A5023076666- Virus-based gene therapy research
- Nitric Oxide and Endothelin Effects
- Gastroesophageal reflux and treatments
- Birth, Development, and Health
- Renin-Angiotensin System Studies
- Pregnancy and preeclampsia studies
- HIV Research and Treatment
- Cancer Research and Treatments
- Eosinophilic Esophagitis
- Hormonal Regulation and Hypertension
- Animal Nutrition and Physiology
- CAR-T cell therapy research
- Acute Myeloid Leukemia Research
- HIV/AIDS Research and Interventions
- Esophageal and GI Pathology
- RNA modifications and cancer
- Brucella: diagnosis, epidemiology, treatment
- RNA Research and Splicing
- Eicosanoids and Hypertension Pharmacology
- Viral Infectious Diseases and Gene Expression in Insects
- Reproductive System and Pregnancy
- RNA Interference and Gene Delivery
- Salivary Gland Tumors Diagnosis and Treatment
- HIV/AIDS drug development and treatment
- Radiomics and Machine Learning in Medical Imaging
Peking Union Medical College Hospital
2005-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2005-2025
West China Hospital of Sichuan University
2025
Sichuan University
2025
Hangzhou Academy of Agricultural Sciences
2021-2024
Academy of Medical Sciences
2014-2024
University of Pennsylvania
2022-2024
Rigshospitalet
2013-2023
Copenhagen University Hospital
2023
University of Copenhagen
2013-2022
Abstract Purpose: The purpose of this study was to examine the tumor specificity, cytotoxicity, and granulocyte macrophage colony-stimulating factor expression CG0070, a conditionally replicating oncolytic adenovirus, in human bladder transitional cell carcinoma (TCC) lines determine its antitumor efficacy TCC models. Experimental Design: Virus yield cytotoxicity assays were used specificity virus replication-mediated CG0070 panel primary cells vitro. Two s.c. one orthotopic xenograft models...
Abstract Background Plant roots are the primary site of perception and injury for saline-alkaline stress. The current knowledge stress transcriptome is mostly focused on saline (NaCl) only limited information alkaline (NaHCO 3 ) available. Results Using Affymetrix ® Soybean GeneChip , we conducted transcriptional profiling Glycine soja subjected to 50 mmol/L NaHCO treatment. In a total 7088 probe sets, 3307 were up-regulated 5720 down-regulated at various time points. number significantly...
The esophageal intraluminal baseline impedance may be used to evaluate the status of mucosa integrity. Esophageal acid exposure decreases impedance. We aimed compare in patients with various reflux events and different acid-related parameters, investigate relationships between epithelial histopathologic abnormalities impedance.A total 229 GERD 34 controls underwent 24-h multichannel pH monitoring (MII-pH monitoring), gastroendoscopy, completed a questionnaire (GerdQ). quantified...
BackgroundBrucellosis is the most common zoonotic infection in world. Brucellosis with nervous system involvement known as 'neurobrucellosis' (NB). The diagnosis of NB difficult because its clinical manifestations are non-specific and sensitivity routine culture tests low.MethodsNext-generation sequencing (NGS) cerebrospinal fluid (CSF) was used to detect pathogens patients clinically suspected central (CNS) infections at a tertiary referral center China between June 1, 2016 2017....
During transcription, most eukaryotic genes generate multiple alternative cleavage and polyadenylation (APA) sites, leading to the production of transcript isoforms with variable lengths in 3' untranslated region (3'UTR). In contrast somatic cells, male germ especially pachytene spermatocytes round spermatids, express a distinct reservoir mRNAs shorter 3'UTRs that are essential for spermatogenesis fertility. However, mechanisms underlying enrichment 3'UTR transcripts developing cells remain...
Tiller production in grass species is controlled by both axillary bud initiation and outgrowth, which may be regulated plant hormones. However, how gibberellic acid (GA) affects tillering perennial still unclear. This study aims to elucidate the roles underlying mechanisms of GA regulating tiller development. Tall fescue seedlings were treated with different concentrations GA3 foliar application, dose-dependent inhibitory effects on observed. (25 μM) slowed down transition from buds tillers...
Transcription factors PU.1 and CEBPA are required for the proper coordination of enhancer activity during granulocytic-monocytic (GM) lineage differentiation to form myeloid cells. However, precisely how these control chronology establishment is not known. Through integrated analyses dynamics, transcription factor binding, proximal gene expression successive stages murine GM-lineage differentiation, we unravel distinct kinetics by which coordinate GM activity. We find no evidence a...
Cancer sequencing studies have implicated regulators of pre-mRNA splicing as important disease determinants in acute myeloid leukemia (AML), but the underlying mechanisms remained elusive. We hypothesized that "non-mutated" may also play a role AML biology and therefore conducted an vivo shRNA screen mouse model CEBPA mutant AML. This has led to identification regulator RBM25 novel tumor suppressor. In multiple human leukemic cell lines, knockdown promotes proliferation decreases apoptosis....
