoded by the second gene. RelE MazF toxins regulators of translation cleave mRNA function, interplay tmRNA, quality control expression. Here, we present results from exhaustive...
Estrogen receptor alpha (ER alpha) is a ligand-regulated transcription factor with broad range of physiological functions and one the most important classifiers in breast cancer. MicroRNAs (miRNAs) are small noncoding RNAs that have emerged as regulators gene expression plethora pathological processes. Upon binding 3' untranslated region (UTR) target mRNAs, miRNAs typically reduce their stability and/or translation. The ER mRNA has long UTR about 4.3 kb which been reported to bears...
ABSTRACT Toxin-antitoxin (TA) loci are common in free-living bacteria and archaea. TA encode a stable toxin that is neutralized by metabolically unstable antitoxin. The antitoxin can be either protein or an antisense RNA. So far, six different gene families, which the antitoxins proteins, have been identified. Recently, Makarova et al. (K. S. Makarova, N. V. Grishin, E. Koonin, Bioinformatics 22:2581-2584, 2006) suggested hicAB constitute novel family. Using locus of Escherichia coli K-12 as...
The Hedgehog (Hh) signaling pathway plays an important role in embryonic patterning and development of many tissues organs as well maintaining repairing mature adults. Uncontrolled activation the Hh-Gli has been implicated developmental abnormalities several cancers, including brain tumors like medulloblastoma glioblastoma. Inhibition aberrant has, thus, emerged attractive approach for anticancer therapy; however, mechanisms that mediate vertebrates remain poorly understood. Here, we show...
Different cellular receptors mediate the biological effects induced by estrogens. In addition to classical nuclear estrogen (ERs)-α and -β, also signals through seven-transmembrane G-protein-coupled receptor (GPR)-30. Using as a model system SkBr3 BT20 breast cancer cells lacking ER, regulation of GPR30 expression 17β-estradiol, selective ligand G-1, IGF-I, epidermal growth factor (EGF) was evaluated. Transient transfections with an plasmid encoding short 5′-flanking sequence gene revealed...
Human telomerase reverse transcriptase (hTERT) remains suppressed in most normal somatic cells. Resulting erosion of telomeres leads eventually to replicative senescence. Reactivation hTERT maintains and triggers progression >90% cancers. However, any direct causal link between regulation unclear. Here, we show that the telomere-repeat-binding-factor 2 (TRF2) binds promoter G-quadruplexes recruits polycomb-repressor EZH2/PRC2 complex. This is for H3K27 trimethylation at represses cancer as...
Short regulatory RNAs are widespread in bacteria, and many function through antisense recognition of mRNA. Among the best studied transcripts RNA antitoxins that repress toxin mRNA translation. The hok/sok locus plasmid R1 from Escherichia coli is an established model for antitoxin action. Base-pairing between hok Sok-antisense-RNA increases maintenance post-segregational-killing plasmid-free progeny cells. To test idea sequestration Sok-RNA activity could provide a novel antimicrobial...
Abstract In response to extracellular signals, many signalling proteins associated with the plasma membrane are sorted into endosomes. This involves endosomal fusion, which depends on complexes HOPS and CORVET. Whether how their subunits themselves modulate signal transduction is unknown. We show that Vps11 Vps18 (Vps11/18), two common of HOPS/CORVET complexes, E3 ubiquitin ligases. Upon overexpression Vps11/Vps18, we find perturbations ubiquitination in pathways. specifically demonstrate...
Activation of interferon genes constitutes an important anticancer pathway able to restrict proliferation cancer cells. Here, we demonstrate that the H3K9me3 histone methyltransferase (HMT) suppressor variegation 3-9 homolog 1 (SUV39H1) is required for acute myeloid leukemia (AML) and find its loss leads activation pathway. Mechanistically, show this occurs via destabilization a complex composed SUV39H1 two H3K9me2 HMTs, G9A GLP. Indeed, correlated with key genes, interference activities...
Background and Objectives: Glioblastoma (GBM) is among the most aggressive lethal primary brain tumors, characterized by high heterogeneity, invasive growth, resistance to conventional therapies. The 2021 WHO classification highlights importance of molecular diagnostics, integrating genetic, transcriptomic, epigenetic alterations alongside histological immunohistochemical criteria. Materials methods: Key regulators, including ATRX, OLIG2, MGMT, IDH2, play critical roles in chromatin...
Abstract Background To make sense out of gene expression profiles, such analyses must be pushed beyond the mere listing affected genes. For example, if a group genes persistently display similar changes in levels under particular experimental conditions, and proteins encoded by these interact function same cellular compartments, this could taken as very strong indicators for co-regulated protein complexes. One key requirements is having appropriate tools to detect regulatory patterns....
Abstract Tight regulatory mechanisms to maintain repression of human Telomerase (hTERT), the sole protein that synthesizes telomeres, is crucial for normal adult somatic cells. In contrast, enhanced telomerase activity and resulting pathological maintenance widely understood as causal in >90% cancers. These implicate underlying connecting hTERT regulation possibly through telomeric proteins, remain unclear. light recent work by us others showing non-telomeric function telomere-binding...
Abstract Glioblastoma multiforme (GBM) is one of the most aggressive types tumors with a poor response to standard treatment and median 5-year survival less than 5%. Therefore, there an urgent need for new treatments. Recently, large number genome-wide studies have shown that epigenetic modifiers are frequently deregulated in cancer. Using mouse GBM model, we performed vitro vivo shRNA screens identify regulators required tumorigenic process GBM. Among these ribosome hydroxylase Mina53 which...
<p>PDF file, 906K, Supplementary Materials and Methods Flow cytometry. Colony formation assay. Tables Table 1. siRNAs shRNAs constructs used in this study. 2. Expression primers ChIP 3. Antibodies Figures Figure S1. Analysis of siRNA screening for acetyltransferases. S2. Validation candidate genes from the screening. S3. Stable depletion Pcaf does not affect DNA synthesis NIH3T3 cells. S4. Inhibition PCAF expression affects neurosphere proliferation GBM543 S5. Depletion inhibitors to...