Background: Lutein is the primary macular pigment with an favorable effect on treatment of age-related degeneration (AMD). However, poor water solubility lutein hinders its absorption and delivery. In this study, a penetratin-modified nanoemulsion in-situ gel (GEL) was prepared for AMD.Methods: A (NE) modified penetratin (P-NE) to improve penetration. The evaluated by cell uptake intraocular distribution assays. dry AMD model induced using NaIO3, therapeutic electroretinography, number...
Nitric oxide (NO) is an important negative modulator of tubuloglomerular feedback responsiveness. We recently found that macula densa expresses α -, β and γ -splice variants neuronal nitric synthase 1 (NOS1), NOS1 expression in the increases on a high-salt diet. This study tested whether upregulation affects sodium excretion salt-sensitive hypertension by decreasing Expression levels mRNA protein were 30- five-fold higher, respectively, than those renal cortex C57BL/6 mice. Furthermore, NO...
Previous studies by our lab have established that placental-ischemia stimulated T-helper 17 cells (TH17s) cause increased cytolytic natural killer (cNK) cell proliferation and activation during pregnancy; however, the exact mechanism is unknown. The objective of this study was to investigate role interlukin (IL-17) in inducing cNK pregnancy. We infused 150 pg/day recombinant IL-17 into a subset normal pregnant (NP) Sprague Dawley rats from gestation day (GD) 12–19 (NP+IL-17). On GD 19, mean...
A chimeric immune receptor consisting of an extracellular antigen-binding domain derived from the CC49 humanized single-chain antibody, linked to CD3zeta signaling T cell receptor, was generated (CC49-zeta). This binds TAG-72, a mucin antigen expressed by most human adenocarcinomas. CC49-zeta in CD4+ and CD8+ cells induced cytokine production on stimulation. Human expressing recognized killed tumor lines primary TAG-72. did not mediate bystander killing TAG-72-negative cells. In addition,...
We have reported that the myogenic response of renal afferent arteriole (Af-art) and middle cerebral artery (MCA) autoregulation blood flow are impaired in Fawn-Hooded Hypertensive (FHH) rats. Transfer a region chromosome 1 containing γ-adducin (Add3) from Brown Norway rat rescued vascular dysfunction development disease. To examine whether Add3 is viable candidate gene altering hemodynamics FHH rats, we knocked down expression Af-arts MCAs cultured for 36-h using 27-mer Dicer-substrate...
The key myeloid transcription factor (TF), CEBPA, is frequently mutated in acute leukemia (AML), but the direct molecular effects of this leukemic driver mutation remain elusive. To investigate CEBPA mutant AML, we performed microscale, vivo chromatin immunoprecipitation sequencing and identified a set aberrantly activated enhancers, exclusively occupied by leukemia-associated CEBPA-p30 isoform. Comparing gene expression changes human AML corresponding CebpaLp30 mouse model, Nt5e, encoding...
Background: Neurocysticercosis (NCC) is the most common helminthic infection of central nervous system (CNS). The diagnosis NCC sometimes challenging due to its heterogenous clinical manifestations and variable sensitivity specificity neuroimaging serological tests. Methods: Next-generation sequencing (NGS) cerebrospinal fluid (CSF) was used detect pathogens in patients with clinically suspected CNS infections. A series diagnosed reviewed here. Results: Using NGS CSF, four were NCC. reads...
Activation of interferon genes constitutes an important anticancer pathway able to restrict proliferation cancer cells. Here, we demonstrate that the H3K9me3 histone methyltransferase (HMT) suppressor variegation 3-9 homolog 1 (SUV39H1) is required for acute myeloid leukemia (AML) and find its loss leads activation pathway. Mechanistically, show this occurs via destabilization a complex composed SUV39H1 two H3K9me2 HMTs, G9A GLP. Indeed, correlated with key genes, interference activities...
Epigenetic regulators are frequently mutated in hematological malignancies including acute myeloid leukemia (AML). Thus, the identification and characterization of novel epigenetic drivers affecting AML biology holds potential to improve our basic understanding uncover options for therapeutic intervention. To identify tumor suppressive AML, we performed an vivo short hairpin RNA (shRNA) screen context CEBPA mutant AML. This identified Histone 3 Lysine 4 (H3K4) demethylase KDM5C as a...
Eggshell colour is the unique appearance and economically valuable trait of eggs, whereas often short uniformity, especially in blue-shelled breeds, hence, their pigment differences molecular mechanism need clarity. To investigate relationship between content eggshells related gene expression eggshell glands chickens, four subtypes eggs ('Olive', 'Green', 'Blue', 'Light') from same blue-eggshell chicken line were selected; Hy-Line 'White' 'Brown'-shelled used as control groups. The L*, a*,...